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Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…
This paper reported a general noninterferometric high-accuracy quantitative phase imaging (QPI) method for arbitrary complex valued objects. Given by a typical 4f optical configuration as the imaging system, three frames of small-window…
CDI is a lensless imaging technique that enables atomic-resolution imaging of non-crystalline specimens and their dynamics. However, its broader implementation has been hindered by the instability and ill-posedness of its reconstruction…
Non-contact measurement of the refractive index and thickness of multilayer biological tissues is of great significance for biomedical applications and can greatly improve medical diagnosis and treatment. In this work, we introduce a…
Diffractometric biosensing is a promising technology to overcome critical limitations of refractometric biosensors, the dominant class of label-free optical transducers. These limitations manifest themselves by higher noise and drifts due…
We present a tomographic imaging technique, termed Deep Prior Diffraction Tomography (DP-DT), to reconstruct the 3D refractive index (RI) of thick biological samples at high resolution from a sequence of low-resolution images collected…
Coherent diffractive imaging is a technique that recovers the sample image by numerically inverting its diffraction pattern. We propose a generalization of this method for the inversion of multi-wavelength data. Using this approach, we show…
Phase recovery (PR) refers to calculating the phase of the light field from its intensity measurements. As exemplified from quantitative phase imaging and coherent diffraction imaging to adaptive optics, PR is essential for reconstructing…
We present an analytical description and an experimental realization of interscale mixing microscopy, a diffraction-based imaging technique that is capable of detecting wavelength/10 objects in far-field measurements with both coherent and…
Curved refractive objects are common in the human environment, and have a complex visual appearance that can cause robotic vision algorithms to fail. Light-field cameras allow us to address this challenge by capturing the view-dependent…
Within the last decade microscopic multiphase flows have gained broad interest. An exact understanding of the underlying hydrodynamic interrelations is the key for successful reactor layout and reaction control. To examine the local…
Diffuse Reflectance Spectroscopy (DRS) is a well-established optical technique for tissue composition assessment which has been clinically evaluated for tumour detection to ensure the complete removal of cancerous tissue. While point-wise…
Diffraction tomography is an inverse scattering technique used to reconstruct the spatial distribution of the material properties of a weakly scattering object. The object is exposed to radiation, typically light or ultrasound, and the…
In this chapter, we introduce digital holographic microscopy (DHM) as a marker-free method to determine the refractive index of single, spherical cells in suspension. The refractive index is a conclusive measure in a biological context.…
Reference star differential imaging (RDI) is a powerful strategy for high contrast imaging. Using example observations taken with the vortex coronagraph mode of Keck/NIRC2 in $L^\prime$ band, we demonstrate that RDI provides improved…
Fundamental optics such as lenses and prisms work by applying phase shifts to incoming light via the refractive index. In these macroscopic devices, many particles each contribute a miniscule phase shift, working together to impose a total…
In coherent X-ray diffraction microscopy the diffraction pattern generated by a sample illuminated with coherent x-rays is recorded, and a computer algorithm recovers the unmeasured phases to synthesize an image. By avoiding the use of a…
While the implementation of single particle coherent diffraction imaging for non-crystalline particles is complicated by current limitations in photon flux, hit rate, and sample delivery a concept of many-particle coherent diffraction…
We propose and demonstrate a new phase retrieval method for imaging through random media. Although methods to recover the Fourier amplitude through random distortions are well established, recovery of the Fourier phase has been a more…
Transparent and reflective objects, which are common in our everyday lives, present a significant challenge to 3D imaging techniques due to their unique visual and optical properties. Faced with these types of objects, RGB-D cameras fail to…