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Whole Slide Images (WSIs) in digital pathology are used to diagnose cancer subtypes. The difference in procedures to acquire WSIs at various trial sites gives rise to variability in the histopathology images, thus making consistent…

Image and Video Processing · Electrical Eng. & Systems 2022-04-07 Milad Sikaroudi , Shahryar Rahnamayan , H. R. Tizhoosh

Virtual staining leverages computer-aided techniques to transfer the style of histochemically stained tissue samples to other staining types. In virtual staining of pathological images, maintaining strict structural consistency is crucial,…

Image and Video Processing · Electrical Eng. & Systems 2024-12-17 Bing Xiong , Yue Peng , RanRan Zhang , Fuqiang Chen , JiaYe He , Wenjian Qin

Accurate and fast histological staining is crucial in histopathology, impacting diagnostic precision and reliability. Traditional staining methods are time-consuming and subjective, causing delays in diagnosis. Digital pathology plays a…

Image and Video Processing · Electrical Eng. & Systems 2023-07-06 Marian Boktor , James E. D. Tweel , Benjamin R. Ecclestone , Jennifer Ai Ye , Paul Fieguth , Parsin Haji Reza

Purpose: This work addresses the detection of Helicobacter pylori (H. pylori) in histological images with immunohistochemical staining. This analysis is a time demanding task, currently done by an expert pathologist that visually inspects…

Image and Video Processing · Electrical Eng. & Systems 2024-12-19 Pau Cano , Eva Musulen , Debora Gil

Histopathological visualizations are a pillar of modern medicine and biological research. Surgical oncology relies exclusively on post-operative histology to determine definitive surgical success and guide adjuvant treatments. The current…

Image and Video Processing · Electrical Eng. & Systems 2022-04-04 Marian Boktor , Benjamin Ecclestone , Vlad Pekar , Deepak Dinakaran , John R. Mackey , Paul Fieguth , Parsin Haji Reza

Due to its superior efficiency in utilizing annotations and addressing gigapixel-sized images, multiple instance learning (MIL) has shown great promise as a framework for whole slide image (WSI) classification in digital pathology…

Quantitative Methods · Quantitative Biology 2023-07-14 Qiehe Sun , Jiawen Li , Jin Xu , Junru Cheng , Tian Guan , Yonghong He

Masked image modelling (MIM) is a powerful self-supervised representation learning paradigm, whose potential has not been widely demonstrated in medical image analysis. In this work, we show the capacity of MIM to capture rich semantic…

Computer Vision and Pattern Recognition · Computer Science 2023-06-30 Piotr Wójcik , Hussein Naji , Adrian Simon , Reinhard Büttner , Katarzyna Bożek

The application of supervised deep learning methods in digital pathology is limited due to their sensitivity to domain shift. Digital Pathology is an area prone to high variability due to many sources, including the common practice of…

Image and Video Processing · Electrical Eng. & Systems 2020-12-24 Jelica Vasiljević , Friedrich Feuerhake , Cédric Wemmert , Thomas Lampert

Color inconsistency is an inevitable challenge in computational pathology, which generally happens because of stain intensity variations or sections scanned by different scanners. It harms the pathological image analysis methods, especially…

Image and Video Processing · Electrical Eng. & Systems 2022-03-01 Bingchao Zhao , Jiatai Lin , Changhong Liang , Zongjian Yi , Xin Chen , Bingbing Li , Weihao Qiu , Danyi Li , Li Liang , Chu Han , Zaiyi Liu

Immunohistochemistry is a valuable diagnostic tool for cancer pathology. However, it requires specialist labs and equipment, is time-intensive, and is difficult to reproduce. Consequently, a long term aim is to provide a digital method of…

Image and Video Processing · Electrical Eng. & Systems 2022-04-15 Christopher D. Walsh , Joanne Edwards , Robert H. Insall

The detection of nuclei is one of the most fundamental components of computational pathology. Current state-of-the-art methods are based on deep learning, with the prerequisite that extensive labeled datasets are available. The increasing…

Image and Video Processing · Electrical Eng. & Systems 2019-07-11 Nicolas Brieu , Armin Meier , Ansh Kapil , Ralf Schoenmeyer , Christos G. Gavriel , Peter D. Caie , Günter Schmidt

Hematoxylin and Eosin (H&E) staining is a widely used sample preparation procedure for enhancing the saturation of tissue sections and the contrast between nuclei and cytoplasm in histology images for medical diagnostics. However, various…

Image and Video Processing · Electrical Eng. & Systems 2023-07-18 Fangda Li , Zhiqiang Hu , Wen Chen , Avinash Kak

Virtual staining, or in-silico-labeling, has been proposed to computationally generate synthetic fluorescence images from label-free images by use of deep learning-based image-to-image translation networks. In most reported studies,…

Image and Video Processing · Electrical Eng. & Systems 2025-08-04 Sourya Sengupta , Jianquan Xu , Phuong Nguyen , Frank J. Brooks , Yang Liu , Mark A. Anastasio

In digital pathology, different staining procedures and scanners cause substantial color variations in whole-slide images (WSIs), especially across different laboratories. These color shifts result in a poor generalization of deep…

Image and Video Processing · Electrical Eng. & Systems 2021-07-27 Sophia J. Wagner , Nadieh Khalili , Raghav Sharma , Melanie Boxberg , Carsten Marr , Walter de Back , Tingying Peng

Histopathological imaging is vital for cancer research and clinical practice, with multiplexed Immunofluorescence (MxIF) and Hematoxylin and Eosin (H&E) providing complementary insights. However, aligning different stains at the cell level…

Image and Video Processing · Electrical Eng. & Systems 2024-10-02 Jun Jiang , Raymond Moore , Brenna Novotny , Leo Liu , Zachary Fogarty , Ray Guo , Markovic Svetomir , Chen Wang

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images…

Image and Video Processing · Electrical Eng. & Systems 2019-02-08 Yair Rivenson , Tairan Liu , Zhensong Wei , Yibo Zhang , Aydogan Ozcan

The integrity of the reconstructed human epidermis generated in vitro could be assessed using histological analyses combined with immunohistochemical staining of keratinocyte differentiation markers. Computer-based analysis of scanned…

Image and Video Processing · Electrical Eng. & Systems 2025-12-01 Dawid Zamojski , Agnieszka Gogler , Dorota Scieglinska , Michal Marczyk

Current methods for diagnosing the progression of multiple types of cancer within patients rely on interpreting stained needle biopsies. This process is time-consuming and susceptible to error throughout the paraffinization, Hematoxylin and…

Image and Video Processing · Electrical Eng. & Systems 2022-04-19 Benjamin Paulson , Theodore Colwell , Natalia Bukowski , Joseph Weller , Andrew Crisler , John Cisler , Alexander Drobek , Alexander Neuwirth

Prostate cancer diagnosis heavily relies on histopathological evaluation, which is subject to variability. While immunohistochemical staining (IHC) assists in distinguishing benign from malignant tissue, it involves increased work, higher…

Traditional staining normalization approaches, e.g. Macenko, typically rely on the choice of a single representative reference image, which may not adequately account for the diverse staining patterns of datasets collected in practical…

Image and Video Processing · Electrical Eng. & Systems 2024-06-11 Desislav Ivanov , Carlo Alberto Barbano , Marco Grangetto
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