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The blind structured illumination microscopy (SIM) strategy proposed in (Mudry et al., 1992) is fully re-founded in this paper, unveiling the central role of the sparsity of the illumination patterns in the mechanism that drives…

数据分析、统计与概率 · 物理学 2017-04-26 Simon Labouesse , Awoke Negash , Jérôme Idier , Sébastien Bourguignon , Thomas Mangeat , Penghuan Liu , Anne Sentenac , Marc Allain

Structured illumination microscopy (SIM) improves resolution by down-modulating high-frequency information of an object to fit within the passband of the optical system. Generally, the reconstruction process requires prior knowledge of the…

计算机视觉与模式识别 · 计算机科学 2017-01-13 Li-Hao Yeh , Lei Tian , Laura Waller

Structured illumination microscopy (SIM) is a very important super-resolution microscopy technique, which provides high speed super-resolution with about two-fold spatial resolution enhancement. Several attempts aimed at improving the…

计算机视觉与模式识别 · 计算机科学 2016-02-23 Amit Lal , Chunyan Shan , Peng Xi

In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than…

数据分析、统计与概率 · 物理学 2016-11-18 S. Labouesse , M. Allain , J. Idier , S. Bourguignon , A. Negash , P. Liu , A. Sentenac

Structured illumination microscopy (SIM) can double the resolution beyond the light diffraction limit, but it comes at the cost of multiple camera exposures and the heavy computation burden of multiple Fourier transforms. In this paper, we…

图像与视频处理 · 电气工程与系统科学 2021-07-14 Qinnan Zhang , En Bo , Jiawei Chen , Jiaosheng Li , Heming Jiang , Xiaoxu Lu , Liyun Zhong , Jindong Tian

By exploiting the nonlinear responses of the fluorescent probes, the spatial resolution of structured illumination microscopy(SIM) can be further increased. However, due to the complex reconstruction process, the traditional reconstruction…

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

光学 · 物理学 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Structured illumination microscopy (SIM) is one of the most versatile super-resolution techniques. Yet, its application to live imaging has been so far mainly limited to fluorescent and stationary specimens. Here, we present advancements in…

Structured Illumination Microscopy is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep…

图像与视频处理 · 电气工程与系统科学 2021-02-19 Miguel Boland , Edward A. K. Cohen , Seth Flaxman , Mark A. A. Neil

Structured illumination microscopy (SIM) is a pivotal technique for dynamic subcellular imaging in live cells. Conventional SIM reconstruction algorithms depend on accurately estimating the illumination pattern and can introduce artefacts…

光学 · 物理学 2025-03-17 Long-Kun Shan , Ze-Hao Wang , Tong-Tian Weng , Xiang-Dong Chen , Fang-Wen Sun

Structured illumination microscopy (SIM) has become an important technique for optical super-resolution imaging because it allows a doubling of image resolution at speeds compatible for live-cell imaging. However, the reconstruction of SIM…

图像与视频处理 · 电气工程与系统科学 2020-03-26 Charles N. Christensen , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Structured illumination microscopy (SIM) is a wide-field super-resolution technique normally limited to roughly twice the diffraction-limited resolution ($\approx 100$--$200$~nm). Surpassing this bound is a classic ill-posed inverse…

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…

光学 · 物理学 2022-03-09 James D. Manton

Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…

光学 · 物理学 2024-10-16 Doron Shterman , Guy Bartal

Diffraction-limited imaging in epi-fluorescence microscopy remains a challenge when sample aberrations are present or when the region of interest rests deep within an inhomogeneous medium. Adaptive optics is an attractive solution albeit…

Structured Illumination Microscopy (SIM) is an imaging technique for achieving both super-resolution (SR) and optical sectioning (OS) in wide-field microscopy. It consists in illuminating the sample with periodic patterns at different…

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

光学 · 物理学 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Structured Illumination Microscopy (SIM) overcomes the optical diffraction limit by folding high-frequency components into the baseband of the optical system, where they can be extracted and then repositioned to their original location in…

光学 · 物理学 2024-11-18 Doron Shterman , Guy Bartal

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

图像与视频处理 · 电气工程与系统科学 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Structured illumination microscopy (SIM) enables live cell, super-resolution imaging at high speeds. SIM uses sophisticated optical systems to generate pre-determined excitation light patterns, and reconstruction algorithms to enhance the…

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