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Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective Plane…

In recent years, interesting materials have emerged which are only available as micron-scale flakes, and whose novel physics might be better understood through broadband microwave spectroscopy; examples include twisted bilayer graphene [1],…

Mesoscale and Nanoscale Physics · Physics 2022-06-08 Antonio Levy , Neil Zimmerman

Biomedical challenges have become the de facto standard for benchmarking biomedical image analysis algorithms. While the number of challenges is steadily increasing, surprisingly little effort has been invested in ensuring high quality…

Computer Vision and Pattern Recognition · Computer Science 2019-12-06 Manuel Wiesenfarth , Annika Reinke , Bennett A. Landman , Manuel Jorge Cardoso , Lena Maier-Hein , Annette Kopp-Schneider

Materials science is undergoing profound changes due to advances in characterization instrumentation that have resulted in an explosion of data in terms of volume, velocity, variety and complexity. Harnessing these data for scientific…

The aim of this paper is to give an introductory review of the cryoelectron microscopy as a complex data source for the most of the system biology branches, including the most perspective non-local approaches known as "localomics" and…

Subcellular Processes · Quantitative Biology 2015-01-20 Oleg V. Gradov , Margaret A. Gradova

High-throughput label-free measurements of the optical and mechanical properties of single microparticles play an important role in biological research, drug development, and related large population assays. Mechanical detection techniques…

Instrumentation and Detectors · Physics 2018-12-05 Kewen Han , Jeewon Suh , Gaurav Bahl

The reproducibility of scientific research has become a point of critical concern. We argue that openness and transparency are critical for reproducibility, and we outline an ecosystem for open and transparent science that has emerged…

Computers and Society · Computer Science 2018-09-27 Russell A. Poldrack , Krzysztof J. Gorgolewski , Gael Varoquaux

Driven by the recent rapid increase in the number of materials databases published (open and commercial), I discuss here some perspectives on the growing need for standardized, interoperable, open databases. The field of computational…

Materials Science · Physics 2019-11-14 François-Xavier Coudert

We report a technique based upon the cavity ringdown method that enables to characterize the Brillouin gain coefficient directly in a laser cavity. Material gain, optical cavity parameters and lasing properties can be extracted from…

Optics · Physics 2019-06-24 Ananthu Sebastian , Stéphane Trebaol , Pascal Besnard

Optical microscopy is an indispensable tool in life sciences research, but conventional techniques require compromises between imaging parameters like speed, resolution, field-of-view, and phototoxicity. To overcome these limitations,…

Quantitative Methods · Quantitative Biology 2024-04-01 Leonor Morgado , Estibaliz Gómez-de-Mariscal , Hannah S. Heil , Ricardo Henriques

We introduce the concept of Brillouin optomechanics, a phonon-photon interaction process mediated by the electrostrictive force exerted by light on dielectrics and the photoelastic scattering of light from an acoustic wave. We first provide…

Optics · Physics 2013-09-12 Gaurav Bahl , Tal Carmon

For researchers in electromyography (EMG), and similar biosginals, signal processing is naturally an essential topic. There are a number of excellent tools available. To these one may add the freely available open source statistical…

Data Analysis, Statistics and Probability · Physics 2015-03-19 Frank Borg

Here we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis…

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

With the acceleration of urbanization and living standards, microorganisms play increasingly important roles in industrial production, bio-technique, and food safety testing. Microorganism biovolume measurements are one of the essential…

Computer Vision and Pattern Recognition · Computer Science 2022-05-03 Jiawei Zhang , Chen Li , Md Mamunur Rahaman , Yudong Yao , Pingli Ma , Jinghua Zhang , Xin Zhao , Tao Jiang , Marcin Grzegorzek

Freestanding thin films, a class of low-dimensional materials capable of maintaining structural integrity without substrates, have emerged as a forefront research focus. Their unique advantages-circumventing substrate clamping, liberating…

Materials Science · Physics 2026-01-26 Li Liu , Peixin Qin , Guojian Zhao , Zhiyuan Duan , Jingyu Li , Sixu Jiang , Xiaoyang Tan , Xiaoning Wang , Ziang Meng , Zhiqi Liu

In the last few years, image processing researchers spent a substantial amount of time and effort developing and perfecting image quality assessment algorithms. Bright-field microscopy, for example, produces images whose quality is a…

Image and Video Processing · Electrical Eng. & Systems 2020-03-26 V. A. A. Catanante , O. M. Bruno , J. E. S. Batista Neto

Optical imaging technologies are central to discovery in the life and physical sciences, yet their impact depends on how readily they can be built, adapted, and sustained across laboratories. Digital fabrication, including desktop 3D…

The application of microscopy in biomedical research has come a long way since Antonie van Leeuwenhoek discovered unicellular organisms. Countless innovations have positioned light microscopy as a cornerstone of modern biology and a method…

For over two decades, image-based profiling has revolutionized cell phenotype analysis. Image-based profiling processes rich, high-throughput, microscopy data into thousands of unbiased measurements that reveal phenotypic patterns powerful…