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Single-cell RNA sequencing (scRNA-seq) data analysis is crucial for biological research, as it enables the precise characterization of cellular heterogeneity. However, manual manipulation of various tools to achieve desired outcomes can be…

Artificial Intelligence · Computer Science 2024-07-16 Yihang Xiao , Jinyi Liu , Yan Zheng , Xiaohan Xie , Jianye Hao , Mingzhi Li , Ruitao Wang , Fei Ni , Yuxiao Li , Jintian Luo , Shaoqing Jiao , Jiajie Peng

Cell type annotation is critical for understanding cellular heterogeneity. Based on single-cell RNA-seq data and deep learning models, good progress has been made in annotating a fixed number of cell types within a specific tissue. However,…

Computation and Language · Computer Science 2025-04-08 Yuren Mao , Yu Mi , Peigen Liu , Mengfei Zhang , Hanqing Liu , Yunjun Gao

Single-cell RNA-seq data allow the quantification of cell type differences across a growing set of biological contexts. However, pinpointing a small subset of genomic features explaining this variability can be ill-defined and…

Machine Learning · Statistics 2022-07-29 Nabeel Sarwar , Wilson Gregory , George A Kevrekidis , Soledad Villar , Bianca Dumitrascu

Cell type annotation is a critical yet laborious step in single-cell RNA sequencing analysis. We present a trustworthy large language model (LLM)-agent, CellTypeAgent, which integrates LLMs with verification from relevant databases.…

Genomics · Quantitative Biology 2025-05-15 Jiawen Chen , Jianghao Zhang , Huaxiu Yao , Yun Li

Single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to analyze gene expression at the resolution of individual cells, providing unprecedented insights into cellular heterogeneity and complex biological systems. This paper…

Other Quantitative Biology · Quantitative Biology 2024-06-11 Megha Patel , Nimish Magre , Himanshi Motwani , Nik Bear Brown

Single-cell RNA sequencing (scRNA-seq) provides a high throughput, quantitative and unbiased framework for scientists in many research fields to identify and characterize cell types within heterogeneous cell populations from various…

Quantitative Methods · Quantitative Biology 2022-09-28 Yeganeh Madadi , Aboozar Monavarfeshani , Hao Chen , W. Daniel Stamer , Robert W. Williams , Siamak Yousefi

Single-cell RNA sequencing (scRNAseq) is rapidly advancing our understanding of cellular composition within complex tissues and organisms. A major limitation in most scRNAseq analysis pipelines is the reliance on manual annotations to…

Genomics · Quantitative Biology 2022-06-10 A. Ali Heydari , Oscar A. Davalos , Katrina K. Hoyer , Suzanne S. Sindi

Background: Single-cell RNA sequencing (scRNA-seq) yields valuable insights about gene expression and gives critical information about complex tissue cellular composition. In the analysis of single-cell RNA sequencing, the annotations of…

Genomics · Quantitative Biology 2023-03-29 Xiaowen Cao , Li Xing , Elham Majd , Hua He , Junhua Gu , Xuekui Zhang

Over the past decade, the revolution in single-cell sequencing has enabled the simultaneous molecular profiling of various modalities across thousands of individual cells, allowing scientists to investigate the diverse functions of complex…

Computation and Language · Computer Science 2024-12-05 Junhao Liu , Siwei Xu , Lei Zhang , Jing Zhang

Cell type annotation is a fundamental step in the analysis of single-cell RNA sequencing (scRNA-seq) data. In practice, human experts often rely on the structure revealed by principal component analysis (PCA) followed by $k$-nearest…

Genomics · Quantitative Biology 2026-02-10 Tianxiang Hu , Chenyi Zhou , Jiaxiang Liu , Jiongxin Wang , Ruizhe Chen , Haoxiang Xia , Gaoang Wang , Jian Wu , Zuozhu Liu

Cell type identification from single-cell transcriptomic data is a common goal of single-cell RNA sequencing (scRNAseq) data analysis. Neural networks have been employed to identify cell types from scRNAseq data with high performance.…

Genomics · Quantitative Biology 2020-05-11 Xishuang Dong , Shanta Chowdhury , Uboho Victor , Xiangfang Li , Lijun Qian

Reliability in cell type annotation is challenging in single-cell RNA-sequencing data analysis because both expert-driven and automated methods can be biased or constrained by their training data, especially for novel or rare cell types.…

Quantitative Methods · Quantitative Biology 2024-09-25 Wenjin Ye , Yuanchen Ma , Junkai Xiang , Hongjie Liang , Tao Wang , Qiuling Xiang , Andy Peng Xiang , Wu Song , Weiqiang Li , Weijun Huang

Single-cell RNA sequencing (scRNA-seq) technology enables systematic delineation of cellular states and interactions, providing crucial insights into cellular heterogeneity. Building on this potential, numerous computational methods have…

Genomics · Quantitative Biology 2025-11-11 Ping Xu , Zaitian Wang , Zhirui Wang , Pengjiang Li , Ran Zhang , Gaoyang Li , Hanyu Xie , Jiajia Wang , Yuanchun Zhou , Pengfei Wang

Single-cell RNA sequencing has transformed our ability to identify diverse cell types and their transcriptomic signatures. However, annotating these signatures-especially those involving poorly characterized genes-remains a major challenge.…

In recent years, several unsupervised cell segmentation methods have been presented, trying to omit the requirement of laborious pixel-level annotations for the training of a cell segmentation model. Most if not all of these methods handle…

Computer Vision and Pattern Recognition · Computer Science 2023-12-04 Mehdi Naouar , Gabriel Kalweit , Anusha Klett , Yannick Vogt , Paula Silvestrini , Diana Laura Infante Ramirez , Roland Mertelsmann , Joschka Boedecker , Maria Kalweit

In recent years, single cell RNA sequencing has become a widely used technique to study cellular diversity and function. However, accurately annotating cell types from single cell data has been a challenging task, as it requires extensive…

Genomics · Quantitative Biology 2023-04-07 Zehua Zeng , Hongwu Du

Single-cell RNA sequencing has transformed biology by enabling the measurement of gene expression at cellular resolution, providing information for cell types, states, and disease contexts. Recently, single-cell foundation models have…

Machine Learning · Computer Science 2025-10-13 Oussama Kharouiche , Aris Markogiannakis , Xiao Fei , Michail Chatzianastasis , Michalis Vazirgiannis

Recent breakthroughs in single-cell technology have ushered in unparalleled opportunities to decode the molecular intricacy of intricate biological systems, especially those linked to diseases unique to humans. However, these progressions…

Genomics · Quantitative Biology 2025-08-26 Huan Zhao , Yiming Liu , Jina Yao , Ling Xiong , Zexin Zhou , Zixing Zhang

As single-cell RNA sequencing datasets grow in adoption, scale, and complexity, data analysis remains a bottleneck for many research groups. Although frontier AI agents have improved dramatically at software engineering and general data…

Genomics · Quantitative Biology 2026-02-11 Kenny Workman , Zhen Yang , Harihara Muralidharan , Aidan Abdulali , Hannah Le

Cell microscopy data are abundant; however, corresponding segmentation annotations remain scarce. Moreover, variations in cell types, imaging devices, and staining techniques introduce significant domain gaps between datasets. As a result,…

Machine Learning · Computer Science 2026-01-27 Rüveyda Yilmaz , Zhu Chen , Yuli Wu , Johannes Stegmaier
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