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Nonlinear optical (NLO) imaging platforms traditionally rely on refractive microscope objectives, which suffer from chromatic aberrations and temporal dispersion of pulsed excitation light. These issues degrade spatial imaging properties…

Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of…

Optics · Physics 2013-05-30 Osip Schwartz , Dan Oron

Seeing and recognizing an object whose size is much smaller than the illumination wavelength is a challenging task for an observer placed in the far field, due to the diffraction limit. Recent advances in near and far field microscopy have…

Applied Physics · Physics 2020-08-19 Bakhtiyar Orazbayev , Romain Fleury

Histopathological visualizations are a pillar of modern medicine and biological research. Surgical oncology relies exclusively on post-operative histology to determine definitive surgical success and guide adjuvant treatments. The current…

Image and Video Processing · Electrical Eng. & Systems 2022-04-04 Marian Boktor , Benjamin Ecclestone , Vlad Pekar , Deepak Dinakaran , John R. Mackey , Paul Fieguth , Parsin Haji Reza

Virtual staining of tissue offers a powerful tool for transforming label-free microscopy images of unstained tissue into equivalents of histochemically stained samples. This study presents a diffusion model-based super-resolution virtual…

Image and Video Processing · Electrical Eng. & Systems 2025-07-01 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Hanlong Chen , Aydogan Ozcan

Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent…

Quantitative Methods · Quantitative Biology 2020-06-24 Martin Oheim , Adi Salomon , Maia Brunstein

Advancements in fast electron detectors have enabled the statistically significant sampling of crystal structures on the nanometre scale by means of Scanning Electron Nanobeam Diffraction (SEND). Characterisation of structural similarity…

Materials Science · Physics 2022-07-28 Andy Bridger , William I. F. David , Thomas J. Wood , Mohsen Danaie , Keith T. Butler

Recently, it was discovered that microsphere can generate super-resolution focusing beyond diffraction limit. This has led to the development of an exciting super-resolution imaging technique -microsphere nanoscopy- that features a record…

Optics · Physics 2021-08-26 Zengbo Wang

Until recently, conventional biochemical staining had the undisputed status as well-established benchmark for most biomedical problems related to clinical diagnostics, fundamental research and biotechnology. Despite this role as…

We propose the Subwavelength Localization via Adiabatic Passage (SLAP) technique to coherently achieve state-selective patterning of matter waves well beyond the diffraction limit. The SLAP technique consists in coupling two partially…

Quantum Physics · Physics 2015-05-13 J. Mompart , V. Ahufinger , G. Birkl

Label-free observation of nanoparticles by far-field optical microscopy is challenging because their ability to scatter or absorb light dramatically diminishes with decreasing size. Surface plasmon resonance (SPR) and localized surface…

Optics · Physics 2020-12-02 Nareg Ohannesian , Ibrahim Misbah , Steven H Lin , Wei-Chuan Shih

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

Recently it has been proposed that a planar slab of material, for which both the permittivity and permeability have the values of -1, could bring not only the propagating fields associated with a source to a focus, but could also refocus…

Condensed Matter · Physics 2009-11-07 D. R. Smith , D. Schurig , Marshall Rosenbluth , S. Schultz , S. Anantha Ramakrishna , J. B. Pendry

Super-resolution imaging techniques enable nanoscale microscopy in fields such as physics, biology, and chemistry. However, many super-resolution techniques require specialized optical components, such as a helical-phase mask. We present a…

We report the observation of imaging beyond the diffraction limit due to bound surface states in negative refraction photonic crystals. We achieve an effective negative index figure-of-merit [-Re(n)/Im(n)] of at least 380, ~125x improvement…

In biological microscopy, light scattering represents the main limitation to image at depth. Recently, a set of wavefront shaping techniques has been developed in order to manipulate coherent light in strongly disordered materials. The…

Optics · Physics 2021-01-07 Antoine Boniface , Jonathan Dong , Sylvain Gigan

A common class of problems in remote sensing is scene classification, a fundamentally important task for natural hazards identification, geographic image retrieval, and environment monitoring. Recent developments in this field rely…

Computer Vision and Pattern Recognition · Computer Science 2022-01-21 Suhas Kotha , Anirudh Koul , Siddha Ganju , Meher Kasam

Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of…

Quantitative Methods · Quantitative Biology 2024-03-29 Yow-Ren Chang , Seong-Min Kim , Young Jong Lee

A lensless digital holography enables wide-field microscopic imaging without the limitations imposed by optical lens performance. However, conventional holographic imaging often relies on magnifying optical systems to compensate for the low…

Optics · Physics 2025-06-24 Byung Gyu Chae

Understanding the biophysical and biochemical properties of molecular nanocarriers under physiological conditions and with minimal interference is crucial for advancing nanomedicine, photopharmacology, drug delivery, nanotheranostics and…

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