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A device based on a three-block Fresnel zone plate interferometer is proposed for hard X-ray phase-contrast imaging. The device combines a low requirement for the coherence of the initial radiation (the interferometer operates in the…

Instrumentation and Detectors · Physics 2021-04-22 L. A. Haroutunyan

We propose an imaging system and methodology for mapping soft-tissue samples in three dimensions, with micron-scale and isotropic spatial resolution, with low-concentrations as well as in the absence of heavy metal staining. We used hard…

Instrumentation and Detectors · Physics 2023-12-21 Michela Esposito , Nicole Schieber , Alessandro Olivo , Yannick Schwab , Marco Endrizzi

Since the invention of Zernike phase contrast method in 1930, it has been widely used in optical microscopy and more recently in X-ray microscopy. Considering the image contrast is a mixture of absorption and phase information, we recently…

Optics · Physics 2015-12-03 Heng Chen , Kun Gao , Dajiang Wang , Li Song , Zhili Wang

A new method for identifying crystalline phases in X-ray diffraction data has been proposed, which is especially useful for the study of multiphase materials (more than eight - ten phases) with a relatively low content (less than 1 - 3…

Materials Science · Physics 2021-08-17 A. D. Skorbun , S. V. Gabielkov , I. V. Zhyganiuk

The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

Knowledge gained through X-ray crystallography fostered structural determination of materials and greatly facilitated the development of modern science and technology in the past century. Atomic details of sample structures is achievable by…

Optics · Physics 2016-09-14 Hong Yu , Ronghua Lu , Shensheng Han , Honglan Xie , Guohao Du , Tiqiao Xiao , Daming Zhu

We propose a Zernike phase contrast electron microscope that uses an intense laser focus to convert a phase image into a visible image. We present the relativistic quantum theory of the phase shift caused by the laser-electron-interaction,…

Instrumentation and Detectors · Physics 2010-09-10 Holger Mueller , Jian Jin , Radostin Danev , John Spence , Howard Padmore , Robert M. Glaeser

New lensless diffractive X-ray technic for micro-scale imaging of biological tissue is based on quantitative phase retrieval schemes. By incorporating refraction, this method yields improved contrast compared to purely absorption-based…

Numerical Analysis · Mathematics 2018-03-28 Victor Palamodov

Phase-plate transmission electron microscopy has recently regressed since a report that Volta phase-plate phase-contrast is less sensitive than non-phase-plate phase contrast, which leads to conventional defocusing phase-contrast. What…

Optics · Physics 2023-07-05 Kuniaki Nagayama

X-ray phase contrast imaging offers a way to visualize the internal structures of an object without the need to deposit any radiation, and thereby alleviate the main concern in x-ray diagnostic imaging procedures today. Grating-based…

We present a single-image numerical phase retrieval method for Zernike phase-contrast microscopy (ZPM) that addresses halo and shade-off artifacts, as well as the weak phase condition, without requiring hardware modifications. By employing…

In order to efficiently image a non-absorbing sample (a phase object), dedicated phase contrast optics are required. Typically, these optics are designed with the assumption that the sample is weakly scattering, implying a linear relation…

Optics · Physics 2020-10-22 Stewart Koppell , Mark Kasevich

Previous Zernike phase-plates, for transmission-electron-microscopy (TEM) have always been made out of carbon. A critical constraint on those phase-plates is their fast degradation during use. Here, a Zernike phase-plate consisting out of…

Materials Science · Physics 2017-08-18 Leonard F. Henrichs

We presents the results of study of focusing and imaging properties of double-lens system for hard x-ray radiation consisting of two Fresnel zone plates (ZP) made from silicon. We demonstrate for the first time the phenomenon of focusing by…

We explore the possibility of applying physical phase plates (PPs) in combination with aberration-corrected transmission electron microscopy. Phase-contrast transfer characteristics are calculated and compared for a thin-film based Zernike…

Instrumentation and Detectors · Physics 2022-05-10 Simon Hettler , Raul Arenal

We introduce a phase imaging mechanism for scanning transmission electron microscopy that exploits the complementary intensity changes of transmitted disks at different scattering angles. For scanning transmission electron microscopy, this…

Instrumentation and Detectors · Physics 2023-07-06 Binbin Wang , David W. McComb

The objective of this article is to study the behavior of electromagnetic field under X-ray diffraction by time-dependent deformed crystals. Derived system of differential equations looks like the Takagi equations in the case of…

Computational Physics · Physics 2007-05-23 Svetlana Sytova

Analysis of the imaging of some simple distributions of object phase by a phase plate of Zernike type shows that sharp transitions in the object phase are well transmitted. The low-frequency components of the complete object function are…

Instrumentation and Detectors · Physics 2013-07-26 C. J. Edgcombe

We outline some basics of imaging using both fully-coherent and partially-coherent X-ray beams, with an emphasis on phase-contrast imaging. We open with some of the basic notions of X-ray imaging, including the vacuum wave equations and the…

Image and Video Processing · Electrical Eng. & Systems 2021-11-16 David M. Paganin , Daniele Pelliccia

X-ray micro tomography is a non-destructive, three-dimensional inspection technique applied across a vast range of fields and disciplines, ranging from research to industrial, encompassing engineering, biology and medical research.…

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