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The structural reorganization of the actin cytoskeleton is facilitated through the action of motor proteins that crosslink the actin filaments and transport them relative to each other. Here, we present a combined experimental-computational…

生物物理 · 物理学 2015-06-04 Daniel Gordon , Anne Bernheim-Groswasser , Chen Keasar , Oded Farago

The actin cytoskeleton is remarkably adaptable and multifunctional. It often organizes into nematic bundles such as contractile rings or stress fibers. However, how a uniform and isotropic actin gel self-organizes into dense nematic bundles…

Living cells dynamically modulate the local morphologies of their actin cytoskeletons to perform biological functions, including force transduction, intracellular transport, and cell division. A major challenge is to understand how diverse…

生物物理 · 物理学 2018-12-07 Simon L. Freedman , Glen M. Hocky , Shiladitya Banerjee , Aaron R. Dinner

Cortical actin networks are highly dynamic and play critical roles in shaping the mechanical properties of cells. The actin cytoskeleton undergoes significant reorganization over the course of the cell cycle, when cortical actin transitions…

定量方法 · 定量生物学 2022-07-22 Maria-Veronica Ciocanel , Aravind Chandrasekaran , Carli Mager , Qin Ni , Garegin Papoian , Adriana Dawes

Cells and tissues exert forces and can actively change shape. This strikingly autonomous behavior is powered by the cytoskeleton, which includes an active gel of actin filaments, crosslinks, and myosin molecular motors. Although individual…

软凝聚态物质 · 物理学 2017-07-26 José Alvarado , Misha Sheinman , Abhinav Sharma , Fred C. MacKintosh , Gijsje H. Koenderink

Actin filaments, crosslinkers and myosin molecular motors form contractile networks. For instance, the cell cortex is a thin network below the cell membrane ; contraction of the cell cortex allows cells to round up during cell division.…

The actin cytoskeleton is a key component in the machinery of eukaryotic cells, and it selfassembles out of equilibrium into a wide variety of biologically crucial structures. While the molecular mechanisms involved are well characterized,…

软凝聚态物质 · 物理学 2017-01-04 Giulia Foffano , Nicolas Levernier , Martin Lenz

The actin cytoskeleton in living cells generates forces in conjunction with myosin motor proteins to directly and indirectly drive essential cellular processes. The semiflexible filaments of the cytoskeleton can respond nonlinearly to the…

软凝聚态物质 · 物理学 2019-12-19 D. Mizuno , C. Tardin , C. F. Schmidt

Cells and tissues have the remarkable ability to actively generate the forces required to change their shape. This active mechanical behavior is largely mediated by the actin cytoskeleton, a crosslinked network of actin filaments that is…

软凝聚态物质 · 物理学 2018-12-18 Jose Alvarado , Luca Cipelletti , Gijsje Koenderink

The composite cytoskeleton, comprising interacting networks of semiflexible actin and rigid microtubules, actively generates forces and restructures using motor proteins such as myosins to enable key mechanical processes including cell…

Structure formation and constant reorganization of the actin cytoskeleton are key requirements for the function of living cells. Here we show that a minimal reconstituted system consisting of actin filaments, crosslinking molecules and…

生物物理 · 物理学 2015-05-27 Simone Köhler , Volker Schaller , Andreas R. Bausch

Living cells move thanks to assemblies of actin filaments and myosin motors that range from very organized striated muscle tissue to disordered intracellular bundles. The mechanisms powering these disordered structures are debated, and all…

生物物理 · 物理学 2017-09-26 Martin Lenz

Contractile forces are essential for many developmental processes involving cell shape change and tissue deformation. Recent experiments on reconstituted actomyosin networks, the major component of the contractile machinery, have shown that…

生物物理 · 物理学 2012-05-31 Shenshen Wang , Peter G. Wolynes

Self organization mechanisms are essential for the cytoskeleton to adapt to the requirements of living cells. They rely on the intricate interplay of cytoskeletal filaments, crosslinking proteins and molecular motors. Here we present an in…

生物物理 · 物理学 2015-05-28 Simone Köhler , Volker Schaller , Andreas R. Bausch

Living systems often exhibit internal driving: active, molecular processes drive nonequilibrium phenomena such as metabolism or migration. Active gels constitute a fascinating class of internally driven matter, where molecular motors exert…

The cytoskeleton is a model active matter system that controls diverse cellular processes from division to motility. While both active actomyosin dynamics and actin-microtubule interactions are key to the cytoskeleton's versatility and…

The mechanics of cells is strongly affected by molecular motors that generate forces in the cellular cytoskeleton. We develop a model for cytoskeletal networks driven out of equilibrium by molecular motors exerting transient contractile…

软凝聚态物质 · 物理学 2009-11-13 Fred C. MacKintosh , Alex J. Levine

Actin is one of the most studied cytoskeleton proteins showing a very rich span of structures. It can self-assemble actively into dynamical structures that govern the mechanical properties of the cell, its motility and its division.…

软凝聚态物质 · 物理学 2020-03-17 Maayan Levin , Raya Sorkin , David Pine , Rony Granek , Anne Bernheim-Groswasser , Yael Roichman

In this work, we study in detail the distribution of stochastic forces generated by the molecular motors activity, in the actin cortex of pre-muscular cells. By combining active and passive rheology experiments, performed on the same…

生物物理 · 物理学 2019-12-10 P. Bohec , J. Tailleur , F. van Wijland , A. Richert , F. Gallet

There is compelling evidence that substrate stiffness affects cell adhesion as well as cytoskeleton organization and contractile activity. This work was designed to study the cytoskeletal contractile activity of cells plated on microposts…

细胞行为 · 定量生物学 2019-09-13 Ian Manifacier , Kevin Beussman , Sangyoon Han , Nathan Sniadecki , Imad About , Jean-Louis Milan
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