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Vascular imaging is critical for understanding human health and disease. Most established non-contact and label-free optical techniques capture predominantly structural information about vasculature. However, in many pathologies, functional…

Early diagnosis of ocular diseases improves the understanding of pathophysiology and helps with accurate monitoring and effective treatment. Advanced multimodal ocular imaging platforms play a crucial role in the visualization of the ocular…

We have developed a multimodal photoacoustic remote sensing (PARS) microscope combined with swept source optical coherence tomography for in vivo, non-contact retinal imaging. Building on the proven strength of multiwavelength PARS imaging,…

Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging.…

Label-free optical absorption microscopy techniques have evolved as effective tools for non-invasive chemical specific structural, and functional imaging. Yet most modern label-free microscopy modalities target only a fraction of the…

Histological images are critical in the diagnosis and treatment of cancers. Unfortunately, the current method for capturing these microscopy images require resource intensive tissue preparation that delays diagnosis for many days to a few…

Label-free optical absorption microscopy techniques continue to evolve as promising tools for label-free histopathological imaging of cells and tissues. However, critical challenges relating to specificity and contrast, as compared to…

In the past decades, absorption modalities have emerged as powerful tools for label-free functional and structural imaging of cells and tissues. Many biomolecules present unique absorption spectra providing chromophore-specific information…

Today's diagnostics include devices such as pulse oximeters, blood pressure monitors, and temperature measurements. These devices provide vital information to medical personnel when making treatment decisions. Drawing inspiration from the…

图像与视频处理 · 电气工程与系统科学 2022-08-26 Akhil Kallepalli , David B James , Mark A Richardson

Photoacoustic Remote Sensing (PARS) microscopy is an emerging label-free optical absorption imaging modality. PARS operates by capturing nanosecond-scale optical perturbations generated by photoacoustic pressures. These time-domain (TD)…

医学物理 · 物理学 2022-08-17 Nicholas Pellegrino , Benjamin R. Ecclestone , Paul Fieguth , Parsin Haji Reza

Mohs micrographic surgery (MMS) is a precise oncological technique where layers of tissue are resected and examined with intraoperative histopathology to minimize the removal of normal tissue while completely excising the cancer. To achieve…

Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

Significance: Histopathological analysis of tissues is an essential tool for grading, staging, diagnosing and resecting cancers and other malignancies. Current histopathological techniques require substantial sample processing prior to…

医学物理 · 物理学 2021-07-14 Benjamin Ecclestone , Deepak Dinakaran , Parsin Haji Reza

Photoacoustic imaging has emerged as a powerful, non-invasive modality for various biomedical applications. Conventional photoacoustic systems require contact-based ultrasound detection and expensive and bulky high-power lasers for the…

应用物理 · 物理学 2025-01-06 Emiel Dieussaert , Roel Baets , Hilde Jans , Xavier Rottenberg , Yanlu Li

Photon Absorption Remote Sensing (PARS) enables label-free imaging of subcellular morphology by observing biomolecule specific absorption interactions. Coupled with deep-learning, PARS produces label-free virtual Hematoxylin and Eosin (H&E)…

Various morphological and functional parameters of peripheral nerves and their vascular supply are indicative of pathological changes due to injury or disease. Based on recent improvements in optoacoustic image quality, we explore the…

图像与视频处理 · 电气工程与系统科学 2022-07-29 Dominik Jüstel , Hedwig Irl , Florian Hinterwimmer , Christoph Dehner , Walter Simson , Nassir Navab , Gerhard Schneider , Vasilis Ntziachristos

Histopathological visualizations are a pillar of modern medicine and biological research. Surgical oncology relies exclusively on post-operative histology to determine definitive surgical success and guide adjuvant treatments. The current…

图像与视频处理 · 电气工程与系统科学 2022-04-04 Marian Boktor , Benjamin Ecclestone , Vlad Pekar , Deepak Dinakaran , John R. Mackey , Paul Fieguth , Parsin Haji Reza

This paper presents a non-contact vital signs (respiration and heartbeat) monitoring system that utilizes visible light sensing (VLS) technology. We have for the first time demonstrated the ability to wirelessly (non-contact) sense vital…

信号处理 · 电气工程与系统科学 2019-12-17 Hisham Abuella , Sabit Ekin

We present a photoacoustic computed tomography investigation on a healthy human finger, to image blood vessels with a focus on vascularity across the interphalangeal joints. The cross-sectional images were acquired using an imager…

The mechanical properties of micro-scale bio-entities are fundamental for understanding their functions and pathological states. However, current methods for assessing elastic properties at single-particle level such as Brillouin and atomic…

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