相关论文: Cell shape identification using digital holographi…
Some microalgae store large amounts of neutral lipids inside lipid droplets. Since these lipids can be used to produce biodiesel in a sustainable way, research is developing on fast non-destructive methods to quantify and monitor the amount…
We present Defocusing Microscopy (DM), a bright-field optical microscopy technique able to perform total 3D imaging of transparent objects. By total 3D imaging we mean the determination of the actual shapes of the upper and lower surfaces…
Digital holographic microscopy is a fast three-dimensional (3D) imaging tool with many applications in soft matter physics. Recent studies have shown that electromagnetic scattering solutions can be fit to digital holograms to obtain the 3D…
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured illumination microscopy (SIM).…
We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample…
This work demonstrates an application of near field indirect microwave holography for the detection of malignant tissues in the human breast in an effective way. The holograms are recorded by two directive antennas aligned along each…
Holography exploits the interference of light fields to obtain a systematic reconstruction of the light fields wavefronts. Classical holography techniques have been very successful in diverse areas such as microscopy, manufacturing…
Although cell shape can reflect the mechanical and biochemical properties of the cell and its environment, quantification of 3D cell shapes within 3D tissues remains difficult, typically requiring digital reconstruction from a stack of 2D…
We introduce a novel framework that incorporates multiple scattering for large-scale 3D particle-localization using single-shot in-line holography. Traditional holographic techniques rely on single-scattering models which become inaccurate…
We propose a new learning-based approach for 3D particle field imaging using holography. Our approach uses a U-net architecture incorporating residual connections, Swish activation, hologram preprocessing, and transfer learning to cope with…
Using optical speckle scanning microscopy [1], we demonstrate that clear images of multiple cells can be obtained through biological scattering tissue, with subcellular resolution and good image quality, as long as the size of the imaging…
We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…
Biological cell imaging has become one of the most crucial research interests due to its wide-ranging applications in biomedical and microbiology studies. However, three-dimensional (3D) imaging of biological cells remains critically…
We propose a holographic microscopy method based on single-pixel imaging technology (HM-SPI). We used a holographic microscopy method based on in-line Gabor holography. In single-pixel imaging technology, cyclic binary masks and…
Digital holographic microscopy based on Gabor in-line holography is a well-known method to reconstruct both the amplitude and phase of small objects. To reconstruct the image of an object from its hologram, obtained under illumination by…
We introduce a novel reflection-mode diffraction tomography technique that enables simultaneous recovery of forward and backward scattering information for high-resolution 3D refractive index reconstruction. Our technique works by imaging a…
In digital holography, the coherent scattered light fields can be reconstructed volumetrically. By refocusing the fields to the sample planes, absorption and phase-shift profiles of sparsely distributed samples can be simultaneously…
In this work we propose an approach to select the classification method and features, based on the state-of-the-art, with best performance for diagnostic support through peripheral blood smear images of red blood cells. In our case we used…
We present a method based on a time domain simulation of wave propagation that allows studying the optical response of a broad range of dielectric photonic structures. This method is particularly suitable for dealing with complex biological…
Digital holographic microscopy is an emerging potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micron-scale objects however requires a…