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Many essential cellular functions depend on the viscoelastic properties of the cytoplasm. While techniques such as optical tweezers and atomic force microscopy can measure these properties, their reliance on localized probes prevents…

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Optical hyperspectral imaging based on absorption and scattering of photons at the visible and adjacent frequencies denotes one of the most informative and inclusive characterization methods in material research. Unfortunately, restricted…

Focusing using conventional lenses relies on the collection and interference of propagating waves, but discounts the evanescent waves that decay rapidly from the source. Since these evanescent waves contain the finest details of the source,…

Optics · Physics 2013-03-29 Ashwin K. Iyer , G. V. Eleftheriades

Optical scattering presents a major obstacle to high resolution imaging in biological tissue and other turbid media. Conventional photoacoustic imaging can partially overcome this obstacle, enabling imaging of optical absorption in the…

To overcome the limit of diffraction while achieving the superresolution technique, solid immersion lenses are the key optical elements for data storage and nanophotonics applications. Recent demonstrations have shown how different…

Optics · Physics 2026-02-24 Subhankar Roy , Jianping Hu , M Ummal Momeen

Despite the tremendous progresses in wavefront control through or inside complex scattering media, several limitations prevent reaching practical feasibility for nonlinear imaging in biological tissues. While the optimization of nonlinear…

Optics · Physics 2016-12-06 Hilton B. de Aguiar , Sylvain Gigan , Sophie Brasselet

The differentiation between pathological subtypes of non-small cell lung cancer (NSCLC) is an essential step in guiding treatment options and prognosis. However, current clinical practice relies on multi-step staining and labelling…

Quantitative Methods · Quantitative Biology 2026-03-10 Zhenya Zang , David A Dorward , Katherine E Quiohilag , Andrew DJ Wood , James R Hopgood , Ahsan R Akram , Qiang Wang

Obtaining pixel-level annotations in the medical domain is both expensive and time-consuming, often requiring close collaboration between clinical experts and developers. Semi-supervised medical image segmentation aims to leverage limited…

Computer Vision and Pattern Recognition · Computer Science 2025-07-23 Lin Xi , Yingliang Ma , Cheng Wang , Sandra Howell , Aldo Rinaldi , Kawal S. Rhode

Visualizing the details of different cellular structures is of great importance to elucidate cellular functions. However, it is challenging to obtain high quality images of different structures directly due to complex cellular environments.…

Image and Video Processing · Electrical Eng. & Systems 2019-10-01 Yi Liu , Hao Yuan , Zhengyang Wang , Shuiwang Ji

The ability to know what is hidden around a corner or behind a wall provides a crucial advantage when physically going around the obstacle is impossible or dangerous. Previous solutions to this challenge were constrained e.g. by their…

Reflective ptychography is a promising lensless imaging technique with a wide field of view, offering significant potential for applications in semiconductor manufacturing and detection. However, many semiconductor materials are coated with…

Optics · Physics 2025-01-03 Yun Gao , Qijun You , Peixiang Lu , Wei Cao

Scanning electron microscopy (SEM) is indispensable in diverse applications ranging from microelectronics to food processing because it provides large depth-of-field images with a resolution beyond the optical diffraction limit. However,…

We propose a nonlinear imaging scheme with undetected photons that overcomes the diffraction limit by transferring near-field information at one wavelength to far-field information of a correlated photon with a different wavelength…

Quantum Physics · Physics 2022-05-11 Elkin A. Santos , Thomas Pertsch , Frank Setzpfandt , Sina Saravi

Total internal reflection fluorescence microscopy (TIRF) has enabled low-background, live-cell friendly imaging of cell surfaces and other thin samples thanks to the shallow penetration of the evanescent light field into the sample. The…

Image Phase Alignment Super-Sampling (ImPASS) is a computational imaging algorithm for converting a sequence of displaced low-resolution images into a single high-resolution image. The method consists of a unique combination of Phase…

Optics · Physics 2025-09-01 James N. Caron

Spatial resolution of most imaging devices is fundamentally restricted by diffraction. This limitation is manifested in the loss of high spatial frequency information contained in evanescent waves. As a result, conventional far-field optics…

Optics · Physics 2010-03-16 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

Non-Line-of-Sight (NLOS) imaging allows to observe objects partially or fully occluded from direct view, by analyzing indirect diffuse reflections off a secondary, relay surface. Despite its many potential applications, existing methods…

Computer Vision and Pattern Recognition · Computer Science 2019-08-07 Xiaochun Liu , Ibón Guillén , Marco La Manna , Ji Hyun Nam , Syed Azer Reza , Toan Huu Le , Diego Gutierrez , Adrian Jarabo , Andreas Velten

Ground-based imaging systems struggle to achieve diffraction-limited resolution when atmospheric turbulence and photon scarcity act simultaneously. In this regime, conventional adaptive optics, speckle imaging, and blind deconvolution lack…

Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

Computer Vision and Pattern Recognition · Computer Science 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan