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This paper introduces a new method of data-driven microscope design for virtual fluorescence microscopy. Our results show that by including a model of illumination within the first layers of a deep convolutional neural network, it is…

图像与视频处理 · 电气工程与系统科学 2020-04-23 Colin L. Cooke , Fanjie Kong , Amey Chaware , Kevin C. Zhou , Kanghyun Kim , Rong Xu , D. Michael Ando , Samuel J. Yang , Pavan Chandra Konda , Roarke Horstmeyer

The size- and fluorescence-based sorting of micro- and nano-scale particles suspended in fluid presents a significant and important challenge for both sample analysis and for manufacturing of nanoparticle-based products. Here we demonstrate…

软凝聚态物质 · 物理学 2015-02-16 Sukumar Rajauria , Christopher Axline , Claudia Gottstein , Andrew N. Cleland

Preliminary diagnosis of fungal infections can rely on microscopic examination. However, in many cases, it does not allow unambiguous identification of the species by microbiologist due to their visual similarity. Therefore, it is usually…

计算机视觉与模式识别 · 计算机科学 2020-09-09 Bartosz Zieliński , Agnieszka Sroka-Oleksiak , Dawid Rymarczyk , Adam Piekarczyk , Monika Brzychczy-Włoch

Biclustering is an unsupervised data mining technique that aims to unveil patterns (biclusters) from gene expression data matrices. In the framework of this thesis, we propose new biclustering algorithms for microarray data. The latter is…

机器学习 · 计算机科学 2018-11-26 Amina Houari

Microtubules are filament-shaped, polymeric proteins (~25 nm in diameter) involved in cellular structure and organization. We demonstrate the imaging of individual microtubules using a conventional bright-field microscope, without any…

生物物理 · 物理学 2015-05-14 Braulio Gutiérrez-Medina , Steven M. Block

Microscopy image enhancement plays a pivotal role in understanding the details of biological cells and materials at microscopic scales. In recent years, there has been a significant rise in the advancement of microscopy image enhancement,…

图像与视频处理 · 电气工程与系统科学 2025-09-29 Debasish Dutta , Neeharika Sonowal , Risheraj Barauh , Deepjyoti Chetia , Sanjib Kr Kalita

In this paper, a semi-automatic annotation of bacteria genera and species from DIBaS dataset is implemented using clustering and thresholding algorithms. A Deep learning model is trained to achieve the semantic segmentation and…

图像与视频处理 · 电气工程与系统科学 2022-08-24 Chethan Reddy G. P. , Pullagurla Abhijith Reddy , Vidyashree R. Kanabur , Deepu Vijayasenan , Sumam S. David , Sreejith Govindan

Robust and accurate nuclei centroid detection is important for the understanding of biological structures in fluorescence microscopy images. Existing automated nuclei localization methods face three main challenges: (1) Most of object…

图像与视频处理 · 电气工程与系统科学 2021-11-05 Liming Wu , Shuo Han , Alain Chen , Paul Salama , Kenneth W. Dunn , Edward J. Delp

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

Machine learning-based approaches outperform competing methods in most disciplines relevant to diagnostic radiology. Interventional radiology, however, has not yet benefited substantially from the advent of deep learning, in particular…

Cell segmentation is a fundamental task for computational biology analysis. Identifying the cell instances is often the first step in various downstream biomedical studies. However, many cell segmentation algorithms, including the recently…

计算机视觉与模式识别 · 计算机科学 2022-12-08 Gihun Lee , Sangmook Kim , Joonkee Kim , Se-Young Yun

Fluorescence microscopy is essential in biological and medical research, providing critical insights into cellular structures. However, limited by optical diffraction and background noise, a substantial amount of hidden information is still…

生物物理 · 物理学 2026-04-14 Xiaofeng Zhang , Yongsheng Huang , Jielong Yang , Zhili Wang , Si Chen , Linbo Liu , Xin Ge

In x-ray microscopy, traditional raster-scanning techniques are used to acquire a microscopic image in a series of step-scans. Alternatively, scanning the x-ray probe along a continuous path, called a fly-scan, reduces scan time and…

图像与视频处理 · 电气工程与系统科学 2025-09-23 Yu Lu , Thomas F. Lynn , Ming Du , Zichao Di , Sven Leyffer

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

Microarray is one of the essential technologies used by the biologist to measure genome-wide expression levels of genes in a particular organism under some particular conditions or stimuli. As microarrays technologies have become more…

人工智能 · 计算机科学 2020-05-05 Khalid Raza

Automatic detection and tracking of cells in microscopy images are major applications of computer vision technologies in both biomedical research and clinical practice. Though machine learning methods are increasingly common in these…

计算机视觉与模式识别 · 计算机科学 2023-12-07 Nikomidisz Eftimiu , Michal Kozubek

Methods: We have developed a software suite (DataSet Tracker) for real-time analysis designed to run on computers, smartphones, and smart glasses hardware and suitable for resource-constrained, on-the-fly computing in microscopes without…

定量方法 · 定量生物学 2025-08-13 Alexandre Matov

How microorganisms respond to and interact with their environment can vary significantly from individual to individual, which can have important microbiological and ecological implications. However, most microscopy techniques can only…

This paper presents a systematic solution for the intelligent recognition and automatic analysis of microscopy images. We developed a data engine that generates high-quality annotated datasets through a combination of the collection of…

图像与视频处理 · 电气工程与系统科学 2025-08-27 Yanhui Hong , Nan Wang , Zhiyi Xia , Haoyi Tao , Xi Fang , Yiming Li , Jiankun Wang , Peng Jin , Xiaochen Cai , Shengyu Li , Ziqi Chen , Zezhong Zhang , Guolin Ke , Linfeng Zhang

The need for single-camera 3D particle tracking methods is growing, among others, due to the increasing focus in biomedical research often relying on single-plane microscopy imaging. Defocusing-based methods are ideal for a wide-spread use…

流体动力学 · 物理学 2021-08-04 Rune Barnkob , Massimiliano Rossi