Information limits of optical microscopy: application to fluorescently labelled tissue section
Medical Physics
2016-12-15 v1 Optics
Abstract
The article demonstrates some less known principles of image build-up in diffractive microscopy and their usage in analysis unravelling the smallest localized information about the original object - an electromagnetic centroid. In fluorescence, the electromagnetic centroid is naturally at the position of the fluorophore. The usage of an information-entropic variable - a point divergence gain - is demonstrated for finding the most localized position of the object's representation, generally of the size of a voxel (3D pixel). These spatial pixels can be qualitatively classified and used for reconstruction of the 3D structures with precision comparable with electron microscopy.
Cite
@article{arxiv.1612.04368,
title = {Information limits of optical microscopy: application to fluorescently labelled tissue section},
author = {Renata Rychtarikova and Georg Steiner and Michael B. Fischer and Dalibor Stys},
journal= {arXiv preprint arXiv:1612.04368},
year = {2016}
}
Comments
12 pages, 6 figures