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Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

We present the first three-dimensional theoretical model of microparticle-assisted super-resolution imaging, enabling accurate simulation of virtual image formation. The model reveals that accounting for partial spatial coherence of…

Second harmonic generation microscopy (SHG) is a powerful imaging modality which has found applications in investigating both biological and synthetic nanostructures. Like all optical microscopy techniques, the resolution of SHG is limited…

Mid-infrared photo-induced force microscopy (IR-PiFM/PiF-IR) enables high-resolution chemical imaging of surfaces with lateral resolution less than 5 nm. Here are some answers to questions about the physical background, practical handling…

Materials Science · Physics 2026-05-05 Daniela Täuber

Despite advances in low-light level detection, single-photon methods such as photon correlation have rarely been used in the context of imaging. The few demonstrations, for example of sub-diffraction limited imaging utilizing quantum…

Optics · Physics 2017-03-14 Yonatan Israel , Ron Tenne , Dan Oron , Yaron Silberberg

From biology and astronomy to quantum optics, there is a critical need for high frame rate, high quantum efficiency imaging. In practice, most cameras only satisfy one of these requirements. Here we introduce interlaced fast kinetics…

Atomic Physics · Physics 2025-05-21 Bowen Li , Lukas Palm , Marius Jürgensen , Yiming Cady Feng , Markus Greiner , Jon Simon

Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

Optics · Physics 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

We present a minimally-invasive endoscope based on a multimode fiber that combines photoacoustic and fluorescence sensing. From the measurement of a transmission matrix during a prior calibration step, a focused spot is produced and…

Imaging dynamical processes at interfaces and on the nanoscale is of great importance throughout science and technology. While light-optical imaging techniques often cannot provide the necessary spatial resolution, electron-optical…

Fourier ptychographic microscopy enables gigapixel-scale imaging, with both large field-of-view and high resolution. Using a set of low-resolution images that are recorded under varying illumination angles, the goal is to computationally…

Signal Processing · Electrical Eng. & Systems 2018-03-13 Emrah Bostan , Mahdi Soltanolkotabi , David Ren , Laura Waller

First discovered by Ernest Abbe in 1873, the resolution limit of a far-field microscope is considered determined by the numerical aperture and wavelength of light, approximately $\lambda$/2NA. With the advent of modern fluorescence…

Optics · Physics 2023-08-02 Yilun Li , Fang Huang

Multifocal microscopy (MFM) offers high-speed three-dimensional imaging through the simultaneous image capture from multiple focal planes. Conventional MFM systems use a fabricated grating in the emission path for a single emission…

Biological Physics · Physics 2019-07-31 M. Junaid Amin , Sabine Petry , Haw Yang , Joshua W. Shaevitz

The size- and fluorescence-based sorting of micro- and nano-scale particles suspended in fluid presents a significant and important challenge for both sample analysis and for manufacturing of nanoparticle-based products. Here we demonstrate…

Soft Condensed Matter · Physics 2015-02-16 Sukumar Rajauria , Christopher Axline , Claudia Gottstein , Andrew N. Cleland

We present an analytical description and an experimental realization of interscale mixing microscopy, a diffraction-based imaging technique that is capable of detecting wavelength/10 objects in far-field measurements with both coherent and…

A new far-field optical microscopy technique capable of reaching nanometer-scale resolution has been developed recently using the in-plane image magnification by surface plasmon polaritons. This microscopy is based on the optical properties…

Strongly Correlated Electrons · Physics 2009-11-10 Igor I. Smolyaninov

Spectroscopic photoemission microscopy is a well-established method to investigate the electronic structure of surfaces. In modern photoemission microscopes the electron optics allows imaging of the image plane, momentum plane, or…

The emergence of far-field super-resolution microscopy has rejuvenated the possibility for nanoscale imaging. Approaches to far-field super-resolution that utilize point scanning often depends on spatially reducing the size of the focused…

Optics · Physics 2022-06-17 Ning Xu , Guoxuan Liu , Qiaofeng Tan

The recent work by Chmyrov et al. (Nature Methods 2013) presents a capstone for the current major super-resolution microscopy techniques. In optical super-resolution microscopy, two pathways are commonly taken: targeted illumination…

Optics · Physics 2015-01-07 Xuanze Chen , Peng Xi
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