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Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…

Optics · Physics 2024-10-16 Doron Shterman , Guy Bartal

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Histopathology, particularly hematoxylin and eosin (H\&E) staining, plays a critical role in diagnosing and characterizing pathological conditions by highlighting tissue morphology. However, H\&E-stained images inherently lack molecular…

Computational Engineering, Finance, and Science · Computer Science 2025-01-28 Qing Wang , Wen-jie Chen , Bo Li , Jing Su , Guangyu Wang , Qianqian Song

Over the past decade, structured illumination microscopy (SIM) has found its niche in super-resolution (SR) microscopy due to its fast imaging speed and low excitation intensity. However, due to the significantly higher light dose compared…

Medical image segmentation remains a formidable challenge due to the label scarcity. Pre-training Vision Transformer (ViT) through masked image modeling (MIM) on large-scale unlabeled medical datasets presents a promising solution,…

Computer Vision and Pattern Recognition · Computer Science 2025-02-13 Fenghe Tang , Qingsong Yao , Wenxin Ma , Chenxu Wu , Zihang Jiang , S. Kevin Zhou

The landscape of skeleton-based action representation learning has evolved from Contrastive Learning (CL) to Masked Auto-Encoder (MAE) architectures. However, each paradigm faces inherent limitations: CL often overlooks fine-grained local…

Computer Vision and Pattern Recognition · Computer Science 2026-03-13 Jeonghyeok Do , Yun Chen , Geunhyuk Youk , Munchurl Kim

Fine-grained visual categorization (FGVC) aims at recognizing objects from similar subordinate categories, which is challenging and practical for human's accurate automatic recognition needs. Most FGVC approaches focus on the attention…

Computer Vision and Pattern Recognition · Computer Science 2022-09-01 Hongbo Sun , Xiangteng He , Yuxin Peng

Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple…

Optics · Physics 2020-07-15 Meiqi Li , Yaning Li , Wenhui Liu , Karl Zhanghao , Peng Xi

Masked image modeling (MIM) with transformer backbones has recently been exploited as a powerful self-supervised pre-training technique. The existing MIM methods adopt the strategy to mask random patches of the image and reconstruct the…

Computer Vision and Pattern Recognition · Computer Science 2024-02-05 Zhaohu Xing , Lei Zhu , Lequan Yu , Zhiheng Xing , Liang Wan

Structured illumination microscopy (SIM) has emerged as a widely adopted super-resolution fluorescence imaging modality, offering high speed, low phototoxicity, large field-of-view, and compatibility with conventional probes. However, when…

Image and Video Processing · Electrical Eng. & Systems 2026-03-19 Jiaming Qian , Jing Feng , Hongjun Wu , Maoxian Zhang , Dongqin Lu , Tianchi Kang , Xinyu Han , Qian Chen , Chao Zuo

Current pathology workflow involves staining of thin tissue slices, which otherwise would be transparent, followed by manual investigation under the microscope by a trained pathologist. While the hematoxylin and eosin (H&E) stain is…

Medical Physics · Physics 2021-05-04 Jingfang "Kelly" Zhang , Yuchen R. He , Nahil Sobh , Gabriel Popescu

Hyperspectral imaging (HSI) enables detailed land cover classification, yet low spatial resolution and sparse annotations pose significant challenges. We present a label-efficient framework that leverages spatial features from a frozen…

Computer Vision and Pattern Recognition · Computer Science 2025-12-04 Yuzhen Hu , Biplab Banerjee , Saurabh Prasad

Semantic segmentation of electron microscopy (EM) images of biological samples remains a challenge in the life sciences. EM data captures details of biological structures, sometimes with such complexity that even human observers can find it…

Computer Vision and Pattern Recognition · Computer Science 2025-11-03 Sheida Rahnamai Kordasiabi , Damian Dalle Nogare , Florian Jug

Structured illumination microscopy (SIM) reconstructs a super-resolved image from multiple raw images captured with different illumination patterns; hence, acquisition speed is limited, making it unsuitable for dynamic scenes. We propose a…

Optics · Physics 2025-06-17 Ruiming Cao , Fanglin Linda Liu , Li-Hao Yeh , Laura Waller

Regularization is essential in deep learning to enhance generalization and mitigate overfitting. However, conventional techniques often rely on heuristics, making them less reliable or effective across diverse settings. We propose Self…

Computer Vision and Pattern Recognition · Computer Science 2025-09-24 Xiuding Cai , Yaoyao Zhu , Linjie Fu , Dong Miao , Yu Yao

Hematoxylin and Eosin (H&E) staining is a cornerstone of pathological analysis, offering reliable visualization of cellular morphology and tissue architecture for cancer diagnosis, subtyping, and grading. Immunohistochemistry (IHC) staining…

Image and Video Processing · Electrical Eng. & Systems 2025-06-23 Amit Das , Naofumi Tomita , Kyle J. Syme , Weijie Ma , Paige O'Connor , Kristin N. Corbett , Bing Ren , Xiaoying Liu , Saeed Hassanpour

Sub-diffraction resolution, gentle sample illumination, and the possibility to image in multiple colors make Structured Illumination Microscopy (SIM) an imaging technique which is particularly well suited for live cell observations. Here,…

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Foundation models in computational pathology promise to unlock the development of new clinical decision support systems and models for precision medicine. However, there is a mismatch between most clinical analysis, which is defined at the…

Learning meaningful and interpretable representations from high-dimensional volumetric magnetic resonance (MR) images is essential for advancing personalized medicine. While Vision Transformers (ViTs) have shown promise in handling image…

Computer Vision and Pattern Recognition · Computer Science 2024-09-13 Qingqiao Hu , Daoan Zhang , Jiebo Luo , Zhenyu Gong , Benedikt Wiestler , Jianguo Zhang , Hongwei Bran Li