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Prostate cancer (PCa) is graded by pathologists by examining the architectural pattern of cancerous epithelial tissue on hematoxylin and eosin (H&E) stained slides. Given the importance of gland morphology, automatically differentiating…

Structured Illumination Microscopy (SIM) enables rapid, high-contrast optical sectioning of fresh tissue without staining or physical sectioning, making it promising for intraoperative and point-of-care diagnostics. Recent foundation and…

In certain types of cancerous tissue, mitotic count has been shown to be associated with tumor proliferation, poor prognosis, and therapeutic resistance. Due to the high inter-rater variability of mitotic counting by pathologists,…

Computer Vision and Pattern Recognition · Computer Science 2023-10-12 Ruiwen Ding , James Hall , Neil Tenenholtz , Kristen Severson

Digital pathology and microscopy image analysis are widely employed in the segmentation of digitally scanned IHC slides, primarily to identify cancer and pinpoint regions of interest (ROI) indicative of tumor presence. However, current ROI…

Computer Vision and Pattern Recognition · Computer Science 2024-06-11 Akash Modi , Sumit Kumar Jha , Purnendu Mishra , Rajiv Kumar , Kiran Aatre , Gursewak Singh , Shubham Mathur

Registration of serial whole-slide histopathological images (WSIs) is critical for enabling direct comparison across diverse stains and for preparing paired datasets in artificial intelligence (AI) workflows such as virtual staining and…

Computer Vision and Pattern Recognition · Computer Science 2025-09-04 Zeyu Liu , Shengwei Ding

Quantitative analysis of cell structures is essential for biomedical and pharmaceutical research. The standard imaging approach relies on fluorescence microscopy, where cell structures of interest are labeled by chemical staining…

Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

Stain color variation in histological images, caused by a variety of factors, is a challenge not only for the visual diagnosis of pathologists but also for cell segmentation algorithms. To eliminate the color variation, many stain…

Image and Video Processing · Electrical Eng. & Systems 2022-10-27 Huaqian Wu , Nicolas Souedet , Camille Mabillon , Caroline Jan , Cédric Clouchoux , Thierry Delzescaux

The quantification of biomarkers on immunohistochemistry breast cancer images is essential for defining appropriate therapy for breast cancer patients, as well as for extracting relevant information on disease prognosis. This is an arduous…

Image and Video Processing · Electrical Eng. & Systems 2023-11-27 Blanca Maria Priego-Torresa , Barbara Lobato-Delgado , Lidia Atienza-Cuevas , Daniel Sanchez-Morillo

Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

Computer Vision and Pattern Recognition · Computer Science 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan

Mitotic figure count is an important marker of tumor proliferation and has been shown to be associated with patients' prognosis. Deep learning based mitotic figure detection methods have been utilized to automatically locate the cell in…

Computer Vision and Pattern Recognition · Computer Science 2021-09-01 Jingtang Liang , Cheng Wang , Yujie Cheng , Zheng Wang , Fang Wang , Liyu Huang , Zhibin Yu , Yubo Wang

We propose a Deep learning-based weak label learning method for analyzing whole slide images (WSIs) of Hematoxylin and Eosin (H&E) stained tumor tissue not requiring pixel-level or tile-level annotations using Self-supervised pre-training…

Image and Video Processing · Electrical Eng. & Systems 2023-06-29 Yoni Schirris , Efstratios Gavves , Iris Nederlof , Hugo Mark Horlings , Jonas Teuwen

High-fidelity registration of histopathological whole slide images (WSIs), such as hematoxylin & eosin (H&E) and immunohistochemistry (IHC), is vital for integrated molecular analysis but challenging to evaluate without ground-truth (GT)…

Computer Vision and Pattern Recognition · Computer Science 2026-02-05 Shikha Dubey , Patricia Raciti , Kristopher Standish , Albert Juan Ramon , Erik Ames Burlingame

The accurate classification of lymphoma subtypes using hematoxylin and eosin (H&E)-stained tissue is complicated by the wide range of morphological features these cancers can exhibit. We present LymphoML - an interpretable machine learning…

In recent years, deep neural networks (DNNs) have demonstrated remarkable performance in pathology applications, potentially even outperforming expert pathologists due to their ability to learn subtle features from large datasets. One…

Image and Video Processing · Electrical Eng. & Systems 2024-09-16 Siyu , Lin , Haowen Zhou , Richard J. Cote , Mark Watson , Ramaswamy Govindan , Changhuei Yang

The differentiation between pathological subtypes of non-small cell lung cancer (NSCLC) is an essential step in guiding treatment options and prognosis. However, current clinical practice relies on multi-step staining and labelling…

Quantitative Methods · Quantitative Biology 2026-03-10 Zhenya Zang , David A Dorward , Katherine E Quiohilag , Andrew DJ Wood , James R Hopgood , Ahsan R Akram , Qiang Wang

Immunohistochemistry (IHC) staining plays a significant role in the evaluation of diseases such as breast cancer. The H&E-to-IHC transformation based on generative models provides a simple and cost-effective method for obtaining IHC images.…

Computer Vision and Pattern Recognition · Computer Science 2025-02-28 Yifan Jia , Xingda Yu , Zhengyang Ji , Songning Lai , Yutao Yue

Deep learning models that are trained on histopathological images obtained from a single lab and/or scanner give poor inference performance on images obtained from another scanner/lab with a different staining protocol. In recent years,…

Image and Video Processing · Electrical Eng. & Systems 2020-10-07 Harshal Nishar , Nikhil Chavanke , Nitin Singhal

Recently, various deep learning methods have shown significant successes in medical image analysis, especially in the detection of cancer metastases in hematoxylin and eosin (H&E) stained whole-slide images (WSIs). However, in order to…

Image and Video Processing · Electrical Eng. & Systems 2023-03-21 Yinsheng He , Xingyu Li

The analysis of the tumor environment on digital histopathology slides is becoming key for the understanding of the immune response against cancer, supporting the development of novel immuno-therapies. We introduce here a novel deep…

Image and Video Processing · Electrical Eng. & Systems 2019-06-27 Ansh Kapil , Tobias Wiestler , Simon Lanzmich , Abraham Silva , Keith Steele , Marlon Rebelatto , Guenter Schmidt , Nicolas Brieu