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The past decade has brought many innovations in optical design for 3D super-resolution imaging of point-like emitters, but these methods often focus on single-emitter localization precision as a performance metric. Here, we propose a simple…

光学 · 物理学 2022-09-28 James M. Jusuf , Matthew D. Lew

The coupling of nanostructures with emitters opens ways for the realization of man-made subwavelength light emitting elements. In this article, we investigate the modification of fluorescence when an emitter is placed close to a…

其他凝聚态物理 · 物理学 2009-11-13 S. Kuehn , G. Mori , M. Agio , V. Sandoghdar

Deterministically integrating single solid-state quantum emitters with photonic nanostructures serves as a key enabling resource in the context of photonic quantum technology. Due to the random spatial location of many widely-used…

光学 · 物理学 2021-05-14 Shunfa Liu , Kartik Srinivasan , Jin Liu

By using scattering in near field techniques, a microscope can be easily turned into a device measuring static and dynamic light scattering, very useful for the characterization of nanoparticle dispersions. Up to now, microscopy based…

光学 · 物理学 2015-05-13 D. Brogioli , D. Salerno , V. Cassina , F. Mantegazza

In single molecule orientation localization microscopy, valuable information about the orientation and longitudinal position of each molecule is often encoded in the shape of the point spread function (PSF). This shape, though, can be…

We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. A 22-fold…

Specialized applications of nanoparticles often call for particular, well-characterized particle size distributions in solution. But, this property can prove difficult to measure. High-throughput methods, such as dynamic light scattering,…

介观与纳米尺度物理 · 物理学 2021-09-24 Luke Melo , Angus Hui , Matt Kowal , Eric Boateng , Zahra Poursorkh , Edène Rocheron , Jake Wong , Ashton Christy , Edward Grant

We study the fundamental limit on the localization precision for a subwavelength scatterer embedded in a strongly scattering environment, using the external degrees of freedom provided by wavefront shaping. For a weakly scattering target,…

光学 · 物理学 2020-11-05 Dorian Bouchet , Rémi Carminati , Allard P. Mosk

In the past years, optical fluorescence microscopy (OFM) made steady progress towards increasing the localisation precision of fluorescent emitters in biological samples. The high precision achieved by these techniques has prompted new…

Standard deviation measurements of intensity profiles of stationary single fluorescent molecules are useful for studying axial localization, molecular orientation, and a fluorescence imaging system's spatial resolution. Here we report on…

定量方法 · 定量生物学 2015-05-13 Michael C. DeSantis , Shawn H. DeCenzo , Je-Luen Li , Y. M. Wang

Multifocal microscopy affords fast acquisition of microscopic 3D images. This is made possible using a multifocal grating optic, however this induces chromatic dispersion effects into the point spread function impacting image quality and…

光学 · 物理学 2020-08-25 M. Junaid Amin , Sabine Petry , Joshua W. Shaevitz , Haw Yang

Point-spread-function (PSF) engineering is a well-established computational imaging technique that uses phase masks and other optical elements to embed extra information (e.g., depth) into the images captured by conventional CMOS image…

计算机视觉与模式识别 · 计算机科学 2024-06-14 Sachin Shah , Matthew Albert Chan , Haoming Cai , Jingxi Chen , Sakshum Kulshrestha , Chahat Deep Singh , Yiannis Aloimonos , Christopher Metzler

Important applications of single-particle tracking (SPT) aim at deciphering the diffusion properties of single fluorescent nanoparticles immersed in heterogeneous environments, such as multi-cellular biological tissues. To maximize the…

We investigate a nonlinear localization microscopy method based on Rabi oscillations of single emitters. We demonstrate the fundamental working principle of this new technique using a cryogenic far-field experiment in which subwavelength…

量子物理 · 物理学 2010-12-21 Ilja Gerhardt , Gert Wrigge , Jaseuk Hwang , Gert Zumofen , Vahid Sandoghdar

Single-emitter microscopy has emerged as a promising method of imaging nanostructures with nanoscale resolution. This technique uses the centroid position of an emitters far-field radiation pattern to infer its position to a precision that…

光学 · 物理学 2016-11-03 Kangmook Lim , Chad Ropp , John Fourkas , Benjamin Shapiro , Edo Waks

Point-spread function of the probe forming optics ($PSF_{optics} $) is reported for the first time in an uncorrected (without multipole correctors) scanning electron microscope (SEM). In an SEM, the electron probe information is lost as the…

材料科学 · 物理学 2024-07-02 Surya Kamal , Richard K. Hailstone

Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

生物物理 · 物理学 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

The Lamb shift of a quantum emitter very close to a plasmonic nanostructure, mainly induced by the higher-order plasmonic dark modes, can be three or more orders of magnitude larger than that in the free space and it is ultra-sensitive to…

量子物理 · 物理学 2023-06-06 Zeyang Liao , Yuwei Lu , Xue-Hua Wang

Precise control of particle positioning is desirable in many optical propulsion and sorting applications. Here, we develop an integrated platform for particle manipulation consisting of a combined optical nanofibre and optical tweezers…

The wide-angle scattering of X-rays by anisotropic powders of nano-layered crystallites (nano-stacks) is addressed. Assuming that the orientation distribution probability function f of the nano-stacks only depends on the deviation of the…

材料科学 · 物理学 2007-05-23 Yves Meheust , Kenneth Dahl Knudsen , Jon Otto Fossum