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Laser Doppler holography (LDH) is a full-field imaging technique that was recently used in the human eye to reveal blood flow contrasts in the retinal and choroidal vasculature non-invasively, and with high temporal resolution. We here…

医学物理 · 物理学 2020-04-01 L. Puyo , M. Paques , M. Fink , J-A Sahel , M. Atlan

Laser Doppler holography (LDH) is a full-field blood flow imaging technique able to reveal human retinal and choroidal blood flow with high temporal resolution. We here report on using LDH in the anterior segment of the eye without making…

Laser Doppler holography (LDH) is a full-field interferometric imaging technique recently applied in ophthalmology to measure blood flow, a parameter of high clinical interest. From the temporal fluctuations of digital holograms acquired at…

图像与视频处理 · 电气工程与系统科学 2020-05-13 Leo Puyo , Michel Paques , Michael Atlan

Laser Doppler holography is a planar blood flow imaging technique recently introduced in ophthalmology to image human retinal and choroidal blood flow non-invasively. Here we present a digital method based on the Doppler spectrum asymmetry…

医学物理 · 物理学 2021-08-10 Léo Puyo , Michel Paques , Michael Atlan

Full-field swept-source optical coherence tomography (FF-SS-OCT) and laser Doppler holography (LDH) are two holographic imaging techniques presenting unique capabilities for ophthalmology. We report on interlaced FF-SS-OCT and LDH imaging…

医学物理 · 物理学 2022-03-01 Léo Puyo , Hendrik Spahr , Clara Pfäffle , Gereon Hüttmann , Dierck Hillmann

Laser Doppler holography was introduced as a full-field imaging technique to measure blood flow in the retina and choroid with an as yet unrivaled temporal resolution. We here investigate separating the different contributions to the power…

医学物理 · 物理学 2021-06-02 Léo Puyo , Michel Paques , Mathias Fink , José-Alain Sahel , Michael Atlan

The eye offers a unique opportunity for non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome…

医学物理 · 物理学 2018-09-18 L. Puyo , M. Paques , M. Fink , J. -A. Sahel , M. Atlan

We report on local superficial blood flow monitoring in biological tissue from laser Doppler holographic imaging. In time averaging recording conditions, holography acts as a narrowband bandpass filter, which, combined with a frequency…

High-speed digital holography reveals local blood flow contrasts in the eye fundus through deterministic signal analysis, leveraging a forward scattering model of dynamically diffused light. This approach enables the estimation of absolute…

仪器与探测器 · 物理学 2025-05-14 Zacharie Auray , Yann Fischer , Olivier Martinache , Michael Atlan

This study presents a novel quantitative estimation method for total retinal arterial blood flow utilizing real-time Doppler holography at an unprecedented frame rate of 33,000 frames per second. This technique, leveraging high-speed…

图像与视频处理 · 电气工程与系统科学 2024-09-27 Yann Fischer , Zacharie Auray , Olivier Martinache , Marius Dubosc , Noé Topéza , Chloé Magnier , Maxime Boy-Arnould , Michael Atlan

A comprehensive assessment of retinal health demands reliable and precise methods to measure localized blood perfusion. Despite considerable advancements in imaging techniques, such as indocyanine green and fluorescein angiography, along…

医学物理 · 物理学 2024-03-15 Michael Atlan

We performed non-invasive video imaging of retinal blood flow in a pigmented rat by holographic interferometry of near-infrared laser light backscattered by retinal tissue, beating against an off-axis reference beam sampled at a frame rate…

The choroid is a highly vascularized tissues supplying the retinal pigment epithelium and photoreceptors. Its implication in retinal diseases is gaining increasing interest. However, investigating the anatomy and flow of the choroid remains…

医学物理 · 物理学 2021-06-02 Léo Puyo , Michel Paques , Mathias Fink , José-Alain Sahel , Michael Atlan

We report laser Doppler ophthalmoscopic fundus imaging in the rat eye with near-IR heterodyne holography. Sequential sampling of the beat of the reflected radiation against a frequency-shifted optical local oscillator is made onto an array…

We report a demonstration of laser Doppler holography at a sustained acquisition rate of 250 Hz on a 1 Megapixel complementary metal-oxide-semiconductor (CMOS) sensor array and image display at 10 Hz frame rate. The holograms are optically…

光学 · 物理学 2015-06-12 Benjamin Samson , Michael Atlan

We report on wide-field imaging of pulsatile motion induced by blood flow using heterodyne holographic interferometry on the thumb of a healthy volunteer, in real-time. Optical Doppler images were measured with green laser light by a…

光学 · 物理学 2015-04-01 Jeffrey Bencteux , Pierre Pagnoux , Thomas Kostas , Sam Bayat , Michael Atlan

We report on wide-field imaging of pulsatile microvascular blood flow in the exposed cerebral cortex of a mouse by holographic interferometry. We recorded interferograms of laser light backscattered by the tissue, beating against an…

医学物理 · 物理学 2015-10-08 L. Puyo , I. Ferezou , A. Rancillac , M. Simonutti , M. Paques , J. A. Sahel , M. Fink , M. Atlan

Heterodyne Holography is a variant of Digital Holography, where the optical frequencies of signal and reference arms can be freely adjusted by acousto-optic modulators. Heterodyne Holography is an extremely versatile and reliable…

光学 · 物理学 2012-06-08 Michel Gross , Frédéric Verpillat , Fadwa Joud , Michael Atlan

We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and…

光学 · 物理学 2014-05-01 Nicolas Verrier , Daniel Alexandre , Michel Gross

Due to hardware constraints, standard off-the-shelf digital cameras suffers from low dynamic range (LDR) and low frame per second (FPS) outputs. Previous works in high dynamic range (HDR) video reconstruction uses sequence of alternating…

图像与视频处理 · 电气工程与系统科学 2022-10-11 Zeeshan Khan , Parth Shettiwar , Mukul Khanna , Shanmuganathan Raman
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