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Quantitative phase microscopy (QPM) has found significant applications in the field of biomedical imaging which works on the principle of interferometry. The theory behind achieving interference in QPM with conventional light sources such…

光学 · 物理学 2021-07-26 Azeem Ahmad , Nikhil Jayakumar , Balpreet Singh Ahluwalia

White light phase-shifting interference microscopy (WL-PSIM) is a prominent technique for high-resolution quantitative phase imaging (QPI) of industrial and biological specimens. However, multiple interferograms with accurate phase-shifts…

图像与视频处理 · 电气工程与系统科学 2021-05-04 Sunil Bhatt , Ankit Butola , Sheetal Raosaheb Kanade , Anand Kumar , Dalip Singh Mehta

With applications ranging from metabolomics to histopathology, quantitative phase microscopy (QPM) is a powerful label-free imaging modality. Despite significant advances in fast multiplexed imaging sensors and deep-learning-based inverse…

In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase…

光学 · 物理学 2020-12-17 Xi Chen , Mikhail E. Kandel , Gabriel Popescu

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

光学 · 物理学 2025-01-20 Jose Angel Picazo-Bueno , Maciej Trusiak , Javier Garcia , Vicente Mico

Multi-spectral quantitative phase imaging (MS-QPI) is a cutting-edge label-free technique to determine the morphological changes, refractive index variations and spectroscopic information of the specimens. The bottleneck to implement this…

Quantitative phase imaging (QPI) is important in many applications such as microscopy and crystallography. To quantitatively reveal phase information, people could either employ interference to map phase distribution into intensity fringes,…

光学 · 物理学 2020-11-11 Xianye Li , Yafei sun , Yikang He , Xun Li , Baoqing Sun

Quantitative susceptibility mapping (QSM) has gained broad interests in the field by extracting biological tissue properties, predominantly myelin, iron and calcium from magnetic resonance imaging (MRI) phase measurements in vivo. Thereby,…

图像与视频处理 · 电气工程与系统科学 2019-12-12 Woojin Jung , Steffen Bollmann , Jongho Lee

Quantitative phase imaging (QPI) is an emerging label-free technique that produces images containing morphological and dynamical information without contrast agents. Unfortunately, the phase is wrapped in most imaging system. Phase…

图像与视频处理 · 电气工程与系统科学 2021-09-01 Fangshu Yang , Thanh-an Pham , Nathalie Brandenberg , Matthias P. Lutolf , Jianwei Ma , Michael Unser

Tissue biopsy evaluation in the clinic is in need of quantitative disease markers for diagnosis and, most importantly, prognosis. Among the new technologies, quantitative phase imaging (QPI) has demonstrated promise for histopathology…

This article presents a thorough comparison of themain QPM techniques, focusing on their accuracy in terms of measurement precision and trueness. We focus on 8 techniques, namely digital holographic microscopy (DHM), cross-grating wavefront…

We present a data-driven approach to compensate for optical aberration in calibration-free quantitative phase imaging (QPI). Unlike existing methods that require additional measurements or a background region to correct aberrations, we…

图像与视频处理 · 电气工程与系统科学 2020-12-02 Taean Chang , Youngju Jo , Gunho Choi , Donghun Ryu , Hyun-Seok Min , Yongkeun Park

Quantitative phase imaging (QPI) is a label-free technique that provides optical path length information for transparent specimens, finding utility in biology, materials science, and engineering. Here, we present quantitative phase imaging…

Quantitative susceptibility mapping (QSM) utilizes MRI signal phase to estimate local tissue susceptibility, which has been shown useful to provide novel image contrast and as biomarkers of abnormal tissue. QSM requires addressing a…

医学物理 · 物理学 2019-06-03 Juan Liu , Kevin M. Koch

In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has…

Purpose: Quantitative phase imaging (QPI) is a label-free technique that provides high-contrast images of tissues and cells without the use of chemicals or dyes. Accurate semantic segmentation of cells in QPI is essential for various…

图像与视频处理 · 电气工程与系统科学 2024-05-13 Zhuchen Shao , Mark A. Anastasio , Hua Li

Data limitation is a significant challenge in applying deep learning to medical images. Recently, the diffusion probabilistic model (DPM) has shown the potential to generate high-quality images by converting Gaussian random noise into…

计算机视觉与模式识别 · 计算机科学 2024-08-26 Sepehr Salem Ghahfarokhi , Tyrell To , Julie Jorns , Tina Yen , Bing Yu , Dong Hye Ye

Quantitative phase imaging (QPI) is a label-free computational imaging technique used in various fields, including biology and medical research. Modern QPI systems typically rely on digital processing using iterative algorithms for phase…

光学 · 物理学 2023-06-28 Yuhang Li , Yi Luo , Deniz Mengu , Bijie Bai , Aydogan Ozcan

Quantitative susceptibility mapping (QSM) is a useful magnetic resonance imaging (MRI) technique which provides spatial distribution of magnetic susceptibility values of tissues. QSMs can be obtained by deconvolving the dipole kernel from…

计算机视觉与模式识别 · 计算机科学 2020-12-08 Gyutaek Oh , Hyokyoung Bae , Hyun-Seo Ahn , Sung-Hong Park , Jong Chul Ye

We propose a simple all-in-line single-shot scheme for diagnostics of ultrashort laser pulses, consisting of a multi-mode fiber, a nonlinear crystal and a CCD camera. The system records a 2D spatial intensity pattern, from which the pulse…

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