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Histological examination is crucial for cancer diagnosis, including hematoxylin and eosin (H&E) staining for mapping morphology and immunohistochemistry (IHC) staining for revealing chemical information. Recently developed two-color…

The importance of rapid and accurate histologic analysis of surgical tissue in the operating room has been recognized for over a century. Our standard-of-care intraoperative pathology workflow is based on light microscopy and H\&E…

Accurate intraoperative diagnosis is essential for providing safe and effective care during brain tumor surgery. Our standard-of-care diagnostic methods are time, resource, and labor intensive, which restricts access to optimal surgical…

Imaging Raman spectroscopy can be used to identify cancerous tissue. Traditionally, a step-by-step scanning of the sample is applied to generate a Raman image, which, however, is too slow for routine examination of patients. By transferring…

天体物理仪器与方法 · 物理学 2018-10-09 Elmar Schmälzlin , Benito Moralejo , Ingo Gersonde , Johannes Schleusener , Maxim E. Darvin , Gisela Thiede , Martin M. Roth

Histopathological images (HIs) are the gold standard for evaluating some types of tumors for cancer diagnosis. The analysis of such images is not only time and resource consuming, but also very challenging even for experienced pathologists,…

Histopathology, the current gold standard for cancer diagnosis, involves the manual examination of tissue samples after chemical staining, a time-consuming process requiring expert analysis. Raman spectroscopy is an alternative, stain-free…

Modern histopathology relies on the microscopic examination of thin tissue sections stained with histochemical techniques, typically using brightfield or fluorescence microscopy. However, the staining of samples can permanently alter their…

Histopathology plays a central role in diagnosis of many diseases including solid cancers. Efforts are underway to transform this subjective art form to an objective and quantitative science. Coherent Raman imaging (CRI), a label-free…

医学物理 · 物理学 2018-02-07 Marcus T Cicerone , Charles H. Camp

Histopathologic Images (HI) are the gold standard for evaluation of some tumors. However, the analysis of such images is challenging even for experienced pathologists, resulting in problems of inter and intra observer. Besides that, the…

计算机视觉与模式识别 · 计算机科学 2019-04-18 Jonathan de Matos , Alceu de Souza Britto , Luiz E. S. Oliveira , Alessandro L. Koerich

Background: Accurate diagnosis of skull base tumors is essential for providing personalized surgical treatment strategies. Intraoperative diagnosis can be challenging due to tumor diversity and lack of intraoperative pathology resources.…

Raman spectroscopy, a photonic modality based on the inelastic backscattering of coherent light, is a valuable asset to the intraoperative sensing space, offering non-ionizing potential and highly-specific molecular fingerprint-like…

图像与视频处理 · 电气工程与系统科学 2024-01-18 Ashutosh Raman , Ren A. Odion , Kent K. Yamamoto , Weston Ross , Tuan Vo-Dinh , Patrick J. Codd

For the Convolutional Neural Networks (CNNs) applied in the intelligent diagnosis of gastric cancer, existing methods mostly focus on individual characteristics or network frameworks without a policy to depict the integral information.…

计算机视觉与模式识别 · 计算机科学 2020-10-20 Changhao Sun , Chen Li , Jinghua Zhang , Muhammad Rahaman , Shiliang Ai , Hao Chen , Frank Kulwa , Yixin Li , Xiaoyan Li , Tao Jiang

We present a self-supervised algorithm for several classification tasks within hematoxylin and eosin (H&E) stained images of breast cancer. Our method is robust to stain variations inherent to the histology images acquisition process, which…

计算机视觉与模式识别 · 计算机科学 2023-09-08 Alexandre Tiard , Alex Wong , David Joon Ho , Yangchao Wu , Eliram Nof , Alvin C. Goh , Stefano Soatto , Saad Nadeem

Accurate and fast histological staining is crucial in histopathology, impacting diagnostic precision and reliability. Traditional staining methods are time-consuming and subjective, causing delays in diagnosis. Digital pathology plays a…

图像与视频处理 · 电气工程与系统科学 2023-07-06 Marian Boktor , James E. D. Tweel , Benjamin R. Ecclestone , Jennifer Ai Ye , Paul Fieguth , Parsin Haji Reza

We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique…

High-quality whole-slide scanning is expensive, complex, and time-consuming, thus limiting the acquisition and utilization of high-resolution histopathology images in daily clinical work. Deep learning-based single-image super-resolution…

图像与视频处理 · 电气工程与系统科学 2024-11-08 Minghong Duan , Linhao Qu , Zhiwei Yang , Manning Wang , Chenxi Zhang , Zhijian Song

In situ measurement of cellular metabolites is still a challenge in biology. Conventional methods, such as mass spectrometry or fluorescence microscopy, would either destruct the sample or introduce strong perturbations to the functions of…

定量方法 · 定量生物学 2019-12-19 Kai-Chih Huang , Junjie Li , Chi Zhang , Yuying Tan , Ji-Xin Cheng

Registration of serial whole-slide histopathological images (WSIs) is critical for enabling direct comparison across diverse stains and for preparing paired datasets in artificial intelligence (AI) workflows such as virtual staining and…

计算机视觉与模式识别 · 计算机科学 2025-09-04 Zeyu Liu , Shengwei Ding

Histopathological diagnoses of tumors in tissue biopsy after Hematoxylin and Eosin (H&E) staining is the gold standard for oncology care. H&E staining is slow and uses dyes, reagents and precious tissue samples that cannot be reused.…

Stimulated Raman scattering (SRS) microscopy offers great potential to surpass fluorescent-based approaches, owing to the sharp linewidth of Raman vibrations amenable to super-multiplex cell imaging, but currently lacks one crucial…

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