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Inspired by recent experiments on the effects of cytosolic crowders on the organization of bacterial chromosomes, we consider a "feather-boa" type model chromosome in the presence of non-additive crowders, encapsulated within a cylindrical…

生物物理 · 物理学 2020-02-11 Amit Kumar , Pinaki Swain , Bela M. Mulder , Debasish Chaudhuri

Biomolecular condensates are essential for cellular organization and result from phase separation in systems far from thermodynamic equilibrium. Among various models, chemically active droplets play a significant role, consisting of…

软凝聚态物质 · 物理学 2025-05-19 Jacques Fries , Roxanne Berthin , Chengjie Luo , Marie Jardat , David Zwicker , Vincent Dahirel , Pierre Illien

Macromolecular crowding plays a principal role in a wide range of biological processes including gene expression, chromosomal compaction, and viral infection. However, the impact that crowding has on the dynamics of nucleic acids remains a…

Spindles are self-organized microtubule-based structures that segregate chromosomes during cell division. The mass of the spindle is controlled by the balance between microtubule turnover and nucleation. The mechanisms that control the…

The dynamic behavior of microtubules in solution can be strongly modified by interactions with walls or other structures. We examine here a microtubule growth model where the increase in size of the plus-end is perturbed by collisions with…

软凝聚态物质 · 物理学 2010-09-29 Vladimir A. Baulin , Carlos M. Marques , Fabrice Thalmann

Microtubules, major elements of the cell skeleton are, most of the time, well organized in vivo, but they can also show self-organizing behaviors in time and/or space in purified solutions in vitro. Theoretical studies and models based on…

生物物理 · 物理学 2012-09-03 Nicolas Glade

The looping of polymers such as DNA is a fundamental process in the molecular biology of living cells, whose interior is characterised by a high degree of molecular crowding. We here investigate in detail the looping dynamics of flexible…

软凝聚态物质 · 物理学 2014-12-24 Jaeoh Shin , Andrey G. Cherstvy , Ralf Metzler

Motivated by recent experiments probing shape, size and dynamics of bacterial chromosomes in growing cells, we consider a polymer model consisting of a circular backbone to which side-loops are attached, confined to a cylindrical cell. Such…

生物物理 · 物理学 2019-05-13 Pinaki Swain , Bela M. Mulder , Debasish Chaudhuri

Macromolecular diffusion in dense colloidal suspensions is an intriguing topic of interdisciplinary relevance in Science and Engineering. While significant efforts have been undertaken to establish the impact of crowding on the dynamics of…

软凝聚态物质 · 物理学 2021-06-01 Luca Tonti , Fabián A. García Daza , Alessandro Patti

We showed in our previous studies that just $3\%$ cross-links, at special points along the contour of the bacterial DNA help the DNA-polymer to get organized at micron length scales \cite{jpcm,epl}. In this work, we investigate how does the…

软凝聚态物质 · 物理学 2019-05-01 Tejal Agarwal , G. P. Manjunath , Farhat Habib , Apratim Chatterji

The activity of biological cells is primarily based on chemical reactions and typically modeled as a reaction-diffusion system. Cells are, however, highly crowded with macromolecules, including a variety of molecular machines such as…

生物物理 · 物理学 2018-11-02 Yuichi Togashi

Gradient-driven diffusion in crowded, multicomponent mixtures is a topic of high interest because of its role in biological processes such as transport in cell membranes. In partially phase-separated solutions, gradient-driven diffusion…

软凝聚态物质 · 物理学 2017-02-15 Prithviraj Nandigrami , Brandy Grove , Andrew Konya , Robin L. B. Selinger

How thousands of microtubules and molecular motors self-organize into spindles remains poorly understood. By combining static, nanometer-resolution, large-scale electron tomography reconstructions and dynamic, optical-resolution, polarized…

During the life cycle of bacterial cells the non-mixing of the two ring-shaped daughter genomes is an important prerequisite for the cell division process. Mimicking the environments inside highly crowded biological cells, we study the…

软凝聚态物质 · 物理学 2014-12-24 Jaeoh Shin , Andrey G Cherstvy , Ralf Metzler

Crowded environments modify the diffusion of macromolecules, generally slowing their movement and inducing transient anomalous subdiffusion. The presence of obstacles also modifies the kinetics and equilibrium behavior of tracers. While…

Intracellular transport of vesicular cargos, organelles, and other macromolecules is an essential process to move large items through a crowded, and inhomogeneous cellular environment. In an effort to dissect the fundamental effects of…

生物大分子 · 定量生物学 2014-09-12 Leslie Conway , Jennifer L. Ross

The cytoplasm of a living cell is crowded with several macromolecules of different shapes and sizes. Molecular diffusion in such a medium becomes anomalous due to the presence of macromolecules and diffusivity is expected to decrease with…

统计力学 · 物理学 2009-11-13 Manish Agrawal , S. B. Santra , Rajat Anand , Rajaram Swaminathan

Tubular lattices are ubiquitous in nature and technology. Microtubules and nanotubes of all kinds act as important pillars of biological cells and the man-made nano-world. We show that when prestress is introduced in such structures,…

软凝聚态物质 · 物理学 2013-12-12 Osman Kahraman , Hervé Mohrbach , Martin Michael Müller , Igor M. Kulić

The genomic DNA of bacteria occupies only a fraction of the cell called the nucleoid, although it is not bounded by any membrane and would occupy a volume hundreds of times larger than the cell in the absence of constraints. The two most…

生物物理 · 物理学 2023-11-13 Marc Joyeux

The mitotic spindle lies at the heart of the spatio-temporal control over cellular components during cell division. The spindle consists of microtubules, which are not only crosslinked by motor proteins but also by passive binding proteins.…

生物物理 · 物理学 2020-08-19 Harmen Wierenga , Pieter Rein ten Wolde
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