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The structural dynamics of macromolecules is important for most microbiological processes, from protein folding to the origins of neurodegenerative disorders. Noninvasive measurements of these dynamics are highly challenging. Recently,…

Absorption microscopy is a powerful technique, enabling the detection of single non- fluorescent molecules at room temperature. So far, the molecular absorption has been probed optically via the attenuation of a probing laser. The…

应用物理 · 物理学 2018-11-21 Miao-Hsuan Chien , Mario Brameshuber , Gerhard J. Schütz , Silvan Schmid

Astrometry measures shifts in a star's photocentre and can be used to detect reflex motion due to orbiting exoplanets. Brightness asymmetries (e.g. starspots) rotating in and out of view can also cause apparent motion of the photocenter,…

天体物理仪器与方法 · 物理学 2025-11-12 Jamila Taaki , Lia Corrales , Alfred Hero

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

定量方法 · 定量生物学 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

First discovered by Ernest Abbe in 1873, the resolution limit of a far-field microscope is considered determined by the numerical aperture and wavelength of light, approximately $\lambda$/2NA. With the advent of modern fluorescence…

光学 · 物理学 2023-08-02 Yilun Li , Fang Huang

We investigate the impact of rotational diffusion on the electrodynamic coupling of fluorescent dye molecules (oscillating electric dipoles) to a tunable planar metallic nanocavity. Fast rotational diffusion of the molecules leads to a…

Vorticity describes the spinning motion of a fluid, i.e., the tendency to rotate, at every point in a flow. The interest in performing accurate and localized measurements of vorticity reflects the fact that many of the quantities that…

光学 · 物理学 2015-07-30 Aniceto Belmonte Carmelo Rosales-Guzmán , Juan P. Torres

Several laser techniques have been suggested and demonstrated recently for preparing polarizable molecules in rapidly spinning states with a disc-like angular distribution. We consider motion of these spinning discs in inhomogeneous fields,…

化学物理 · 物理学 2015-05-20 Johannes Floss , Erez Gershnabel , Ilya Sh. Averbukh

Resonant frequency modulation spectroscopy has been previously used as a highly-sensitive method for measuring the output of cold atom interferometers. Using a detailed model that accounts for optical saturation, laser intensities and…

原子物理 · 物理学 2024-12-11 Ryan J. Thomas , Samuel R. Legge , Simon A. Haine , John D. Close

Since its invention in 1999, optical centrifuge has become a powerful tool for controlling molecular rotation and studying molecular dynamics and molecular properties at extreme levels of rotational excitation. The technique has been…

仪器与探测器 · 物理学 2020-05-20 Ian MacPhail-Bartley , Walter W. Wasserman , Alexander A. Milner , V. Milner

Active particle tracking microrheometers have the potential to perform accurate broad-band measurements of viscoelasticity within microscopic systems. Generally, their largest possible precision is limited by Brownian motion and low…

We consider optical properties of a gas of molecules that are brought to fast unidirectional spinning by a pulsed laser field. It is shown that a circularly polarized probe light passing through the medium inverts its polarization…

光学 · 物理学 2015-06-17 Uri Steinitz , Yehiam Prior , Ilya Sh. Averbukh

Single particle diffraction imaging experiments at free-electron lasers (FEL) have a great potential for structure determination of reproducible biological specimens that can not be crystallized. One of the challenges in processing the data…

生物物理 · 物理学 2013-10-16 O. M. Yefanov , I. A. Vartanyants

The coupling of nanostructures with emitters opens ways for the realization of man-made subwavelength light emitting elements. In this article, we investigate the modification of fluorescence when an emitter is placed close to a…

其他凝聚态物理 · 物理学 2009-11-13 S. Kuehn , G. Mori , M. Agio , V. Sandoghdar

We propose an approach to sensitively probe the chirality of molecules by measuring their coherent optical absorption spectra. It is shown that quantum dynamics of the cyclic three-level chiral molecules driven by appropriately-designed…

量子物理 · 物理学 2015-05-28 W. Z. Jia , L. F. Wei

By measuring the fundamental constants in astrophysical objects one can test basic physical principles as space-time invariance of physical laws along with probing the applicability limits of the standard model of particle physics. The…

天体物理仪器与方法 · 物理学 2016-03-07 S. A. Levshakov

Tracking single fluorescent molecules has offered resolution into dynamic molecular processes at the single-molecule level. This perspective traces the evolution of single-molecule tracking, highlighting key developments across various…

生物物理 · 物理学 2026-02-24 Lance W. Q. Xu , Steve Pressé

Localization of single fluorescent molecules is key for physicochemical and biophysical measurements such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy (SMLM). Recently a series of…

光学 · 物理学 2021-09-06 Luciano A. Masullo , Lucía F. Lopez , Fernando D. Stefani

The manipulation and metrology of light beams are pivotal for optical science and applications. In particular, achieving ultra-high precision in the measurement of light beam rotations has been a long-standing challenge. Instead of…

量子物理 · 物理学 2025-10-13 Binke Xia , Jingzheng Huang , Hongjing Li , Zhongyuan Luo , Guihua Zeng

We report on the direct measurements of fluid flow vorticity using a spatially shaped beam with a superposition of Laguerre-Gaussian modes that reports on the rotational Doppler shift from microparticles intersecting the beam focus.…

光学 · 物理学 2016-06-29 A. Ryabtsev , S. Pouya , A. Safaripour , M. Koochesfahani , M. Dantus