相关论文: Neuronal micro-culture engineering by microchannel…
In vitro neuronal culture is an important research platform in cellular and network neuroscience. However, neurons cultured on a homogeneous scaffold form dense, randomly connected networks and display excessively synchronized activity;…
Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined…
The spontaneous activity pattern of cortical neurons in dissociated culture is characterized by burst firing that is highly synchronized among a wide population of cells. The degree of synchrony, however, is excessively higher than that in…
Unlike other tissue types, like epithelial tissue, which consist of cells with a much more homogeneous structure and function, the nervous tissue spans in a complex multilayer environment whose topographical features display a large…
Two major initiatives to accelerate research in the brain sciences have focused attention on developing a new generation of scientific instruments for neuroscience. These instruments will be used to record static (structural) and dynamic…
Cells maintain a stable size as they grow and divide. Inspired by the available experimental data, most proposed models for size homeostasis assume size control mechanisms that act on a timescale of one generation. Such mechanisms lead to…
Neuronal growth cones are the most sensitive amongst eukaryotic cells in responding to directional chemical cues. Although a dynamic microtubule cytoskeleton has been shown to be essential for growth cone turning, the precise nature of…
Chronic alcoholism is known to alter morphology of hippocampal, an important region of cognitive function in the brain. We performed quantification of nanoscale structural alterations in nuclei of hippocampal neuron cells due to chronic…
Understanding the nature of the changes exhibited by evolving neuronal dynamics from high-dimensional activity data is essential for advancing neuroscience, particularly in the study of neuronal network development and the pathophysiology…
Polydimethylsiloxane (PDMS) microfluidic devices have become a standard tool for engineering cells and multicellular networks in vitro. However, the reservoirs, or through-holes where cells access the devices, are usually fabricated…
Intracellular processes triggered by neural activity include changes in ionic concentrations, protein release, and synaptic vesicle cycling. These processes play significant roles in neurological disorders. The beneficial effects of brain…
Various microorganisms and some mammalian cells are able to swim in viscous fluids by performing nonreciprocal body deformations, such as rotating attached flagella or by distorting their entire body. In order to perform chemotaxis, i.e. to…
In this paper we present a simple microscopic stochastic model describing short term plasticity within a large homogeneous network of interacting neurons. Each neuron is represented by its membrane potential and by the residual calcium…
The microtubule cytoskeleton is responsible for sustained, long-range intracellular transport of mRNAs, proteins, and organelles in neurons. Neuronal microtubules must be stable enough to ensure reliable transport, but they also undergo…
Detailed knowledge of mechanical parameters such as cell elasticity, stiffness of the growth substrate, or traction stresses generated during axonal extensions is essential for understanding the mechanisms that control neuronal growth. Here…
Single cell and regularly cells docking inside the microstructure of microfluidic systems are advantageous in different analyses of single cells exposed to equal drug concentration and mechanical stimulus. In this study, we investigated…
We investigate the transport dynamics of elongated microparticles in microchannel flows. While smooth-walled channels preserve the dependence of particle trajectories on initial orientation and lateral position, we show that introducing…
The electrical properties of extracellular space around neurons are important to understand the genesis of extracellular potentials, as well as for localizing neuronal activity from extracellular recordings. However, the exact nature of…
Learning and memory relies on synapses changing their strengths in response to neural activity. However there is a substantial gap between the timescales of neural electrical dynamics (1-100 ms) and organism behaviour during learning…
Neural systems can be modeled as networks of functionally connected neural elements. The resulting network can be analyzed using mathematical tools from network science and graph theory to quantify the system's topological organization and…