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Expression of human epidermal growth factor receptor 2 (HER2) is an important biomarker in breast cancer patients who can benefit from cost-effective automatic Hematoxylin and Eosin (H\&E) HER2 scoring. However, developing such scoring…

Computer Vision and Pattern Recognition · Computer Science 2024-11-11 Rawan S. Abdulsadig , Bryan M. Williams , Nikolay Burlutskiy

Histological staining is a vital step used to diagnose various diseases and has been used for more than a century to provide contrast to tissue sections, rendering the tissue constituents visible for microscopic analysis by medical experts.…

Image and Video Processing · Electrical Eng. & Systems 2020-05-08 Yijie Zhang , Kevin de Haan , Yair Rivenson , Jingxi Li , Apostolos Delis , Aydogan Ozcan

Immunohistochemical (IHC) staining provides crucial molecular characterization of tissue samples and plays an indispensable role in the clinical examination and diagnosis of cancers. However, compared with the commonly used Hematoxylin and…

Computer Vision and Pattern Recognition · Computer Science 2026-01-07 Mingzhou Jiang , Jiaying Zhou , Nan Zeng , Mickael Li , Qijie Tang , Chao He , Huazhu Fu , Honghui He

Early and accurate diagnosis of parkinsonian syndromes is critical to provide appropriate care to patients and for inclusion in therapeutic trials. The red nucleus is a structure of the midbrain that plays an important role in these…

Multi-focus image fusion (MFIF) aims to yield an all-focused image from multiple partially focused inputs, which is crucial in applications cover sur-veillance, microscopy, and computational photography. However, existing methods struggle…

Computer Vision and Pattern Recognition · Computer Science 2025-09-29 Haoyu Li , XiaoSong Li

Multi-modality image fusion (MMIF) combines complementary information from different image modalities to provide a comprehensive and objective interpretation of scenes. However, existing fusion methods cannot resist different weather…

Computer Vision and Pattern Recognition · Computer Science 2026-03-17 Xilai Li , Wuyang Liu , Xiaosong Li , Fuqiang Zhou , Huafeng Li , Feiping Nie

In digital pathology, cell detection and classification are often prerequisites to quantify cell abundance and explore tissue spatial heterogeneity. However, these tasks are particularly challenging for multiplex immunohistochemistry (mIHC)…

Image and Video Processing · Electrical Eng. & Systems 2019-08-05 Yeman Brhane Hagos , Priya Lakshmi Narayanan , Ayse U. Akarca , Teresa Marafioti , Yinyin Yuan

Liquid biopsies (eg., blood draws) offer a less invasive and non-localized alternative to tissue biopsies for monitoring the progression of metastatic breast cancer (mBCa). Immunofluoresence (IF) microscopy is a tool to image and analyze…

Computer Vision and Pattern Recognition · Computer Science 2024-10-07 Evan Schwab , Bharat Annaldas , Nisha Ramesh , Anna Lundberg , Vishal Shelke , Xinran Xu , Cole Gilbertson , Jiyun Byun , Ernest T. Lam

Simultaneous identification and metabolic analysis of microbes with single-cell resolution and high throughput is necessary to answer the question of "who eats what, when, and where" in complex microbial communities. Here, we present a…

Biological Physics · Physics 2022-09-13 Yeran Bai , Zhongyue Guo , Fátima C. Pereira , Michael Wagner , Ji-Xin Cheng

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images…

Image and Video Processing · Electrical Eng. & Systems 2019-02-08 Yair Rivenson , Tairan Liu , Zhensong Wei , Yibo Zhang , Aydogan Ozcan

The segmentation of histopathological whole slide images into tumourous and non-tumourous types of tissue is a challenging task that requires the consideration of both local and global spatial contexts to classify tumourous regions…

Breast cancer is a health problem that affects mainly the female population. An early detection increases the chances of effective treatment, improving the prognosis of the disease. In this regard, computational tools have been proposed to…

Computer Vision and Pattern Recognition · Computer Science 2022-03-01 Steve Tsham Mpinda Ataky , Alessandro Lameiras Koerich

Multi-focus image fusion (MFIF) addresses the depth-of-field (DOF) limitations of optical lenses, where only objects within a specific range appear sharp. Although traditional and deep learning methods have advanced the field, challenges…

Computer Vision and Pattern Recognition · Computer Science 2025-10-23 Luca Piano , Peng Huanwen , Radu Ciprian Bilcu

The overexpression of the human epidermal growth factor receptor 2 (HER2) in breast cells is a key driver of HER2-positive breast cancer, a highly aggressive subtype requiring precise diagnosis and targeted therapy. Immunohistochemistry…

Image and Video Processing · Electrical Eng. & Systems 2026-03-10 Sara Rehmat , Hafeez Ur Rehman , Byeong-Gwon Kang , Sarra Ayouni , Yunyoung Nam

With the growing application of transformer in computer vision, hybrid architecture that combine convolutional neural networks (CNNs) and transformers demonstrates competitive ability in medical image segmentation. However, direct fusion of…

Computer Vision and Pattern Recognition · Computer Science 2024-12-02 Xiwei Liu , Min Xu , Qirong Ho

Vision Transformers (ViTs) have gained significant popularity in the natural image domain but have been less successful in 3D medical image segmentation. Nevertheless, 3D ViTs are particularly interesting for large medical imaging volumes…

Computer Vision and Pattern Recognition · Computer Science 2025-10-30 Benjamin Jin , Grant Mair , Joanna M. Wardlaw , Maria del C. Valdés Hernández

Traditional staining normalization approaches, e.g. Macenko, typically rely on the choice of a single representative reference image, which may not adequately account for the diverse staining patterns of datasets collected in practical…

Image and Video Processing · Electrical Eng. & Systems 2024-06-11 Desislav Ivanov , Carlo Alberto Barbano , Marco Grangetto

In histopathology, tissue samples are often larger than a standard microscope slide, making stitching of multiple fragments necessary to process entire structures such as tumors. Automated stitching is a prerequisite for scaling analysis,…

Computer Vision and Pattern Recognition · Computer Science 2025-08-06 Stefan Brandstätter , Maximilian Köller , Philipp Seeböck , Alissa Blessing , Felicitas Oberndorfer , Svitlana Pochepnia , Helmut Prosch , Georg Langs

Processing giga-pixel whole slide histopathology images (WSI) is a computationally expensive task. Multiple instance learning (MIL) has become the conventional approach to process WSIs, in which these images are split into smaller patches…

Computer Vision and Pattern Recognition · Computer Science 2023-07-06 Ramin Nakhli , Puria Azadi Moghadam , Haoyang Mi , Hossein Farahani , Alexander Baras , Blake Gilks , Ali Bashashati

The semantic segmentation of skin lesions is an important and common initial task in the computer aided diagnosis of dermoscopic images. Although deep learning-based approaches have considerably improved the segmentation accuracy, there is…

Computer Vision and Pattern Recognition · Computer Science 2020-03-24 Kumar Abhishek , Ghassan Hamarneh , Mark S. Drew