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Immunohistochemistry (IHC) is essential for assessing specific immune biomarkers like Human Epidermal growth-factor Receptor 2 (HER2) in breast cancer. However, the traditional protocols of obtaining IHC stains are resource-intensive,…

Image and Video Processing · Electrical Eng. & Systems 2026-04-10 Aasim Bin Saleem , Amr Ahmed , Ardhendu Behera , Hafeezullah Amin , Iman Yi Liao , Mahmoud Khattab , Pan Jia Wern , Haslina Makmur

Hematoxylin and Eosin (H&E) staining is widely regarded as the standard in pathology for diagnosing diseases and tracking tumor recurrence. While H&E staining shows tissue structures, it lacks the ability to reveal specific proteins that…

Image and Video Processing · Electrical Eng. & Systems 2025-05-21 Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

Hematoxylin and eosin (H&E) staining visualizes histology but lacks specificity for diagnostic markers. Immunohistochemistry (IHC) staining provides protein-targeted staining but is restricted by tissue availability and antibody…

Image and Video Processing · Electrical Eng. & Systems 2025-07-01 Anran Liu , Xiaofei Wang , Jing Cai , Chao Li

Hematoxylin and Eosin (H&E) staining is a cornerstone of pathological analysis, offering reliable visualization of cellular morphology and tissue architecture for cancer diagnosis, subtyping, and grading. Immunohistochemistry (IHC) staining…

Image and Video Processing · Electrical Eng. & Systems 2025-06-23 Amit Das , Naofumi Tomita , Kyle J. Syme , Weijie Ma , Paige O'Connor , Kristin N. Corbett , Bing Ren , Xiaoying Liu , Saeed Hassanpour

Compared to hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC) not only maintains the structural features of tissue samples, but also provides high-resolution protein localization, which is essential for aiding in pathology…

Computer Vision and Pattern Recognition · Computer Science 2025-11-04 Yuhang Kang , Ziyu Su , Tianyang Wang , Zaibo Li , Wei Chen , Muhammad Khalid Khan Niazi

Virtual immunohistochemistry (IHC) staining from hematoxylin and eosin (H&E) images can accelerate diagnostics by providing preliminary molecular insight directly from routine sections, reducing the need for repeat sectioning when tissue is…

Computer Vision and Pattern Recognition · Computer Science 2026-03-16 Jillur Rahman Saurav , Thuong Le Hoai Pham , Pritam Mukherjee , Paul Yi , Brent A. Orr , Jacob M. Luber

Computational analysis of multiplexed immunofluorescence histology data is emerging as an important method for understanding the tumour micro-environment in cancer. This work presents HEMIT, a dataset designed for translating Hematoxylin…

Image and Video Processing · Electrical Eng. & Systems 2024-09-30 Chang Bian , Beth Philips , Tim Cootes , Martin Fergie

Hematoxylin and eosin (H&E) staining is the clinical standard for assessing tissue morphology, but it lacks molecular-level diagnostic information. In contrast, immunohistochemistry (IHC) provides crucial insights into biomarker expression,…

Image and Video Processing · Electrical Eng. & Systems 2025-08-05 Jingsong Liu , Xiaofeng Deng , Han Li , Azar Kazemi , Christian Grashei , Gesa Wilkens , Xin You , Tanja Groll , Nassir Navab , Carolin Mogler , Peter J. Schüffler

The diagnosis of cancer is mainly performed by visual analysis of the pathologists, through examining the morphology of the tissue slices and the spatial arrangement of the cells. If the microscopic image of a specimen is not stained, it…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Pegah Salehi , Abdolah Chalechale

Immunohistochemical (IHC) staining serves as a valuable technique for detecting specific antigens or proteins through antibody-mediated visualization. However, the IHC staining process is both time-consuming and costly. To address these…

Computer Vision and Pattern Recognition · Computer Science 2025-04-02 Zetong Chen , Yuzhuo Chen , Hai Zhong , Xu Qiao

Hematoxylin and eosin (H&E)-stained slides are central to cancer diagnosis and monitoring, visualizing tissue architecture and cellular morphology. However, H&E lacks the molecular specificity needed to distinguish cell states and…

Image and Video Processing · Electrical Eng. & Systems 2026-04-14 Tushar Kataria , Beatrice Knudsen , Shireen Y. Elhabian

Multiplex imaging is revolutionizing pathology by enabling the simultaneous visualization of multiple biomarkers within tissue samples, providing molecular-level insights that traditional hematoxylin and eosin (H&E) staining cannot provide.…

Image and Video Processing · Electrical Eng. & Systems 2026-01-06 Hyun-Jic Oh , Junsik Kim , Zhiyi Shi , Yichen Wu , Yu-An Chen , Peter K Sorger , Hanspeter Pfister , Won-Ki Jeong

Virtual staining offers a promising method for converting Hematoxylin and Eosin (H&E) images into Immunohistochemical (IHC) images, eliminating the need for costly chemical processes. However, existing methods often struggle to utilize…

Computer Vision and Pattern Recognition · Computer Science 2025-11-11 Sicheng Yang , Zhaohu Xing , Haipeng Zhou , Lei Zhu

Here, we present a diffusion transformer (DiT)-based pixel super-resolution virtual staining approach to transform low-resolution FTIR microspectroscopic images of the unstained tissues into corresponding high-resolution H&E-stained images.…

Optics · Physics 2026-03-10 Yudong Tian , Xiangyu Zhao , Yuqing Liu , Bofei Yang , Chongzhao Wu

Breast cancer presents a significant healthcare challenge globally, demanding precise diagnostics and effective treatment strategies, where histopathological examination of Hematoxylin and Eosin (H&E) stained tissue sections plays a central…

Image and Video Processing · Electrical Eng. & Systems 2024-08-06 Linhao Qu , Chengsheng Zhang , Guihui Li , Haiyong Zheng , Chen Peng , Wei He

Histological Tertiary Lymphoid Structures (TLSs) are increasingly recognized for their correlation with the efficacy of immunotherapy in various solid tumors. Traditionally, the identification and characterization of TLSs rely on…

Image and Video Processing · Electrical Eng. & Systems 2024-08-27 Qiuli Wang , Yongxu Liu , Li Ma , Xianqi Wang , Wei Chen , Xiaohong Yao

Spatial Transcriptomics (ST) allows a high-resolution measurement of RNA sequence abundance by systematically connecting cell morphology depicted in Hematoxylin and Eosin (H&E) stained histology images to spatially resolved gene…

Computer Vision and Pattern Recognition · Computer Science 2025-03-21 Sichen Zhu , Yuchen Zhu , Molei Tao , Peng Qiu

Hematoxylin and Eosin (H&E) has been the gold standard in tissue analysis for decades, however, tissue specimens stained in different laboratories vary, often significantly, in appearance. This variation poses a challenge for both…

Image and Video Processing · Electrical Eng. & Systems 2025-06-25 Umair Khan , Jouni Härkönen , Marjukka Friman , Leena Latonen , Teijo Kuopio , Pekka Ruusuvuori

Digital pathology has advanced significantly over the last decade, with Whole Slide Images (WSIs) encompassing vast amounts of data essential for accurate disease diagnosis. High-resolution WSIs are essential for precise diagnosis but…

Image and Video Processing · Electrical Eng. & Systems 2024-08-28 Xuan Xu , Saarthak Kapse , Prateek Prasanna

Multiple instance learning (MIL) is the dominant framework for whole-slide image analysis in computational pathology, typically combining a frozen patch encoder, a projection layer, and a slide-level aggregator. While encoders and…

Quantitative Methods · Quantitative Biology 2026-05-19 Yucheng Xing , Pei Liu , Jingying Ma , Ruping Hong , Jiangdong Qiu , Tianyu Liu , Kai He , Ling Huang , Mengling Feng
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