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Fluctuations in the abundance of molecules in the living cell may affect its growth and well being. For regulatory molecules (e.g., signaling proteins or transcription factors), fluctuations in their expression can affect the levels of…

分子网络 · 定量生物学 2009-11-13 Erel Levine , Terence Hwa

Quantifying the irreversibility and dissipation of non-equilibrium processes is crucial to understanding their behavior, assessing their possible capabilities, and characterizing their efficiency. We introduce a physical quantity that…

统计力学 · 物理学 2025-11-13 Alvaro Lanza , Inés Martínez-Martín , Rafael Tapia-Rojo , Stefano Bo

In single-molecule force spectroscopy experiments, the dependence of the mean unfolding force on the loading rate is used for obtaining information about the energetic and dynamic properties of the system under study. However, it is crucial…

生物物理 · 物理学 2020-03-10 Rafayel Petrosyan

Despite more than 100 years of study, it is unclear if the movement of proteins inside the cell is best described as a mosh pit or an exquisitely choreographed dance. Recent studies suggest the latter. Local interactions induce molecular…

定量方法 · 定量生物学 2023-08-25 James A. Galbraith , Catherine G. Galbraith

Density dependence is important in the ecology and evolution of microbial and cancer cells. Typically, we can only measure net growth rates, but the underlying density-dependent mechanisms that give rise to the observed dynamics can…

种群与进化 · 定量生物学 2025-06-04 Linh Huynh , Jacob G. Scott , Peter J. Thomas

When analyzing the individual positional dynamics of an ensemble of moving objects, the extracted parameters that characterize the motion of individual objects, such as the mean-squared instantaneous velocity or the diffusivity, exhibit a…

Single molecule manipulation techniques reveal that the mechanical resistance of a protein depends on the direction of the applied force. Using a lattice model of polymers, we show that changing the pulling direction leads to different…

统计力学 · 物理学 2009-11-13 Sanjay Kumar , Debaprasad Giri

The effect of disorder in the energetic heights and in the physical locations of fence barriers encountered by transmembrane molecules such as proteins and lipids in their motion in cell membranes is studied theoretically. The investigation…

生物物理 · 物理学 2009-11-13 Z. Kalay , P. E. Parris , V. M. Kenkre

Dynamic force spectroscopy of single molecules is described by a model which predicts a distribution of rupture forces, the corresponding mean rupture force and variance, all amenable to experimental tests. The distribution has a pronounced…

软凝聚态物质 · 物理学 2007-05-23 O. K. Dudko , A. E. Filippov , J. Klafter , M. Urbakh

In biological materials, strong binding despite an applied load force is often based on clusters of dynamic bonds that share the load. Different macroscopic behaviors have been described depending on whether the load is shared locally or…

生物物理 · 物理学 2025-09-11 Yannick Lüdemann , Stefan Klumpp , Komal Bhattacharyya

Information transmission in cells occurs quite accurately even when concentration changes are "read" by individual target molecules. In this Letter we study molecule number fluctuations when molecules diffuse and react. We show that, for…

生物物理 · 物理学 2014-12-17 Emiliano Pérez Ipiña , Silvina Ponce Dawson

Even in the steady-state, the number of biomolecules in living cells fluctuates dynamically; and the frequency spectrum of this chemical fluctuation carries valuable information about the mechanism and the dynamics of the intracellular…

生物物理 · 物理学 2018-08-21 Sanggeun Song , Gil-Suk Yang , Seong Jun Park , Ji-Hyun Kim , Jaeyoung Sung

Living cells exhibit an important out-of-equilibrium mechanical activity, mainly due to the forces generated by molecular motors. These motor proteins, acting individually or collectively on the cytoskeleton, contribute to the violation of…

生物物理 · 物理学 2009-01-21 Francois Gallet , Delphine Arcizet , Pierre Bohec , Alain Richert

Curvatures in the most probable rupture force ($f^*$) versus log-loading rate ($\log{r_f}$) observed in dynamic force spectroscopy (DFS) on biomolecular complexes are interpreted using a one-dimensional free energy profile with multiple…

生物物理 · 物理学 2015-06-04 Changbong Hyeon , D. Thirumalai

Motor-driven cargo transport is a complex phenomenon where multiple motor proteins attached on to a cargo engage in pulling activity, often leading to tug-of-war, displaying bidirectional motion. However, most mathematical and computational…

生物物理 · 物理学 2016-09-09 Deepak Bhat , Manoj Gopalakrishnan

One of the most challenging problems in microbiology is to understand how a small fraction of microbes that resists killing by antibiotics can emerge in a population of genetically identical cells, the phenomenon known as persistence or…

亚细胞过程 · 定量生物学 2015-06-17 Andrea Rocco , Andrzej M. Kierzek , Johnjoe McFadden

Motivated by a number of recent experimental and computational studies of the dynamics of fluids plunged in quenched-disordered external fields, we report on a theoretical investigation of this topic within the framework of the…

软凝聚态物质 · 物理学 2018-10-05 Thomas Konincks , Vincent Krakoviack

Single-molecule force spectroscopy has proven to be a powerful tool for studying the kinetic behavior of biomolecules. Through application of an external force, conformational states with small or transient populations can be stabilized,…

Over the past decade, advances in super-resolution microscopy and particle-based modeling have driven an intense interest in investigating spatial heterogeneity at the level of single molecules in cells. Remarkably, it is becoming clear…

分子网络 · 定量生物学 2013-11-19 Andrew Mugler , Pieter Rein ten Wolde

Polymers with active segments constitute prospective future materials and are used as a model for some biological systems such as chromatin. The directions of the active forces are typically introduced with temporal or spatial correlations…

软凝聚态物质 · 物理学 2024-11-25 Adam H. T. P. Höfler , Iurii Chubak , Christos N. Likos , Jan Smrek