Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective Plane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardware and software platform is shared with the scientific community through a public website, thereby making light sheet microscopy accessible for widespread use and optimization to various applications.
Cite
@article{arxiv.1302.1987,
title = {OpenSPIM - an open access platform for light sheet microscopy},
author = {Peter Pitrone and Johannes Schindelin and Luke Stuyvenberg and Stephan Preibisch and Michael Weber and Kevin W. Eliceiri and Jan Huisken and Pavel Tomancak},
journal= {arXiv preprint arXiv:1302.1987},
year = {2013}
}
Comments
7 pages, 3 figures, 6 supplementary videos, submitted to Nature Methods, associated public website http://openspim.org