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Multiple instance learning (MIL) is the dominant framework for whole-slide image analysis in computational pathology, typically combining a frozen patch encoder, a projection layer, and a slide-level aggregator. While encoders and…

Quantitative Methods · Quantitative Biology 2026-05-19 Yucheng Xing , Pei Liu , Jingying Ma , Ruping Hong , Jiangdong Qiu , Tianyu Liu , Kai He , Ling Huang , Mengling Feng

In this paper, we develop a complete pipeline for stain normalization, segmentation, and classification of nuclei in hematoxylin and eosin (H&E) stained breast cancer histopathology images. In the first step, we use a CNN-based stain…

Computer Vision and Pattern Recognition · Computer Science 2018-11-12 Edwin Yuan , Junkyo Suh

Hematoxylin and Eosin (H&E) stained Whole Slide Images (WSIs) are utilized for biopsy visualization-based diagnostic and prognostic assessment of diseases. Variation in the H&E staining process across different lab sites can lead to…

Image and Video Processing · Electrical Eng. & Systems 2020-11-24 Aman Shrivastava , Will Adorno , Yash Sharma , Lubaina Ehsan , S. Asad Ali , Sean R. Moore , Beatrice C. Amadi , Paul Kelly , Sana Syed , Donald E. Brown

The count of mitotic figures (MFs) observed in hematoxylin and eosin (H&E)-stained slides is an important prognostic marker as it is a measure for tumor cell proliferation. However, the identification of MFs has a known low inter-rater…

Computational pathology is a domain that aims to develop algorithms to automatically analyze large digitized histopathology images, called whole slide images (WSI). WSIs are produced scanning thin tissue samples that are stained to make…

Image and Video Processing · Electrical Eng. & Systems 2022-01-20 Niccoló Marini , Manfredo Atzori , Sebastian Otálora , Stephane Marchand-Maillet , Henning Müller

Histological staining is the gold standard for tissue examination in clinical pathology and life-science research, which visualizes the tissue and cellular structures using chromatic dyes or fluorescence labels to aid the microscopic…

Medical Physics · Physics 2023-03-07 Bijie Bai , Xilin Yang , Yuzhu Li , Yijie Zhang , Nir Pillar , Aydogan Ozcan

In digital pathology, many image analysis tasks are challenged by the need for large and time-consuming manual data annotations to cope with various sources of variability in the image domain. Unsupervised domain adaptation based on…

Image and Video Processing · Electrical Eng. & Systems 2022-05-18 Nassim Bouteldja , Barbara Mara Klinkhammer , Tarek Schlaich , Peter Boor , Dorit Merhof

Image-to-image translation (I2I) methods allow the generation of artificial images that share the content of the original image but have a different style. With the advances in Generative Adversarial Networks (GANs)-based methods, I2I…

Image and Video Processing · Electrical Eng. & Systems 2023-04-07 Igor Zingman , Sergio Frayle , Ivan Tankoyeu , Segrey Sukhanov , Fabian Heinemann

Expression of human epidermal growth factor receptor 2 (HER2) is an important biomarker in breast cancer patients who can benefit from cost-effective automatic Hematoxylin and Eosin (H\&E) HER2 scoring. However, developing such scoring…

Computer Vision and Pattern Recognition · Computer Science 2024-11-11 Rawan S. Abdulsadig , Bryan M. Williams , Nikolay Burlutskiy

The presence and density of specific types of immune cells are important to understand a patient's immune response to cancer. However, immunofluorescence staining required to identify T cell subtypes is expensive, time-consuming, and rarely…

Computer Vision and Pattern Recognition · Computer Science 2022-10-18 Georg Wölflein , In Hwa Um , David J Harrison , Ognjen Arandjelović

Immunohistochemical (IHC) virtual staining is a task that generates virtual IHC images from H\&E images while maintaining pathological semantic consistency with adjacent slices. This task aims to achieve cross-domain mapping between…

Computer Vision and Pattern Recognition · Computer Science 2026-05-20 Yue Peng , Bing Xiong , Fuqiang Chen , De Eybo , RanRan Zhang , Wanming Hu , Jing Cai , Wenjian Qin

Melanoma is one of the most aggressive forms of skin cancer, causing a large proportion of skin cancer deaths. However, melanoma diagnoses by pathologists shows low interrater reliability. As melanoma is a cancer of the melanocyte, there is…

Quantitative Methods · Quantitative Biology 2024-03-15 Mikio Tada , Ursula E. Lang , Iwei Yeh , Elizabeth S. Keiser , Maria L. Wei , Michael J. Keiser

Three-dimensional (3D) histopathology of unprocessed tissues has the potential to transform disease management by enabling volumetric characterization of tissue microarchitecture and in-vivo assessment. Back-illumination Interference…

Computer Vision and Pattern Recognition · Computer Science 2026-05-22 Anthony Song , Boyan Zhou , Mayank Golhar , Marisa Morakis , Alex Baras , Nicholas Durr

Histopathology relies on the analysis of microscopic tissue images to diagnose disease. A crucial part of tissue preparation is staining whereby a dye is used to make the salient tissue components more distinguishable. However, differences…

Image and Video Processing · Electrical Eng. & Systems 2022-08-03 Haseeb Nazki , Ognjen Arandjelović , InHwa Um , David Harrison

Multiplex immunofluorescence (mIF) enables simultaneous single-cell quantification of multiple biomarkers within intact tissue architecture, yet its high reagent cost, multi-round staining protocols, and need for specialized imaging…

Computer Vision and Pattern Recognition · Computer Science 2026-03-18 Junhyeok Lee , Han Jang , Heeseong Eum , Joon Jang , Kyu Sung Choi

Histopathology slides are routinely marked by pathologists using permanent ink markers that should not be removed as they form part of the medical record. Often tumour regions are marked up for the purpose of highlighting features or other…

Computer Vision and Pattern Recognition · Computer Science 2019-05-14 Sharib Ali , Nasullah Khalid Alham , Clare Verrill , Jens Rittscher

Different types of staining highlight different structures in organs, thereby assisting in diagnosis. However, due to the impossibility of repeated staining, we cannot obtain different types of stained slides of the same tissue area.…

Image and Video Processing · Electrical Eng. & Systems 2024-04-17 Zexin Li , Yiyang Lin , Zijie Fang , Shuyan Li , Xiu Li

Stains are essential in histopathology to visualize specific tissue characteristics, with Haematoxylin and Eosin (H&E) serving as the clinical standard. However, pathologists frequently utilize a variety of special stains for the diagnosis…

Computer Vision and Pattern Recognition · Computer Science 2026-02-11 Oskar Thaeter , Christian Grashei , Anette Haas , Elisa Schmoeckel , Han Li , Peter J. Schüffler

Virtual staining of histopathology images (e.g., H&E-IHC) is an emerging tool in digital pathology, enabling faster and cheaper workflows by synthesizing target stains from routinely acquired slides. Yet, the quality of virtual staining…

Computer Vision and Pattern Recognition · Computer Science 2026-05-21 Fedor Gubanov , Svetlana Illarionova , Vlad Kozlovskiy , Mikhail Romanov , Yersultan Akhmetov , Aida Akaeva , Vyacheslav Grinevich , Rifat Hamoudi , Maxim Sharaev

Increased levels of tumor infiltrating lymphocytes (TILs) in cancer tissue indicate favourable outcomes in many types of cancer. Manual quantification of immune cells is inaccurate and time consuming for pathologists. Our aim is to leverage…

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