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This paper presents an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high-resolution digital recording,…

光学 · 物理学 2007-05-23 Sergey A. Alexandrov , P. Meredith , T. J. McIntyre , A. V. Zvyagin

Lensless digital holographic microscopy (LDHM) offers very large field-of-view label-free imaging crucial, e.g., in high-throughput particle tracking and biomedical examination of cells and tissues. Compact layouts promote point-of-case and…

光学 · 物理学 2023-10-24 Piotr Arcab , Mikolaj Rogalski , Maciej Trusiak

Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy…

光学 · 物理学 2025-01-20 Jose Angel Picazo-Bueno , Maciej Trusiak , Javier Garcia , Vicente Mico

Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

光学 · 物理学 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…

Lensless digital holographic microscopy (LDHM) relies on interference between an unscattered reference wave and a weakly scattered object wave - an assumption that rapidly fails in turbid samples under multiple scattering. To overcome this…

The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…

光学 · 物理学 2025-01-22 Jaromír Běhal , Miroslav Ježek

This industrial Ph.D. project, carried out in collaboration between Radiometer Medical ApS and SDU Centre for Photonics Engineering at the University of Southern Denmark, explored the use of digital holographic microscopy (DHM) for the…

光学 · 物理学 2026-02-10 Andreas Erik Gejl Madsen

Fourier ptychographic microscopy (FPM) is a recently developed imaging modality that uses angularly varying illumination to extend a system performance beyond the limit defined by its optical elements. The FPM technique applies a novel…

光学 · 物理学 2014-05-02 Xiaoze Ou , Roarke Horstmeyer , Changhuei Yang , Guoan Zheng

In this chapter, we introduce digital holographic microscopy (DHM) as a marker-free method to determine the refractive index of single, spherical cells in suspension. The refractive index is a conclusive measure in a biological context.…

In recent years, interest in expanding from 2D to 3D systems has grown in the magnetism community, from exploring new geometries to broadening the knowledge on the magnetic textures present in thick samples, and with this arise the need for…

Off-axis digital holographic microscopy is a high-throughput, label-free imaging technology that provides three-dimensional, high-resolution information about samples, particularly useful in large-scale cellular imaging. However, the…

We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…

Off-axis X-ray holography is a lensless imaging technique that allows unambiguous retrieval of an object's exit-wave function with high fidelity. It has been used with great success to study nanoscale phenomena and spatio-temporal dynamics…

光学 · 物理学 2021-05-11 Kahraman Keskinbora , Abraham Levitan , Gisela Schütz , Riccardo Comin

Quantitative phase imaging (QPI) enables label-free, high-contrast visualization of transparent specimens, but its common implementation in off-axis digital holographic microscopy (DHM) requires a separate reference beam, which increases…

A lensless digital holography enables wide-field microscopic imaging without the limitations imposed by optical lens performance. However, conventional holographic imaging often relies on magnifying optical systems to compensate for the low…

光学 · 物理学 2025-06-24 Byung Gyu Chae

Phase retrieval is the inverse problem of recovering a signal from magnitude-only Fourier measurements, and underlies numerous imaging modalities, such as Coherent Diffraction Imaging (CDI). A variant of this setup, known as holography,…

机器学习 · 计算机科学 2021-04-22 Hannah Lawrence , David A. Barmherzig , Henry Li , Michael Eickenberg , Marylou Gabrié

Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging. Recovery of the missing phase information of a hologram is an important step in holographic image reconstruction. Here we demonstrate…

图像与视频处理 · 电气工程与系统科学 2021-05-28 Luzhe Huang , Tairan Liu , Xilin Yang , Yi Luo , Yair Rivenson , Aydogan Ozcan

Digital holography numerically restores three-dimensional image information using optically captured diffractive waves. The required bandwidth is larger than that of hologram pixel at a closer distance in the Fresnel diffraction regime,…

光学 · 物理学 2024-01-12 Byung Gyu Chae

Holographic Tomography (HT) or Optical Diffraction Tomography (ODT) provides slice-by-slice information about the refractive index (RI) of three-dimensional (3D) samples and is emerging as an important label-free imaging modality for Life…

图像与视频处理 · 电气工程与系统科学 2025-04-22 N Goyal , K Khare
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