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Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Phase is a fundamental resource for optical imaging but cannot be directly observed with intensity measurements. The existing methods to quantify a phase distribution rely on complex devices and structures. Here we experimentally…

Optics · Physics 2020-03-25 Tengfeng Zhu , Junyi Huang , Zhichao Ruan

Quantitative phase imaging (QPI) is often used for label-free single cell analysis with its high-contrast images of optical phase delay (OPD) map. Contrary to other imaging methods, sensitivity improvement has not been intensively explored…

Optics · Physics 2025-05-06 Keiichiro Toda , Miu Tamamitsu , Takuro Ideguchi

Refractive Index Tomography is the inverse problem of reconstructing the continuously-varying 3D refractive index in a scene using 2D projected image measurements. Although a purely refractive field is not directly visible, it bends light…

Computer Vision and Pattern Recognition · Computer Science 2023-12-06 Brandon Zhao , Aviad Levis , Liam Connor , Pratul P. Srinivasan , Katherine L. Bouman

Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to…

Optics · Physics 2022-04-20 Herve Hugonnet , Mahn Jae Lee , Yongkeun Park

Distinguishing signal of young gas rich circumstellar disks from stellar signal in near infrared light is a difficult task. Current techniques such as Angular Differential Imaging (ADI) and Polarimetric Differential Imaging (PDI) cope with…

Instrumentation and Methods for Astrophysics · Physics 2022-12-07 L. M. Stapper , C. Ginski

Imaging through diffusers presents a challenging problem with various digital image reconstruction solutions demonstrated to date using computers. We present a computer-free, all-optical image reconstruction method to see through random…

A new numerically and experimentally tested measurement method for the local electrical light field including its phase is presented. The method is based on Self Referencing Interferograms (SRI) such as shearing interferograms. The complex…

Instrumentation and Detectors · Physics 2016-10-27 Martin Berz , Cordelia Berz

White light spectral interferometry is applied to measure the refractive index in absorbing liquids in the spectral range from 400 to 1000 nm. We analyze the influence of absorption on the visibility of interferometric fringes and,…

Fraunhofer diffraction is a well-known phenomenon achieved with most wavelength even without lens. A single-shot intensity measurement of diffraction is generally considered inadequate to reconstruct the original light field, because the…

Image and Video Processing · Electrical Eng. & Systems 2019-12-04 An-Dong Xiong , Xiao-Peng Jin , Wen-Kai Yu , Qing Zhao

Phase contrast imaging seeks to reconstruct the complex refractive index of an unknown sample from scattering intensities, measured for example under illumination with coherent X-rays. By incorporating refraction, this method yields…

Numerical Analysis · Mathematics 2018-11-06 Simon Maretzke

Quantitative phase imaging has become a topic of considerable interest in the microscopy community. We have recently described one such technique based on the use of a partitioned detection aperture, which can be operated in a single shot…

Optics · Physics 2015-10-20 Roman Barankov , Jean-Charles Baritaux , Jerome Mertz

We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with…

Phase retrieval, or the process of recovering phase information in reciprocal space to reconstruct images from measured intensity alone, is the underlying basis to a variety of imaging applications including coherent diffraction imaging…

Computer Vision and Pattern Recognition · Computer Science 2018-11-15 Mathew J. Cherukara , Youssef S. G. Nashed , Ross J. Harder

Optical Diffraction Tomography (ODT) is a powerful non-invasive imaging technique widely used in biological and medical applications. While significant progress has been made in transmission configuration, reflection ODT remains challenging…

Optics · Physics 2026-05-14 Thomas Wasik , Victor Barolle , Alexandre Aubry , Josselin Garnier

Phase-sensitive coherent imaging exploits changes in the phases of backscattered light to observe tiny alterations of scattering structures or variations of the refractive index. But moving scatterers or a fluctuating refractive index…

Reflective ptychography is a promising lensless imaging technique with a wide field of view, offering significant potential for applications in semiconductor manufacturing and detection. However, many semiconductor materials are coated with…

Optics · Physics 2025-01-03 Yun Gao , Qijun You , Peixiang Lu , Wei Cao

Quantitative phase imaging (QPI) quantifies the sample-specific optical-phase-delay enabling objective studies of optically-transparent specimens such as biological samples, but lacks chemical sensitivity limiting its application to…

Optics · Physics 2019-09-04 Miu Tamamitsu , Keiichiro Toda , Ryoichi Horisaki , Takuro Ideguchi

When used with coherent light, optical imaging systems, even diffraction-limited, are inherently unable to reproduce both the amplitude and the phase of a two-dimensional field distribution because their impulse response function varies…

Optics · Physics 2009-11-13 E. Brainis , C. Muldoon , L. Brandt , A. Kuhn

Quantitative phase imaging (QPI) enables label-free, high-contrast visualization of transparent specimens, but its common implementation in off-axis digital holographic microscopy (DHM) requires a separate reference beam, which increases…