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Different studies in x-ray microscopy have arrived at conflicting conclusions about the dose efficiency of imaging modes involving the recording of intensity distributions in the near (Fresnel regime) or far (Fraunhofer regime) field…

Image and Video Processing · Electrical Eng. & Systems 2020-12-23 Ming Du , Doga Gursoy , Chris Jacobsen

FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…

Coherent interferometric scattering microscopy (iscat) enables nanoparticle tracking on microsecond timescales and with nanometer precision, and has become a key tool in structural and cellular biophysics. The achievable localization…

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

Mid-infrared photothermal microscopy is a new chemical imaging technology in which a visible beam senses the photothermal effect induced by a pulsed infrared laser. This technology provides infrared spectroscopic information at sub-micron…

Optics · Physics 2021-04-08 Yi Zhang , Haonan Zong , Cheng Zong , Yuying Tan , Meng Zhang , Yuewei Zhan , Ji-Xin Cheng

Optical-resolution fluorescence imaging through and within complex samples presents a significant challenge due to random light scattering, with substantial implications across multiple fields. While significant advancements in coherent…

Optics · Physics 2023-12-27 Gil Weinberg , Elad Sunray , Ori Katz

Tracking single molecules is instrumental for quantifying the transport of molecules and nanoparticles in biological samples, e.g., in brain drug delivery studies. Existing intensity-based localisation methods are not developed for imaging…

Despite advances in low-light level detection, single-photon methods such as photon correlation have rarely been used in the context of imaging. The few demonstrations, for example of sub-diffraction limited imaging utilizing quantum…

Optics · Physics 2017-03-14 Yonatan Israel , Ron Tenne , Dan Oron , Yaron Silberberg

Ptychography has become prominent at synchrotron facilities worldwide for characterizing biological and material specimens' topological structures and properties at the nanometer or atomic scale, due to its lens - less, highly quantitative…

Image and Video Processing · Electrical Eng. & Systems 2025-01-28 Tao Liu , Bingyang Wang , JiangTao Zhao , Maik Kahnt , Fucai Zhang

In fluorescence diffuse optical tomography (fDOT), the reconstruction of the fluorophore concentration inside the target body is usually carried out using a normalized Born approximation model where the measured fluorescent emission data is…

Computational Physics · Physics 2015-01-05 Meghdoot Mozumder , Tanja Tarvainen , Simon Arridge , Jari P. Kaipio , Cosimo d'Andrea , Ville Kolehmainen

We propose an unfolded accelerated projected-gradient descent procedure to estimate model and algorithmic parameters for image super-resolution and molecule localization problems in image microscopy. The variational lower-level constraint…

Numerical Analysis · Mathematics 2024-03-27 Silvia Bonettini , Luca Calatroni , Danilo Pezzi , Marco Prato

Here we report nonlinear focal modulation microscopy (NFOMM) to achieve super-resolution imaging. Abandoning the previous persistence on minimizing the size of Gaussian emission pattern by directly narrowing (e.g. Minimizing the detection…

Ultrasound localization microscopy offers new radiation-free diagnostic tools for vascular imaging deep within the tissue. Sequential localization of echoes returned from inert microbubbles with low-concentration within the bloodstream…

Medical Physics · Physics 2018-04-11 Oren Solomon , Ruud J. G. van Sloun , Hessel Wijkstra , Massimo Mischi , Yonina C. Eldar

Aligning beamlines at synchrotron light sources is a high-dimensional, expensive-to-sample optimization problem, as beams are focused using a series of dynamic optical components. Bayesian Optimization is an efficient machine learning…

Accelerator Physics · Physics 2024-08-14 Megha R. Narayanan , Thomas W. Morris

X-ray microtomography at synchrotron sources is fundamentally limited by the high radiation dose applied to the samples, which restricts investigations to non-native tissue states and thereby compromises the biological relevance of the…

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…

In developing medical interventions using untethered milli- and microrobots, ensuring safety and effectiveness relies on robust methods for detection, real-time tracking, and precise localization within the body. However, the inherent…

Robotics · Computer Science 2024-09-16 Husnu Halid Alabay , Tuan-Anh Le , Hakan Ceylan

Local Fourier analysis is a useful tool for predicting and analyzing the performance of many efficient algorithms for the solution of discretized PDEs, such as multigrid and domain decomposition methods. The crucial aspect of local Fourier…

Optimization and Control · Mathematics 2020-07-29 Jed Brown , Yunhui He , Scott MacLachlan , Matt Menickelly , Stefan M. Wild
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