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The ability to extrapolate gene expression dynamics in living single cells requires robust cell segmentation, and one of the challenges is the amorphous or irregularly shaped cell boundaries. To address this issue, we modified the U-Net…

定量方法 · 定量生物学 2020-01-17 Nanyan Zhu , Chen Liu , Zakary S. Singer , Tal Danino , Andrew F. Laine , Jia Guo

Living cell segmentation from bright-field light microscopy images is challenging due to the image complexity and temporal changes in the living cells. Recently developed deep learning (DL)-based methods became popular in medical and…

定量方法 · 定量生物学 2022-07-07 Ali Ghaznavi , Renata Rychtarikova , Mohammadmehdi Saberioon , Dalibor Stys

Segmentation, or the outlining of objects within images, is a critical step in the measurement and analysis of cells within microscopy images. While improvements continue to be made in tools that rely on classical methods for segmentation,…

定量方法 · 定量生物学 2024-03-15 Nodar Gogoberidze , Beth A. Cimini

Developing clinically useful cell-level analysis tools in digital pathology remains challenging due to limitations in dataset granularity, inconsistent annotations, high computational demands, and difficulties integrating new technologies…

计算机视觉与模式识别 · 计算机科学 2025-12-02 Nikita Shvetsov , Thomas K. Kilvaer , Masoud Tafavvoghi , Anders Sildnes , Kajsa Møllersen , Lill-Tove Rasmussen Busund , Lars Ailo Bongo

Imaging assays of cellular function, especially those using fluorescent stains, are ubiquitous in the biological and medical sciences. Despite advances in computer vision, such images are often analyzed using only manual or rudimentary…

计算机视觉与模式识别 · 计算机科学 2017-06-06 Lena R. Bartell , Lawrence J. Bonassar , Itai Cohen

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

计算机视觉与模式识别 · 计算机科学 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

Segmenting visual stimuli into distinct groups of features and visual objects is central to visual function. Classical psychophysical methods have helped uncover many rules of human perceptual segmentation, and recent progress in machine…

计算机视觉与模式识别 · 计算机科学 2023-10-12 Jonathan Vacher , Claire Launay , Pascal Mamassian , Ruben Coen-Cagli

Visual segmentation is a key perceptual function that partitions visual space and allows for detection, recognition and discrimination of objects in complex environments. The processes underlying human segmentation of natural images are…

计算机视觉与模式识别 · 计算机科学 2019-05-03 Jonathan Vacher , Pascal Mamassian , Ruben Coen-Cagli

MR imaging is a valuable diagnostic tool allowing to non-invasively visualize patient anatomy and pathology with high soft-tissue contrast. However, MRI acquisition is typically time-consuming, leading to patient discomfort and increased…

图像与视频处理 · 电气工程与系统科学 2025-12-23 Jan Nikolas Morshuis , Matthias Hein , Christian F. Baumgartner

Specular microscopy assessment of the human corneal endothelium (CE) in Fuchs' dystrophy is challenging due to the presence of dark image regions called guttae. This paper proposes a UNet-based segmentation approach that requires minimal…

Size uniformity is one of the main criteria of superpixel methods. But size uniformity rarely conforms to the varying content of an image. The chosen size of the superpixels therefore represents a compromise - how to obtain the fewest…

计算机视觉与模式识别 · 计算机科学 2016-11-29 Radhakrishna Achanta , Pablo Márquez-Neila , Pascal Fua , Sabine Süsstrunk

To segment 4K or 6K ultra high-resolution images needs extra computation consideration in image segmentation. Common strategies, such as down-sampling, patch cropping, and cascade model, cannot address well the balance issue between…

计算机视觉与模式识别 · 计算机科学 2025-02-24 Tiancheng Shen , Yuechen Zhang , Lu Qi , Jason Kuen , Xingyu Xie , Jianlong Wu , Zhe Lin , Jiaya Jia

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

计算机视觉与模式识别 · 计算机科学 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

Unsupervised evaluation of segmentation quality is a crucial step in image segmentation applications. Previous unsupervised evaluation methods usually lacked the adaptability to multi-scale segmentation. A scale-constrained evaluation…

计算机视觉与模式识别 · 计算机科学 2016-11-16 Yuhang Lu , Youchuan Wan , Gang Li

Vascular segmentation extracts blood vessels from images and serves as the basis for diagnosing various diseases, like ophthalmic diseases. Ophthalmologists often require high-resolution segmentation results for analysis, which leads to…

图像与视频处理 · 电气工程与系统科学 2022-07-29 Yan Hu , Zhongxi Qiu , Dan Zeng , Li Jiang , Chen Lin , Jiang Liu

In the domain of battery research, the processing of high-resolution microscopy images is a challenging task, as it involves dealing with complex images and requires a prior understanding of the components involved. The utilization of deep…

计算机视觉与模式识别 · 计算机科学 2025-03-19 Ganesh Raghavendran , Bing Han , Fortune Adekogbe , Shuang Bai , Bingyu Lu , William Wu , Minghao Zhang , Ying Shirley Meng

Despite the superior performance of Deep Learning (DL) on numerous segmentation tasks, the DL-based approaches are notoriously overconfident about their prediction with highly polarized label probability. This is often not desirable for…

图像与视频处理 · 电气工程与系统科学 2021-12-14 Sungmin Hong , Anna K. Bonkhoff , Andrew Hoopes , Martin Bretzner , Markus D. Schirmer , Anne-Katrin Giese , Adrian V. Dalca , Polina Golland , Natalia S. Rost

Super-resolution results are usually measured by full-reference image quality metrics or human rating scores. However, these evaluation methods are general image quality measurement, and do not account for the nature of the super-resolution…

计算机视觉与模式识别 · 计算机科学 2023-03-14 Sheng Cheng

The segmentation of digital images is one of the essential steps in image processing or a computer vision system. It helps in separating the pixels into different regions according to their intensity level. A large number of segmentation…

计算机视觉与模式识别 · 计算机科学 2019-09-12 Amit Gurung , Sangyal Lama Tamang

Contemporary approaches to instance segmentation in cell science use 2D or 3D convolutional networks depending on the experiment and data structures. However, limitations in microscopy systems or efforts to prevent phototoxicity commonly…

计算机视觉与模式识别 · 计算机科学 2023-01-27 Christopher Z. Eddy , Austin Naylor , Bo Sun