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Tracking single fluorescent molecules has offered resolution into dynamic molecular processes at the single-molecule level. This perspective traces the evolution of single-molecule tracking, highlighting key developments across various…

Biological Physics · Physics 2026-02-24 Lance W. Q. Xu , Steve Pressé

Confocal and multi-photon microscopy are widely used for in-vivo fluorescence imaging of biological tissues such as the brain, offering non-invasive access up to ~1 mm depth without major loss in performance. A recently-developed…

Since the invention of digital cameras there has been a concerted drive towards detector arrays with higher spatial resolution. Microscanning is a technique that provides a final higher resolution image by combining multiple images of a…

Achieving high spatial resolution is the goal of many imaging systems. Designing a high-resolution lens with diffraction-limited performance over a large field of view remains a difficult task in imaging system design. On the other hand,…

A new algorithmic framework is presented for holographic phase retrieval via maximum likelihood optimization, which allows for practical and robust image reconstruction. This framework is especially well-suited for holographic coherent…

Image and Video Processing · Electrical Eng. & Systems 2022-02-18 David A. Barmherzig , Ju Sun

Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…

Optics · Physics 2016-03-25 Jaebum Chung , Hangwen Lu , Xiaoze Ou , Haojiang Zhou , Changhuei Yang

With the rapid development of microwave photonics, which has expanded to numerous applications of commercial importance, eliminating the emerging bottlenecks becomes of vital importance. For example, as the main branch of microwave…

Quantum Physics · Physics 2022-01-31 Yaqing Jin , Ye Yang , Huibo Hong , Xiao Xiang , Runai Quan , Tao Liu , Shougang Zhang , Ninghua Zhu , Ming Li , Ruifang Dong

Advanced microscopy techniques are essential in biomedical research for visualising and tracking biomolecules within living cells and their compartments. Conventional fluorescence microscopy methods, however, often struggle with accurately…

Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…

Raman microscopy is a valuable tool for detecting physical and chemical properties of a sample material. When probing nanomaterials or nanocomposites the spatial resolution of Raman microscopy is not always adequate as it is limited by the…

Visual inspection of solar modules is an important monitoring facility in photovoltaic power plants. Since a single measurement of fast CMOS sensors is limited in spatial resolution and often not sufficient to reliably detect small defects,…

Computer Vision and Pattern Recognition · Computer Science 2021-05-06 Mathis Hoffmann , Thomas Köhler , Bernd Doll , Frank Schebesch , Florian Talkenberg , Ian Marius Peters , Christoph J. Brabec , Andreas Maier , Vincent Christlein

Multiphoton microscopes employ femtosecond lasers as light sources because the high peak power of the ultrashort pulse allows for multiphoton excitation of fluorescence in the examined sample. However, such short pulses are susceptible to…

Optics · Physics 2024-08-13 Katarzyna Kunio , Jakub Bogusławski , Grzegorz Soboń

Large-area microscopy with submicron resolution is limited by tradeoffs between field of view (FOV), resolution, and imaging speed. Samples are rarely flat across centimeter-scale FOV, which often requires existing solutions to use…

Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution…

Advances in silicon photonics have resulted in rapidly increasing complexity of integrated circuits. New methods are desirable that allow direct characterization of individual optical components in-situ, without the need for additional…

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

In recent years several methods to overcome diffraction limit in the far field microscopy have been demonstrated. Still the problem of superresolution is reliably solved only for fluorescent microscopy, giving a resolution of up to 20-30nm.…

Optics · Physics 2013-09-03 Yu. V. Miklyaev , S. A. Asselborn , K. A. Zaytsev , M. Ya. Darscht

Neuromorphic imaging is an emerging technique that imitates the human retina to sense variations in dynamic scenes. It responds to pixel-level brightness changes by asynchronous streaming events and boasts microsecond temporal precision…

Image and Video Processing · Electrical Eng. & Systems 2024-10-23 Pei Zhang , Shuo Zhu , Chutian Wang , Yaping Zhao , Edmund Y. Lam

We present an advanced quantum super-resolution imaging technique based on photon statistics measurement and its accurate modeling. Our reconstruction algorithm adapts to any kind of non-Poissonian emitters, outperforming the corresponding…

We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…

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