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The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

定量方法 · 定量生物学 2022-10-05 André O. Françani

Motivation: Innovative microfluidic systems carry the promise to greatly facilitate spatio-temporal analysis of single cells under well-defined environmental conditions, allowing novel insights into population heterogeneity and opening new…

机器学习 · 计算机科学 2021-05-18 Dominik Stallmann , Jan P. Göpfert , Julian Schmitz , Alexander Grünberger , Barbara Hammer

We present a deep learning-based object detection and object tracking algorithm to study droplet motion in dense microfluidic emulsions. The deep learning procedure is shown to correctly predict the droplets' shape and track their motion at…

软凝聚态物质 · 物理学 2021-10-04 Mihir Durve , Fabio Bonaccorso , Andrea Montessori , Marco Lauricella , Adriano Tiribocchi , Sauro Succi

State-of-the-art image segmentation algorithms generally consist of at least two successive and distinct computations: a boundary detection process that uses local image information to classify image locations as boundaries between objects,…

计算机视觉与模式识别 · 计算机科学 2016-11-03 Michał Januszewski , Jeremy Maitin-Shepard , Peter Li , Jörgen Kornfeld , Winfried Denk , Viren Jain

Deep neural networks are rapidly emerging as data analysis tools, often outperforming the conventional techniques used in complex microfluidic systems. One fundamental analysis frequently desired in microfluidic experiments is counting and…

计算机视觉与模式识别 · 计算机科学 2022-08-17 Mihir Durve , Adriano Tiribocchi , Fabio Bonaccorso , Andrea Montessori , Marco Lauricella , Michal Bogdan , Jan Guzowski , Sauro Succi

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

计算机视觉与模式识别 · 计算机科学 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

This paper presents a method for time-lapse 3D cell analysis. Specifically, we consider the problem of accurately localizing and quantitatively analyzing sub-cellular features, and for tracking individual cells from time-lapse 3D confocal…

计算机视觉与模式识别 · 计算机科学 2022-08-18 Jiaxiang Jiang , Amil Khan , S. Shailja , Samuel A. Belteton , Michael Goebel , Daniel B. Szymanski , B. S. Manjunath

Artificial intelligence is nowadays used for cell detection and classification in optical microscopy, during post-acquisition analysis. The microscopes are now fully automated and next expected to be smart, to make acquisition decisions…

Automated in-vitro cell detection and counting have been a key theme for artificial and intelligent biological analysis such as biopsy, drug analysis and decease diagnosis. Along with the rapid development of microfluidics and lab-on-chip…

计算机视觉与模式识别 · 计算机科学 2020-01-08 Tiancheng Xia , Richard Jiang , YongQing Fu , Nanlin Jin

Computer vision algorithms with pixel-wise labeling tasks, such as semantic segmentation and salient object detection, have gone through a significant accuracy increase with the incorporation of deep learning. Deep segmentation methods…

计算机视觉与模式识别 · 计算机科学 2018-05-23 Caglar Aytekin , Xingyang Ni , Francesco Cricri , Lixin Fan , Emre Aksu

Intense short-wavelength pulses from free-electron lasers and high-harmonic-generation sources enable diffractive imaging of individual nano-sized objects with a single x-ray laser shot. The enormous data sets with up to several million…

Cellular processes are governed by macromolecular complexes inside the cell. Study of the native structures of macromolecular complexes has been extremely difficult due to lack of data. With recent breakthroughs in Cellular electron cryo…

定量方法 · 定量生物学 2018-06-12 Chengqian Che , Ruogu Lin , Xiangrui Zeng , Karim Elmaaroufi , John Galeotti , Min Xu

Deep learning methods have typically been trained on large datasets in which many training examples are available. However, many real-world product datasets have only a small number of images available for each product. We explore the use…

计算机视觉与模式识别 · 计算机科学 2015-07-31 David Held , Sebastian Thrun , Silvio Savarese

Fluid ferroelectrics, a recently discovered class of liquid crystals that exhibit switchable, long-range polar order, offer opportunities in ultrafast electro-optic technologies, responsive soft matter, and next-generation energy materials.…

Accurate single cell detection in brightfield microscopy is crucial for biological research, yet data scarcity and annotation bottlenecks limit the progress of deep learning methods. We investigate the use of unconditional models to…

计算机视觉与模式识别 · 计算机科学 2025-12-02 Mario de Jesus da Graca , Jörg Dahlkemper , Peer Stelldinger

The advancement of the neuroscientific imaging techniques has produced an unprecedented size of neural cell imaging data, which calls for automated processing. In particular, identification of cells from two photon images demands…

图像与视频处理 · 电气工程与系统科学 2019-09-26 Si-Baek Seong , Hae-Jeong Park

Advanced microscopy and/or spectroscopy tools play indispensable role in nanoscience and nanotechnology research, as it provides rich information about the growth mechanism, chemical compositions, crystallography, and other important…

Deep learning has become an extremely effective tool for image classification and image restoration problems. Here, we apply deep learning to microscopy, and demonstrate how neural networks can exploit the chromatic dependence of the…

光学 · 物理学 2018-07-05 Eran Hershko* , Lucien E. Weiss* , Tomer Michaeli , Yoav Shechtman

Microfluidic devices offer numerous advantages in medical applications, including the capture of single cells in microwell-based platforms for genomic analysis. As the cost of sequencing decreases, the demand for high-throughput single-cell…

计算工程、金融与科学 · 计算机科学 2024-09-13 Xueying Zhao , Yan Chen , Yuefu Jiang , Amie Radenbaugh , Jamie Moskwa , Devon Jensen

Deep-learning algorithms enable precise image recognition based on high-dimensional hierarchical image features. Here, we report the development and implementation of a deep-learning-based image segmentation algorithm in an autonomous…

图像与视频处理 · 电气工程与系统科学 2020-03-26 Satoru Masubuchi , Eisuke Watanabe , Yuta Seo , Shota Okazaki , Takao Sasagawa , Kenji Watanabe , Takashi Taniguchi , Tomoki Machida
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