Related papers: Fluholoscopy. Compact and Simple Platform Combinin…
The trade-off between throughput and image quality is an inherent challenge in microscopy. To improve throughput, compressive imaging under-samples image signals; the images are then computationally reconstructed by solving a regularized…
High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…
We report a compact, cost-effective and field-portable lensless imaging platform for quantitative microscopy. In this platform, the object is placed on top of an image sensor chip without using any lens. We use a low-cost galvo scanner to…
Medical image fusion is the process of registering and combining multiple images from single or multiple imaging modalities to improve the imaging quality and reduce randomness and redundancy in order to increase the clinical applicability…
Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…
By integrating a phase-only Spatial Light Modulator (SLM) into the illumination arm of a cylindrical-lens-based Selective Plane Illumination Microscope (SPIM), we have created a versatile system able to deliver high quality images by…
We describe the apparatus of a fluorescence optical microscope with both single-photon and two-photon non-diffracting light sheets excitation for large volume imaging. With special design to accommodate two different wavelength ranges…
We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of…
We report on amplitude and phase imaging of out-of-plane sinusoidal surface vibration at nanometer scales with a heterodyne holographic interferometer. The originality of the proposed method is to make use of a multiplexed local oscillator…
Conventional histopathology has long been essential for disease diagnosis, relying on visual inspection of tissue sections. Immunohistochemistry aids in detecting specific biomarkers but is limited by its single-marker approach, restricting…
Condensed phase systems often exhibit a mixture of deterministic and stochastic dynamics at the nanoscale which are essential to understanding their function, but can be challenging to study directly using conventional imaging methods.…
In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…
Time-resolved fluorescence imaging is a key tool in biomedical applications, as it allows to non-invasively obtain functional and structural information. However, the big amount of collected data introduces challenges in both acquisition…
We describe a method of low-coherence interferometry based optical profilometry using standard light-emitting diode (LED) illumination and complementary metal-oxide-semiconductor (CMOS) image sensors. A line-field illumination strategy…
In recent years nanoscale coherent imaging has emerged as an indispensable imaging modality allowing to surpass the resolution limit given by classical imaging optics. At the same time, attosecond science has experienced enormous progress…
We present a physically intuitive matrix approach for wave imaging and characterization in scattering media. The experimental proof-of-concept is performed with ultrasonic waves, but this approach can be applied to any field of wave physics…
Several matrix approaches were developed to control light propagation through multiple scattering media under illumination of ultrashort pulses of light. These matrices can be recorded either with spectral or temporal resolution. Thanks to…
We introduce a phase imaging mechanism for scanning transmission electron microscopy that exploits the complementary intensity changes of transmitted disks at different scattering angles. For scanning transmission electron microscopy, this…
Second harmonic generation (SHG) microscopy is a valuable tool for optical microscopy. SHG microscopy is normally performed as a point scanning imaging method, which lacks phase information and is limited in spatial resolution by the…
Analyzing microscopy images to extract biological object properties (e.g., their morphological organization, temporal dynamics, and population density) is fundamental to various biomedical research. Yet conducting this manually is costly…