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Brillouin imaging is increasingly recognized to be a powerful technique that enables non-invasive measurement of the mechanical properties of cells and tissues on a microscopic scale. This provides an unprecedented tool for investigating…

生物物理 · 物理学 2020-01-22 Christine Poon , Joshua Chou , Michael Cortie , Irina Kabakova

Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous…

光学 · 物理学 2024-09-04 Carlo Bevilacqua , Robert Prevedel

Many problems in mechanobiology urgently require characterisation of the micromechanical properties of the fibrous proteins of cells and tissues. Brillouin light scattering has been proposed as a new optical elastography technique to meet…

Brillouin spectroscopy is a century-old technique that has recently received renewed interest, as modern instrumentation has transformed it into a powerful contactless and label-free probe of micromechanical properties for biomedical…

The biomechanical properties of cells and tissues play an important role in our fundamental understanding of the structures and functions of biological systems at both the cellular and subcellular levels. Recently, Brillouin microscopy,…

Brillouin spectroscopy and imaging are emerging techniques in analytical science, biophotonics and biomedicine. They are based on Brillouin light scattering from acoustic waves or phonons in the GHz range, providing a nondestructive…

生物物理 · 物理学 2021-10-12 Francesca Palombo , Daniele Fioretto

Brillouin microscopy is rapidly emerging as a powerful technique for imaging the mechanical properties of biological specimens in a label-free, non-contact manner. We present a standardized file format and open-source tools to facilitate…

Brillouin microscopy is an emerging technique for all-optical biomechanical imaging without the need for physical contact with the sample or for an external mechanical stimulus. However, Brillouin microscopy often retrieves a single,…

光学 · 物理学 2024-02-27 Roni Shaashoua , Tal Levy , Barak Rotblat , Alberto Bilenca

Brillouin imaging is an emerging optical elastography technique able to generate maps of the mechanical properties at microscale, with great potential in biophysical and biomedical fields. A key parameter is its spatial resolution, which is…

光学 · 物理学 2020-02-28 Silvia Caponi , Daniele Fioretto , Maurizio Mattarelli

In this study, Brillouin spectroscopy was employed to investigate the viscoelastic properties of melanoma cells in vitro. Using a custom-built confocal Brillouin microspectrometer, we obtained Brillouin shifts and full width at half maximum…

Characterizing the micromechanical properties of cells and extracellular matrices is critical in mechanobiology. To meet this need, Brillouin light scattering (BLS) has emerged as a noncontact, high-resolution elastography tool that probes…

Brillouin microscopy measures compressibility, but is being increasingly used to assess stiffness of cells and tissues. Using hydrogels with tunable properties, we demonstrate that Brillouin microscopy is insensitive to stiffness of…

Brillouin Microscopy is a novel label-free optical technique that enables the measurement of a material's mechanical properties at the sub-micron scale in a non-invasive and non-contact way; in the last few years, its applications in the…

Noncontact Brillouin spectroscopy is a purely optical and label-free method to retrieve fundamental material viscoelastic properties. Recently, the extension to a three-dimensional imaging modality has paved the way to novel exciting…

光学 · 物理学 2022-02-24 Giuseppe Antonacci , Kareem Elsayad , Dario Polli

Optical imaging with mechanical contrast is critical for material and biological discovery since it allows contactless light-radiation force-excitation within the sample, as opposed to traditional mechanical imaging. Whilst optical…

Noncontact label-free biomechanical imaging is a crucial tool for unraveling the mechanical properties of biological systems, which play critical roles in the fields of engineering, physics, biology and medicine; yet, it represents a…

生物物理 · 物理学 2020-09-29 Itay Remer , Netta Shemsesh , Anat Ben-Zvi , Alberto Bilenca

Brillouin microscopy is an emerging label-free imaging technique to assess local viscoelastic properties. Quantum-enhanced stimulated Brillouin scattering is demonstrated for the first time using low power continuous-wave lasers at 795~nm.…

光学 · 物理学 2024-09-16 Tian Li , Fu Li , Xinghua Liu , Vladislav V. Yakovlev , Girish S. Agarwal

Probing forces, deformations and generally speaking the mechanical properties of cells is the hallmark of mechanobiology. In the last two decades many techniques have been developed to this end that are largely based on deforming the cells…

Brillouin microscopy is an emerging optical technique for probing mechanical properties with submicron resolution, offering fully non-contact, label-free operation. Despite its unique capabilities, broader adoption has been limited by slow…

光学 · 物理学 2026-04-22 Mikolaj Pochylski
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