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Cell nuclei segmentation is one of the most important tasks in the analysis of biomedical images. With ever-growing sizes and amounts of three-dimensional images to be processed, there is a need for better and faster segmentation methods.…

计算机视觉与模式识别 · 计算机科学 2017-02-20 Julian Arz , Peter Sanders , Johannes Stegmaier , Ralf Mikut

Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

图像与视频处理 · 电气工程与系统科学 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

Background Analyzing images to accurately estimate the number of different cell types in the brain using automatic methods is a major objective in neuroscience. The automatic and selective detection and segmentation of neurons would be an…

图像与视频处理 · 电气工程与系统科学 2024-10-07 Antonio LaTorre , Lidia Alonso-Nanclares , José María Peña , Javier De Felipe

Modeling the 3D structures of cells and tissues is crucial in biology. Sequential cross-sectional images from electron microscopy provide high-resolution intracellular structure information. The segmentation of complex cell structures…

定量方法 · 定量生物学 2025-02-14 Jin Kousaka , Atsuko H. Iwane , Yuichi Togashi

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

计算机视觉与模式识别 · 计算机科学 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

Fluorescence microscopy is an essential tool for the analysis of 3D subcellular structures in tissue. An important step in the characterization of tissue involves nuclei segmentation. In this paper, a two-stage method for segmentation of…

图像与视频处理 · 电气工程与系统科学 2020-01-10 David Joon Ho , Shuo Han , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

We propose a 3D convolutional neural network to simultaneously segment and detect cell nuclei in confocal microscopy images. Mirroring the co-dependency of these tasks, our proposed model consists of two serial components: the first part…

图像与视频处理 · 电气工程与系统科学 2018-09-07 Sundaresh Ram , Vicky T. Nguyen , Kirsten H. Limesand , Mert R. Sabuncu

Automated sample preparation and electron microscopy enables acquisition of very large image data sets. These technical advances are of special importance to the field of neuroanatomy, as 3D reconstructions of neuronal processes at the nm…

Cell boundary information is crucial for analyzing cell behaviors from time-lapse microscopy videos. Existing supervised cell segmentation tools, such as ImageJ, require tuning various parameters and rely on restrictive assumptions about…

应用统计 · 统计学 2026-01-27 Laura Baracaldo , Blythe King , Haoran Yan , Yizi Lin , Nina Miolane , Mengyang Gu

Segmentation of cell nuclei in microscopy images is a prevalent necessity in cell biology. Especially for three-dimensional datasets, manual segmentation is prohibitively time-consuming, motivating the need for automated methods.…

计算机视觉与模式识别 · 计算机科学 2020-02-10 Peter Hirsch , Dagmar Kainmueller

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

计算机视觉与模式识别 · 计算机科学 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

Cell segmentation in microscopy is a challenging problem, since cells are often asymmetric and densely packed. This becomes particularly challenging for extremely large images, since manual intervention and processing time can make…

图像与视频处理 · 电气工程与系统科学 2019-06-19 Mahsa Lotfollahi , Sebastian Berisha , Leila Saadatifard , Laura Montier , Jokubas Ziburkus , David Mayerich

Advances in fluorescence microscopy enable acquisition of 3D image volumes with better image quality and deeper penetration into tissue. Segmentation is a required step to characterize and analyze biological structures in the images and…

计算机视觉与模式识别 · 计算机科学 2019-04-16 Chichen Fu , Soonam Lee , David Joon Ho , Shuo Han , Paul Salama , Kenneth W. Dunn , Edward J. Delp

We present a novel method for cell segmentation in microscopy images which is inspired by the Generative Adversarial Neural Network (GAN) approach. Our framework is built on a pair of two competitive artificial neural networks, with a…

计算机视觉与模式识别 · 计算机科学 2018-09-14 Assaf Arbelle , Tammy Riklin Raviv

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

图像与视频处理 · 电气工程与系统科学 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

光学 · 物理学 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai

Automated cell segmentation in microscopy images is essential for biomedical research, yet conventional methods are labor-intensive and prone to error. While deep learning-based approaches have proven effective, they often require large…

计算机视觉与模式识别 · 计算机科学 2024-11-20 Rüveyda Yilmaz , Kaan Keven , Yuli Wu , Johannes Stegmaier

The quantitative analysis of 3D confocal microscopy images of the shoot apical meristem helps understanding the growth process of some plants. Cell segmentation in these images is crucial for computational plant analysis and many automated…

计算机视觉与模式识别 · 计算机科学 2017-10-30 Thiago V. Spina , Johannes Stegmaier , Alexandre X. Falcão , Elliot Meyerowitz , Alexandre Cunha

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

计算机视觉与模式识别 · 计算机科学 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

Annotation is a major hurdle in the semantic segmentation of microscopy images and volumes due to its prerequisite expertise and effort. This work enables the training of semantic segmentation networks on images with only a single point for…

计算机视觉与模式识别 · 计算机科学 2021-03-22 Shijie Li , Neel Dey , Katharina Bermond , Leon von der Emde , Christine A. Curcio , Thomas Ach , Guido Gerig
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