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We have developed a multimodal photoacoustic remote sensing (PARS) microscope combined with swept source optical coherence tomography for in vivo, non-contact retinal imaging. Building on the proven strength of multiwavelength PARS imaging,…

We present the first label-free, non-contact, in-vivo imaging of the ocular vasculature using photoacoustic remote sensing (PARS) microscopy. Both anterior and posterior segments mouse eye were imaged. Vasculature of iris, sclera and retina…

Vascular imaging is critical for understanding human health and disease. Most established non-contact and label-free optical techniques capture predominantly structural information about vasculature. However, in many pathologies, functional…

Histological images are critical in the diagnosis and treatment of cancers. Unfortunately, the current method for capturing these microscopy images require resource intensive tissue preparation that delays diagnosis for many days to a few…

Label-free optical absorption microscopy techniques have evolved as effective tools for non-invasive chemical specific structural, and functional imaging. Yet most modern label-free microscopy modalities target only a fraction of the…

Label-free optical absorption microscopy techniques continue to evolve as promising tools for label-free histopathological imaging of cells and tissues. However, critical challenges relating to specificity and contrast, as compared to…

Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging.…

Mohs micrographic surgery (MMS) is a precise oncological technique where layers of tissue are resected and examined with intraoperative histopathology to minimize the removal of normal tissue while completely excising the cancer. To achieve…

Optical Coherence Tomography (OCT) is one of the most emerging imaging modalities that has been used widely in the field of biomedical imaging. From its emergence in 1990's, plenty of hardware and software improvements have been made. Its…

计算机视觉与模式识别 · 计算机科学 2014-11-18 Ahmadreza Baghaie , Roshan M. D'souza , Zeyun Yu

Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques in mice have limitations due to handling restrictions, suboptimal optical properties,…

Intrinsic optical signal (IOS) imaging of the retina, also termed as optoretinography (ORG), promises a noninvasive method for objective assessment of retinal function. By providing unparalleled capability to differentiate individual layers…

医学物理 · 物理学 2022-02-22 Tae-Hoon Kim , Guangying Ma , Taeyoon Son , Xincheng Yao

Advances in optical coherence tomography (OCT) have enabled noninvasive imaging of substructures of the human retina with high spatial resolution. OCT examinations are now a standard procedure in clinics and an integral part of ophthalmic…

人机交互 · 计算机科学 2022-12-22 Martin Röhlig , Oliver Stachs , Heidrun Schumann

Purpose: The integration of multimodal imaging into operating rooms paves the way for comprehensive surgical scene understanding. In ophthalmic surgery, by now, two complementary imaging modalities are available: operating microscope (OPMI)…

计算机视觉与模式识别 · 计算机科学 2026-03-27 Nikolo Rohrmoser , Ghazal Ghazaei , Michael Sommersperger , Nassir Navab

One of the greatest needs in modern ophthalmology is access to the eye choroid in vivo. Without it, it is difficult to introduce new therapies and to understand most of the pathological changes in the eye, because of the total blood flow in…

Optical tomographic cross-sectional images of biological samples were made possible by interferometric imaging techniques such as Optical Coherence Tomography (OCT). Owing to its unprecedented view of the sample, OCT has become a gold…

医学物理 · 物理学 2020-07-13 Pedro Mecê , Elena Gofas-Salas , Michel Paques , Kate Grieve , Serge Meimon

Optical coherence tomography (OCT) is a high-resolution three-dimensional imaging technique that enables non-destructive measurements of surface and subsurface microstructures. Recent developments of OCT operating in the mid-infrared (MIR)…

光学 · 物理学 2020-07-15 Ivan Zorin , Rong Su , Bettina Heise , Bernhard Lendl , Markus Brandstetter

High-resolution optical imaging methods, such as confocal microscopy and full-field optical coherence tomography, capture flat optical sections of the sample. If the sample is curved, the optical field sections through several sample layers…

Optical coherence tomography (OCT) uses low-coherence reflectometry to obtain cross-sectional images of inhomogeneous media, such as biological tissue. OCT is particularly useful in the biomedical ea, since the imaging can be performed…

医学物理 · 物理学 2018-03-01 Takahisa Mitsui , Kenichiro Aoki

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multi-photon microscopy for…

光学 · 物理学 2009-11-11 C. Vinegoni , T. Ralston , W. Tan , W. Luo , D. L. Marks , S. A. Boppart

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue, but lacks functional information. We present dynamic full-field optical coherence tomography to image living human induced…

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