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Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

生物物理 · 物理学 2007-08-27 James H. Rice

Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of…

光学 · 物理学 2013-05-30 Osip Schwartz , Dan Oron

The optical diffraction limit, formulated by Abbe 140 years ago, imposes a bound on imaging resolution in classical optics. Over the last twenty years, many theoretical schemes have been presented for overcoming the diffraction barrier in…

量子物理 · 物理学 2013-07-12 O. Schwartz , J. M. Levitt , R. Tenne , S. Itzhakov , Z. Deutsch , D. Oron

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

生物物理 · 物理学 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

We numerically investigate the role of quantum fluctuations in superresolution of optical objects. First, we confirm that when quantum fluctuations are not taken into account, one can easily improve the resolution by one order of magnitude…

量子物理 · 物理学 2007-05-23 Vladislav N. Beskrovnyy , Mikhail I. Kolobov

We proposed a method to achieve superresolved optical imaging without beating the diffraction limit of light. This is achieved by magnifying the ideal optical image of the object through higher-order spatial frequency generation while…

光学 · 物理学 2016-08-08 Zhixiang Li , Jianji Liu , Guoquan Zhang

Motivated by the importance of optical microscopes to science and engineering, scientists have pondered for centuries how to improve their resolution and the existence of fundamental resolution limits. In recent years, a new class of…

量子物理 · 物理学 2015-07-28 Mankei Tsang

Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

First discovered by Ernest Abbe in 1873, the resolution limit of a far-field microscope is considered determined by the numerical aperture and wavelength of light, approximately $\lambda$/2NA. With the advent of modern fluorescence…

光学 · 物理学 2023-08-02 Yilun Li , Fang Huang

I explain what is, what is not, and what is only sort of superresolution microscopy. I discuss optical resolution, first in terms of diffraction theory, then in terms of linear systems theory, and finally in terms of techniques that use…

光学 · 物理学 2015-04-02 John Bechhoefer

No imaging apparatus can produce perfect images: spatial resolution is limited by the Rayleigh diffraction bound that is a consequence of the imager's finite spatial extent. We show some N-photon strategies that permit resolution of details…

量子物理 · 物理学 2009-02-25 Vittorio Giovannetti , Seth Lloyd , Lorenzo Maccone , Jeffrey H. Shapiro

We derive the fundamental limit to the resolution of far-field optical imaging, and demonstrate that, while a bound to the resolution of a fundamental nature does exit, contrary to the conventional wisdom it is neither exactly equal to nor…

光学 · 物理学 2019-03-14 Evgenii Narimanov

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Resolving sources beyond the diffraction limit is important in imaging, communications, and metrology. Current image-based methods of super-resolution require phase information (either of the source points or an added filter) and perfect…

光学 · 物理学 2025-12-16 S. A. Wadood , Shaurya Aarav , Kevin Liang , Jason W Fleischer

Visualization in the virtual image formed by dielectric microparticles has been shown to enable the distinction of objects that remain indistinguishable under direct observation. We perform the resolution analysis based on a full…

Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record…

光学 · 物理学 2021-08-26 Zengbo Wang

Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

生物物理 · 物理学 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

图像与视频处理 · 电气工程与系统科学 2019-12-10 Edward Y. Sheffield

The resolution of optical imaging devices is ultimately limited by the diffraction of light. To circumvent this limit, modern super-resolution microscopy techniques employ active interaction with the object by exploiting its optical…

光学 · 物理学 2022-01-05 A. A. Pushkina , G. Maltese , J. I. Costa-Filho , P. Patel , A. I. Lvovsky
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