Direct 3D imaging through spatial coherence of light
Abstract
Wide-field imaging is widely adopted due to its fast acquisition, cost-effectiveness and ease of use. Its extension to direct volumetric applications, however, is burdened by the trade-off between resolution and depth of field (DOF), dictated by the numerical aperture of the system. We demonstrate that such trade-off is not intrinsic to wide-field imaging, but stems from the spatial incoherence of light: images obtained through spatially coherent illumination are shown to have resolution and DOF independent of the numerical aperture. This fundamental discovery enabled us to demonstrate an optimal combination of coherent resolution-DOF enhancement and incoherent tomographic sectioning for scanning-free, wide-field 3D microscopy on a multicolor histological section.
Cite
@article{arxiv.2311.16002,
title = {Direct 3D imaging through spatial coherence of light},
author = {Gianlorenzo Massaro and Barbara Barile and Giuliano Scarcelli and Francesco V. Pepe and Grazia Paola Nicchia and Milena D'Angelo},
journal= {arXiv preprint arXiv:2311.16002},
year = {2023}
}
Comments
17 pages, 6 figures. Supplemental document available upon request to the authors. Submitted to Lasers and Photonics Reviews