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Related papers: Confocal Raman Microscopy with Adaptive Optics

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Reflectance confocal microscopy (RCM) is an effective, non-invasive pre-screening tool for skin cancer diagnosis, but it requires extensive training and experience to assess accurately. There are few quantitative tools available to…

Computer Vision and Pattern Recognition · Computer Science 2017-12-04 Alican Bozkurt , Kivanc Kose , Jaume Coll-Font , Christi Alessi-Fox , Dana H. Brooks , Jennifer G. Dy , Milind Rajadhyaksha

Coherent anti-Stokes Raman scattering (CARS) microscopy offers label-free chemical imaging capabilities, but its performance is constrained by small Raman scattering cross-section, strong non-resonant background (NRB), and limited…

Time-resolved spectral techniques play an important analysis tool in many contexts, from physical chemistry to biomedicine. Customarily, the label-free detection of analytes is manually performed by experts through the aid of classic…

Machine Learning · Computer Science 2020-11-17 Stefano Rini , Hirotsugu Hiramatsu

Future large space telescopes will be equipped with adaptive optics (AO) to overcome wavefront aberrations and achieve high contrast for imaging faint astronomical objects, such as earth-like exoplanets and debris disks. In contrast to AO…

Instrumentation and Methods for Astrophysics · Physics 2020-01-07 He Sun , N. Jeremy Kasdin , Robert Vanderbei

Confocal and multi-photon microscopy are widely used for in-vivo fluorescence imaging of biological tissues such as the brain, offering non-invasive access up to ~1 mm depth without major loss in performance. A recently-developed…

Non-invasive morphological assessment is the cornerstone of oocyte and embryo selection in assisted reproductive technology, yet clinical practice remains limited by two-dimensional, qualitative microscopy. While three-dimensional (3D)…

In this paper, we present a new robotic system to perform defect inspection tasks over free-form specular surfaces. The autonomous procedure is achieved by a six-DOF manipulator, equipped with a line scan camera and a high-intensity…

Robotics · Computer Science 2020-08-26 Shengzeng Huo , David Navarro-Alarcon , David Chik

We report the modification of a label-free image scanning microscope (ISM) to perform asynchronous 2D imaging at 24kHz while keeping the lateral resolution gain and background rejection of a regular label-free ISM setup. Our method uses a…

Optics · Physics 2023-08-31 Duc-Minh Ta , Alberto Aguilar , Pierre Bon

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

In the realm of healthcare, label-free sensing is a vital component for various applications. Micro-photonic technology presents a promising avenue to pursue pivotal goals. With the use of this technology, healthcare professionals and…

Optics · Physics 2024-04-24 Giuseppina Simone

Aberrations limit optical systems in many situations, for example when imaging in biological tissue. Machine learning offers novel ways to improve imaging under such conditions by learning inverse models of aberrations. Learning requires…

Optics · Physics 2021-04-30 Ivan Vishniakou , Johannes D. Seelig

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive…

Biological Physics · Physics 2014-03-13 Charles-Edouard Leroux , Alexei Grichine , Irène Wang , Antoine Delon

Large-area microscopy with submicron resolution is limited by tradeoffs between field of view (FOV), resolution, and imaging speed. Samples are rarely flat across centimeter-scale FOV, which often requires existing solutions to use…

Raman spectroscopy's capability to provide meaningful composition predictions is heavily reliant on a pre-processing step to remove insignificant spectral variation. This is crucial in biofluid analysis. Widespread adoption of diagnostics…

Signal Processing · Electrical Eng. & Systems 2019-04-05 Emily E Storey , Amr S. Helmy

Coherent anti-Stokes Raman scattering is a widely used imaging technique that provides chemical contrast without the need for labels, making it an extremely valuable tool in physics, chemistry, and biology. In this work, we explore its…

Quantum Physics · Physics 2025-08-05 Giacomo Sorelli , Manuel Gessner , Frank Schlawin

Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…

High-resolution optical imaging methods, such as confocal microscopy and full-field optical coherence tomography, capture flat optical sections of the sample. If the sample is curved, the optical field sections through several sample layers…

Medical Physics · Physics 2020-07-31 Viacheslav Mazlin , Kristina Irsch , Michel Paques , Mathias Fink , Albert Claude Boccara

High-resolution wide field-of-view (FOV) microscopic imaging plays an essential role in various fields of biomedicine, engineering, and physical sciences. As an alternative to conventional lens-based scanning techniques, lensfree holography…

Optics · Physics 2017-06-16 Jialin Zhang , Jiasong Sun , Qian Chen , Jiaji Li , Chao Zuo

Widefield microscopy is widely used for non-invasive imaging of biological structures at subcellular resolution. When applied to complex specimen, its image quality is degraded by sample-induced optical aberration. Adaptive optics can…

Image and Video Processing · Electrical Eng. & Systems 2025-03-11 Iksung Kang , Qinrong Zhang , Stella X. Yu , Na Ji

Open-set image recognition is a challenging topic in computer vision. Most of the existing works in literature focus on learning more discriminative features from the input images, however, they are usually insensitive to the high- or…

Computer Vision and Pattern Recognition · Computer Science 2025-05-20 Qiulei Dong , Jiayin Sun , Mengyu Gao