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Gene delivery of nucleic acid to the cell nucleus is a fundamental step in gene therapy. In this review of modeling drug and gene delivery, we focus on the particular stage of plasmid DNA or virus cytoplasmic trafficking. A challenging…

亚细胞过程 · 定量生物学 2011-09-08 Carlo Amoruso , Thibault Lagache , David Holcman

Virus trafficking is fundamental for infection success and plasmid cytosolic trafficking is a key step of gene delivery. Based on the main physical properties of the cellular transport machinery such as microtubules, motor proteins, our…

定量方法 · 定量生物学 2007-12-21 Thibault Lagache , David Holcman

Active cellular transport is a fundamental mechanism for protein and vesicle delivery, cell cycle and molecular degradation. Viruses can hijack the transport system and use it to reach the nucleus. Most transport processes consist of…

定量方法 · 定量生物学 2009-11-13 T. Lagache , D. Holcman

Nucleocytoplasmic transport is essential for cellular function, presenting a canonical example of rapid molecular sorting inside cells. It consists of a coordinated interplay between import/export of molecules in/out the cell nucleus. Here,…

生物物理 · 物理学 2026-04-24 S. Alex Rautu , Alexandra Zidovska , Michael J. Shelley

The transport of macromolecules, such as DNA, through the cytoskeleton is critical to wide-ranging cellular processes from cytoplasmic streaming to transcription. The rigidity and steric hindrances imparted by the network of filaments…

The more we learn about the cytoplasm of cells, the more we realise that the cytoplasm is not uniform but instead is highly inhomogeneous. In any inhomogeneous solution, there are concentration gradients, and particles move either up or…

亚细胞过程 · 定量生物学 2019-04-03 Richard P. Sear

The nuclear pore supports molecular communication between cytoplasm and nucleus in eukaryotic cells. Selective transport of proteins is mediated by soluble receptors, whose regulation by the small GTPase Ran leads to cargo accumulation in,…

亚细胞过程 · 定量生物学 2009-03-17 R. B. Kopito , M. Elbaum

Single-cell trajectory analysis aims to reconstruct the biological developmental processes of cells as they evolve over time, leveraging temporal correlations in gene expression. During cellular development, gene expression patterns…

应用统计 · 统计学 2026-03-30 Junhao Zhu , Kevin Zhang , Zhaolei Zhang , Dehan Kong

Nuclear pore complexes are constantly confronted by large fluxes of macromolecules and macromolecular complexes that need to get into and out of the nucleus. Such bi-directional traffic occurring in a narrow channel can easily lead to…

生物物理 · 物理学 2008-07-22 Ruti Kapon , Alon Topchik , David Mukamel , and Ziv Reich

Many cellular networks rely on the regulated transport of their components to transduce extracellular information into precise intracellular signals. The dynamics of these networks is typically described in terms of compartmentalized…

亚细胞过程 · 定量生物学 2011-11-18 Jose M. G. Vilar , Leonor Saiz

Intracellular transport is essential for maintaining proper cellular function in most eukaryotic cells, with perturbations in active transport resulting in several types of disease. Efficient delivery of critical cargos to specific…

生物物理 · 物理学 2016-08-16 David Ando , Nickolay Korabel , Kerwyn Casey Huang , Ajay Gopinathan

The ejection of DNA from a bacterial virus (``phage'') into its host cell is a biologically important example of the translocation of a macromolecular chain along its length through a membrane. The simplest mechanism for this motion is…

生物大分子 · 定量生物学 2009-11-11 Mandar M. Inamdar , William M. Gelbart , Rob Phillips

It has been observed that the growth of the nucleus and the cytoplasm is coordinated during cell growth, resulting in a nearly constant nuclear-to-cell volume ratio (N/C) throughout the cell cycle. Previous studies have shown that the N/C…

细胞行为 · 定量生物学 2025-10-29 Xuesong Bai , Thomas G. Fai

Living cells display a remarkable capacity to compartmentalize their functional biochemistry. A particularly fascinating example is the cell nucleus. Exchange of macromolecules between the nucleus and the surrounding cytoplasm does not…

亚细胞过程 · 定量生物学 2017-04-19 Ching-Hao Wang , Pankaj Mehta , Michael Elbaum

It is increasingly common to encounter data from dynamic processes captured by static cross-sectional measurements over time, particularly in biomedical settings. Recent attempts to model individual trajectories from this data use optimal…

机器学习 · 统计学 2020-07-28 Alexander Tong , Jessie Huang , Guy Wolf , David van Dijk , Smita Krishnaswamy

The transport of particles in cells is influenced by the properties of intracellular networks they traverse while searching for localized target regions or reaction partners. Moreover, given the rapid turnover in many intracellular…

Internet, social media, neuronal or blood vessel are organized in complex networks. These networks are characterized by several quantities such as the underlying graph connectivity (topology), how they grow in time, scaling laws or by the…

亚细胞过程 · 定量生物学 2018-10-18 Matteo Dora , David Holcman

The spatial localization or sequestering of motile cargo and their dispersal within cells is an important process in a number of physiological contexts. The morphology of the cytoskeletal network, along which active, motor-driven…

生物物理 · 物理学 2019-07-16 Bryan Maelfeyt , Ajay Gopinathan

The ability of eukaryotic cells to squeeze through constrictions is limited by the stiffness of their large and rigid nucleus. However, migrating cells are often able to overcome this limitation and pass through constrictions much smaller…

生物物理 · 物理学 2023-12-20 Sirine Amiri , Yirui Zhang , Andonis Gerardos , Cécile Sykes , Pierre Ronceray

The infection pathway of virus in cytoplasm of a living cell is studied from the viewpoint of diffusion theory. The cytoplasm plays a role of a medium for stochastic motion of the virus contained in the endosome as well as the free virus.…

生物物理 · 物理学 2010-03-04 Yuichi Itto
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