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Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution…
We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. A 22-fold…
MINFLUX is an emerging super-resolution technology that measures the position of single fluorophores with nanometer precision using fewer photons than any other fluorescence imaging or tracking technique. Here, we derive simple and…
Localization of single fluorescent molecules is key for physicochemical and biophysical measurements such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy (SMLM). Recently a series of…
MINFLUX microscopy allows for localization of fluorophores with nanometer precision using targeted scanning with an illumination profile with a minimum. However, current scanning patterns and the overall procedure are based on heuristics,…
Localization microscopy enables imaging with resolutions that surpass the conventional optical diffraction limit. Notably, the MINFLUX method achieves super-resolution by shaping the excitation point-spread function (PSF) to minimize the…
In the past years, optical fluorescence microscopy (OFM) made steady progress towards increasing the localisation precision of fluorescent emitters in biological samples. The high precision achieved by these techniques has prompted new…
Single-particle imaging experiments of biomolecules at x-ray free-electron lasers (XFELs) require processing of hundreds of thousands (or more) of images that contain very few x-rays. Each low-flux image of the diffraction pattern is…
We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the…
Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…
Fluorescence microscopy is essential in biological and medical research, providing critical insights into cellular structures. However, limited by optical diffraction and background noise, a substantial amount of hidden information is still…
Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…
Single-image point cloud reconstruction must infer complete 3D geometry, including occluded parts, from a single RGB image. While diffusion-based reconstructors achieve high accuracy, they typically require many denoising iterations,…
Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…
Current Flash X-ray single-particle diffraction Imaging (FXI) experiments, which operate on modern X-ray Free Electron Lasers (XFELs), can record millions of interpretable diffraction patterns from individual biomolecules per day. Due to…
The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…
Transductive inference has been widely investigated in few-shot image classification, but completely overlooked in the recent, fast growing literature on adapting vision-langage models like CLIP. This paper addresses the transductive…
Single-molecule spectroscopy (SMS) is an exceptionally sensitive technique, but its inherently limited photon budget produces noisy data that can readily lead to subjective analyses, fitting errors, and reduced statistical power, obscuring…
To be able to resolve molecular-clusters it is crucial to access vital informations (such as, molecule density and cluster-size) that are key to understand disease progression and the underlying mechanism. Traditional single-molecule…
FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…