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We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off the shelf components, such as a light emitting diode…

光学 · 物理学 2016-02-17 Juan Miguel Pérez , Marc Jofre , Pedro Martínez , Valerio Pruneri

Flow cytometry mainly used for detecting the characteristics of a number of biochemical substances based on the expression of specific markers in cells. It is particularly useful for detecting membrane surface receptors, antigens, ions, or…

机器学习 · 计算机科学 2023-03-17 Yanhua Xu

Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

定量方法 · 定量生物学 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

In this article we present a new diagnostic approach utilizing flow-cytometry to study compounds of nanoparticle samples in solution by analysis of their scattering patterns retrieved from the cytometric measurements. As a specific…

Immunoaffinity-based liquid biopsies of circulating tumor cells (CTCs) hold great promise for cancer management, but typically suffer from low throughput, relative complexity and post-processing limitations. Here we address these issues…

定量方法 · 定量生物学 2026-04-29 Jing Yan , Jie Wang , Robert Dallmann , Renquan Lu , Jérôme Charmet

Flow cytometry is a valuable technique that measures the optical properties of particles at a single-cell resolution. When deployed in the ocean, flow cytometry allows oceanographers to study different types of photosynthetic microbes…

统计方法学 · 统计学 2026-04-02 Ethan Pawl , François Ribalet , Paul A. Parker , Sangwon Hyun

Flow cytometry (FCM) is an analytic technique that is capable of detecting and recording the emission of fluorescence and light scattering of cells or particles (that are collectively called "events") in a population. A typical FCM…

定量方法 · 定量生物学 2020-11-20 Nathan Wong , Daehwan Kim , Zachery Robinson , Connie Huang , Irina M. Conboy

Flow cytometry is a technique that measures multiple fluorescence and light scatter-associated parameters from individual cells as they flow a single file through an excitation light source. These cells are labeled with antibodies to detect…

The ocean is filled with microscopic microalgae called phytoplankton, which together are responsible for as much photosynthesis as all plants on land combined. Our ability to predict their response to the warming ocean relies on…

应用统计 · 统计学 2022-08-05 Sangwon Hyun , Mattias Rolf Cape , Francois Ribalet , Jacob Bien

Bioclogging, the clogging of pores with living particles, is a complex process that involves various coupled mechanisms such as hydrodynamics and particle properties. This article explores bioclogging at the microscale level. At this scale,…

We present cytometric classification of live healthy and cancer cells by using the spatial morphological and textural information found in the label-free quantitative phase images of the cells. We compare both healthy cells to primary tumor…

The Auto-ICell system, a novel, and cost-effective integrated droplet microfluidic system, is introduced for real-time analysis of single-cell morphology and apoptosis. This system integrates a 3D-printed microfluidic chip with image…

图像与视频处理 · 电气工程与系统科学 2023-11-07 Yuanyuan Wei , Meiai Lin , Shanhang Luo , Syed Muhammad Tariq Abbasi , Liwei Tan , Guangyao Cheng , Bijie Bai , Yi-Ping Ho , Scott Wu Yuan , Ho-Pui Ho

We report a field-portable and cost-effective imaging flow cytometer that uses deep learning to accurately detect Giardia lamblia cysts in water samples at a volumetric throughput of 100 mL/h. This flow cytometer uses lensfree color…

Flow cytometry is a widespread and powerful technique, whose resolution is determined by its capacity to accurately distinguish fluorescently positive populations from negative ones. However, most informative results are discarded while…

Flow cytometry is a powerful quantitative assay supporting high-throughput collection of single-cell data with a high dynamic range. For flow cytometry to yield reproducible data with a quantitative relationship to the underlying biology,…

定量方法 · 定量生物学 2023-05-16 Jacob Beal

Phytoplankton are microscopic algae responsible for roughly half of the world's photosynthesis that play a critical role in global carbon cycles and oxygen production, and measuring the abundance of their subtypes across a wide range of…

统计方法学 · 统计学 2025-10-08 Farhad de Sousa , François Ribalet , Jacob Bien

Ocean microbes are critical to both ocean ecosystems and the global climate. Flow cytometry, which measures cell optical properties in fluid samples, is routinely used in oceanographic research. Despite decades of accumulated data,…

统计方法学 · 统计学 2025-04-17 Sangwon Hyun , Tim Coleman , Francois Ribalet , Jacob Bien

The intra-cluster light (ICL) in observations is usually identified through the surface brightness limit method. In this paper, for the first time we produce the mock images of galaxy groups and clusters using a cosmological hydro-…

星系天体物理 · 物理学 2018-06-06 Lin Tang , WeiPeng Lin , Weiguang Cui , Xi Kang , Yang Wang , E. Contini , Yu Yu

Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…

定量方法 · 定量生物学 2025-11-03 Surajit Das , Pavel Zun

Flow cytometry is a cornerstone technique in medical and biological research, providing crucial information about cell size and granularity through forward scatter (FSC) and side scatter (SSC) signals. Despite its widespread use, the…

光学 · 物理学 2024-08-29 Jaepil Jo , Herve Hugonnet , Mahn Jae Lee , YongKeun Park
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