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The mechanical properties of biological fluids can serve as early indicators of disease, offering valuable insights into complex physiological and pathological processes. However, the existing technologies can hardly support high throughput…

光学 · 物理学 2025-09-18 Zhonghao Li , Zhihan Cai , Chaoyang Gong

Droplet microfluidics offers a versatile platform for analyzing liquid samples. Despite its potential, there is a lack of techniques that allow to reliably probe individual circulating droplets. The prospect of combining droplet…

定量方法 · 定量生物学 2024-03-08 Marc Sulliger , Jaime Ortega Arroyo , Romain Quidant

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

图像与视频处理 · 电气工程与系统科学 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

This work demonstrates a multi-lens microscopic imaging system that overlaps multiple independent fields of view on a single sensor for high-efficiency automated specimen analysis. Automatic detection, classification and counting of various…

计算机视觉与模式识别 · 计算机科学 2022-01-19 Xing Yao , Vinayak Pathak , Haoran Xi , Amey Chaware , Colin Cooke , Kanghyun Kim , Shiqi Xu , Yuting Li , Timothy Dunn , Pavan Chandra Konda , Kevin C. Zhou , Roarke Horstmeyer

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

图像与视频处理 · 电气工程与系统科学 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Intracellular lasers are emerging as powerful biosensors for multiplexed tracking and precision sensing of cells and their microenvironment. This sensing capacity is enabled by quantifying their narrow-linewidth emission spectra, which is…

光学 · 物理学 2023-10-31 Soraya Caixeiro , Philip Wijesinghe , Kishan Dholakia , Malte C. Gather

Artificial nanostructures with ultrafine and deep-subwavelength feature sizes have emerged as a paradigm-shifting platform to advanced light field management, becoming a key building block for high-performance integrated optoelectronics and…

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

仪器与探测器 · 物理学 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

Scattering-based single-particle tracking (S-SPT) has opened new avenues for highly sensitive label-free detection and characterization of nanoscopic objects, making it particularly attractive for various analytical applications. However, a…

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

物理教育 · 物理学 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

Mid-infrared absorption spectroscopy is a well-established technique for non-destructive quantitative molecular analysis. Waveguide-integrated sensors provide a particularly compact solution operating with reduced sample volumes while…

光学 · 物理学 2024-08-16 Antonia Torres-Cubillo , Andrea Teuber , Robert Halir , Boris Mizaikoff

Label-free spectromicroscopy methods offer the capability to examine complex cellular phenomena. Electron and X-ray-based spectromicroscopy methods, though powerful, have been hard to implement with hydrated objects due to the vacuum…

High-throughput biological imaging is often constrained by a trade-off between acquisition speed and image quality. Fast imaging modalities, such as wide-field fluorescence microscopy, enable large-scale data acquisition but suffer from…

图像与视频处理 · 电气工程与系统科学 2026-04-20 Dominik Panek , Carina Rząca , Maksymilian Szczypior , Joanna Sorysz , Krzysztof Misztal , Zbigniew Baster , Zenon Rajfur

Size and shape are critical discriminators between molecular species and states. We describe a micro-chip based high-throughput imaging approach offering rapid and precise determination of molecular properties under native solution…

Wireless capsule endoscopy is a medical procedure used to visualize the entire gastrointestinal tract and to diagnose intestinal conditions, such as polyps or bleeding. Current analyses are performed by manually inspecting nearly each one…

图像与视频处理 · 电气工程与系统科学 2020-10-05 Pablo Laiz , Jordi Vitrià , Hagen Wenzek , Carolina Malagelada , Fernando Azpiroz , Santi Seguí

Many essential cellular functions depend on the viscoelastic properties of the cytoplasm. While techniques such as optical tweezers and atomic force microscopy can measure these properties, their reliance on localized probes prevents…

Microfluidic droplet screens serve as an innovative platform for high-throughput biotechnology, enabling significant advancements in discovery, product optimization, and analysis. This review sheds light on the emerging trend of interaction…

定量方法 · 定量生物学 2023-09-11 Carolus Vitalis , Tobias Wenzel

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

Lensless on-chip microscopy has shown great potential for biomedical imaging due to its large-area and high-throughput imaging capabilities. By combining the pixel super-resolution (PSR) technique, it can improve the resolution beyond the…

图像与视频处理 · 电气工程与系统科学 2024-09-06 Xuyang Chang , Shaowei Jiang , Yongcun Hu , Liheng Bian

Lensless imaging is an elegant approach to high-resolution microscopy, which is rapidly gaining popularity in applications where imaging optics are problematic. However, current lensless imaging methods require objects to be placed within a…

光学 · 物理学 2014-06-16 Stefan Witte , Vasco T. Tenner , Daniël W. E. Noom , Kjeld S. E. Eikema
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