相关论文: Effects of excitation light polarization on fluore…
Light-sheet fluorescence microscopy (LSFM) makes use of a thin plane of light to optically section and image transparent tissues or organisms {\it{in vivo}}, which has the advantages of fast imaging speed and low phototoxicity. In this…
In light-sheet fluorescence microscopy (LSFM), the axial resolution is governed by the illumination beam profile, motivating the development of advanced beam-shaping techniques to enhance imaging performance. Two-photon LSFM (2P-LSFM), in…
Multiphoton microscopy is widely used for live imaging. However, its acquisition speed remains limited by fluorophore emission rates and photodamage. To increase the pixel rate of a two-photon microscope beyond a few megahertz (MHz),…
We present high resolution two-photon excitation microscopy studies combining two-photon fluorescence (TPF) and second harmonic generation (SHG) in order to probe orientational distributions of molecular ensembles at room temperature. A…
Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The…
Electronic 2D spectroscopy allows nontrivial quantum effects in chemistry and biology to be explored in unprecedented detail. Here, we apply recently developed fluorescence detected coherent 2D spectroscopy to study the light harvesting…
The femtosecond laser filamentation is the result of the dynamic interplay between plasma self-focusing and defocusing generated by the multiphoton/tunnel ionization of air molecules. This equilibrium allows the filament to stably propagate…
The polarization of light conveys crucial information about the spatial ordering and optical properties of a specimen. However, precise polarization measurement in challenging conditions, including constrained spaces, low light levels, and…
Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…
Nonsequential two-photon ionization of inner-shell $np$ subshell of neutral atoms by circularly polarized light is investigated. Detection of subsequent fluorescence as a signature of the process is proposed and the dependence of…
We study an inverse problem for Light Sheet Fluorescence Microscopy (LSFM), where the density of fluorescent molecules needs to be reconstructed. Our first step is to present a mathematical model to describe the measurements obtained by an…
A fraction of the light observed from edge-on disk galaxies is polarized due to two physical effects: selective extinction by dust grains aligned with the magnetic field, and scattering of the anisotropic starlight field. Since the…
We introduce a generalized theoretical approach to study action signals induced by the absorption of two-photons from two phase modulated laser beams and subject it to experimental testing for two types of photoactive samples, solution of…
It is shown that generalized measurements, required for optimally discriminating between nonorthogonal joint polarization states of two indistinguishable photons, can be realized with the help of polarization-dependent two-photon absorption…
Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light…
Two-photon absorption (TPA) fluorescence of biomarkers has been decisive in advancing the fields of biosensing and deep-tissue in vivo imaging of live specimens. However, due to the extremely small TPA cross section and the quadratic…
Laser-induced breakdown spectroscopy (LIBS) is a well-established technique widely used in fundamental research and diverse practical fields. Polarization-resolved LIBS, a variant of this technique, aims to improve the sensitivity, which is…
A universal and optimal method for the polarimetry of light with arbitrary photon statistics is presented. The method is based on the continuous maximum-likelihood positive operator-valued measure (ML-POVM) for pure polarization states over…
We describe the apparatus of a fluorescence optical microscope with both single-photon and two-photon non-diffracting light sheets excitation for large volume imaging. With special design to accommodate two different wavelength ranges…
Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however,…