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Parallel-beam X-ray computed tomography (CT) and electrical impedance tomography (EIT) are two imaging modalities which stem from completely different underlying physics, and for decades have been thought to have little in common either…

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

The diagnosis of cancer is mainly performed by visual analysis of the pathologists, through examining the morphology of the tissue slices and the spatial arrangement of the cells. If the microscopic image of a specimen is not stained, it…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Pegah Salehi , Abdolah Chalechale

In this paper, we develop a complete pipeline for stain normalization, segmentation, and classification of nuclei in hematoxylin and eosin (H&E) stained breast cancer histopathology images. In the first step, we use a CNN-based stain…

Computer Vision and Pattern Recognition · Computer Science 2018-11-12 Edwin Yuan , Junkyo Suh

Immunohistochemistry (IHC) has transformed clinical pathology by enabling the visualization of specific proteins within tissue sections. However, traditional IHC requires one tissue section per stain, exhibits section-to-section…

Structured Illumination Microscopy (SIM) enables rapid, high-contrast optical sectioning of fresh tissue without staining or physical sectioning, making it promising for intraoperative and point-of-care diagnostics. Recent foundation and…

Accurate histopathological diagnosis often requires multiple differently stained tissue sections, a process that is time-consuming, labor-intensive, and environmentally taxing due to the use of multiple chemical stains. Recently, virtual…

Computer Vision and Pattern Recognition · Computer Science 2025-09-18 Jiabo MA , Wenqiang Li , Jinbang Li , Ziyi Liu , Linshan Wu , Fengtao Zhou , Li Liang , Ronald Cheong Kin Chan , Terence T. W. Wong , Hao Chen

Accurate and scalable cancer diagnosis remains a critical challenge in modern pathology, particularly for malignancies such as breast, prostate, bone, and cervical, which exhibit complex histological variability. In this study, we propose a…

Image and Video Processing · Electrical Eng. & Systems 2025-08-18 Faisal Ahmed

Accurate semantic segmentation for histopathology image is crucial for quantitative tissue analysis and downstream clinical modeling. Recent segmentation foundation models have improved generalization through large-scale pretraining, yet…

Computer Vision and Pattern Recognition · Computer Science 2026-01-26 Peixian Liang , Songhao Li , Shunsuke Koga , Yutong Li , Zahra Alipour , Yucheng Tang , Daguang Xu , Zhi Huang

By 2025, holotomography (HT) has matured from a niche optical modality into a versatile platform for quantitative, label-free imaging in biomedicine. By reconstructing the three-dimensional refractive-index (RI) distribution of cells and…

Biological Physics · Physics 2026-01-07 YongKeun Park

This paper introduces a Virtual Immunohistochemistry Multiplex staining (VIMs) model designed to generate multiple immunohistochemistry (IHC) stains from a single hematoxylin and eosin (H&E) stained tissue section. IHC stains are crucial in…

Image and Video Processing · Electrical Eng. & Systems 2024-07-30 Shikha Dubey , Yosep Chong , Beatrice Knudsen , Shireen Y. Elhabian

Purpose: In digital histopathology, virtual multi-staining is important for diagnosis and biomarker research. Additionally, it provides accurate ground-truth for various deep-learning tasks. Virtual multi-staining can be obtained using…

Image and Video Processing · Electrical Eng. & Systems 2023-12-15 Johannes Lotz , Nick Weiss , Jeroen van der Laak , Stefan Heldmann

With the advent of digital scanners and deep learning, diagnostic operations may move from a microscope to a desktop. Hematoxylin and Eosin (H&E) staining is one of the most frequently used stains for disease analysis, diagnosis, and…

Computer Vision and Pattern Recognition · Computer Science 2023-08-28 Shikha Dubey , Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

The still unmet ability of following the fate of specific cells or molecules within the whole body would give an outstanding breakthrough in the comprehension of disease mechanisms and in the monitoring of therapeutic approaches. Our idea…

Hematoxylin and Eosin (H&E) has been the gold standard in tissue analysis for decades, however, tissue specimens stained in different laboratories vary, often significantly, in appearance. This variation poses a challenge for both…

Image and Video Processing · Electrical Eng. & Systems 2025-06-25 Umair Khan , Jouni Härkönen , Marjukka Friman , Leena Latonen , Teijo Kuopio , Pekka Ruusuvuori

Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

Computer Vision and Pattern Recognition · Computer Science 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan

Foundation models for interactive segmentation in 2D natural images and videos have sparked significant interest in building 3D foundation models for medical imaging. However, the domain gaps and clinical use cases for 3D medical imaging…

Computer Vision and Pattern Recognition · Computer Science 2024-11-26 Yufan He , Pengfei Guo , Yucheng Tang , Andriy Myronenko , Vishwesh Nath , Ziyue Xu , Dong Yang , Can Zhao , Benjamin Simon , Mason Belue , Stephanie Harmon , Baris Turkbey , Daguang Xu , Wenqi Li

The analysis of FFPE tissue sections stained with haematoxylin and eosin (H&E) or immunohistochemistry (IHC) is an essential part of the pathologic assessment of surgically resected breast cancer specimens. IHC staining has been broadly…

Due to its deficiency in prior knowledge (inductive bias), Vision Transformer (ViT) requires pre-training on large-scale datasets to perform well. Moreover, the growing layers and parameters in ViT models impede their applicability to…

Computer Vision and Pattern Recognition · Computer Science 2024-07-17 Chenhao Xu , Chang-Tsun Li , Chee Peng Lim , Douglas Creighton

The emergence of virtual staining technology provides a rapid and efficient alternative for researchers in tissue pathology. It enables the utilization of unlabeled microscopic samples to generate virtual replicas of chemically stained…

Image and Video Processing · Electrical Eng. & Systems 2025-01-08 Junjia Wang , Bo Xiong , You Zhou , Xun Cao , Zhan Ma