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Structured illumination microscopy (SIM) is one of the most versatile super-resolution techniques. Yet, its application to live imaging has been so far mainly limited to fluorescent and stationary specimens. Here, we present advancements in…

We present a novel sparse signal reconstruction method "ISD", aiming to achieve fast reconstruction and a reduced requirement on the number of measurements compared to the classical l_1 minimization approach. ISD addresses failed…

Information Theory · Computer Science 2015-11-23 Yilun Wang , Wotao Yin

We present super-resolved coherent anti-Stokes Raman scattering (CARS) microscopy by implementing phase-resolved image scanning microscopy (ISM), achieving up to two-fold resolution increase as compared with a conventional CARS microscope.…

Optics · Physics 2024-11-22 Anna Zhitnitsky , Elad Benjamin , Ora Bitton , Dan Oron

In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint. This new algorithm is by far simpler and faster than…

Data Analysis, Statistics and Probability · Physics 2016-11-18 S. Labouesse , M. Allain , J. Idier , S. Bourguignon , A. Negash , P. Liu , A. Sentenac

In this paper, we present a new image segmentation method based on the concept of sparse subset selection. Starting with an over-segmentation, we adopt local spectral histogram features to encode the visual information of the small segments…

Computer Vision and Pattern Recognition · Computer Science 2018-04-10 Fariba Zohrizadeh , Mohsen Kheirandishfard , Farhad Kamangar

Fluorescence microscopy and derived techniques are continuously looking for photodetectors able to guarantee increased sensitivity, high spatial and temporal resolution and ease of integration into modern microscopy architectures. Recent…

In Super-resolution, a varying-illumination image stack is required. This enriched the dataset typically necessitates precise mechanical control and micron scale optical alignment and repeatability. Here, we introduce a novel methodology…

Conventional imaging only records photons directly sent from the object to the detector, while non-line-of-sight (NLOS) imaging takes the indirect light into account. Most NLOS solutions employ a transient scanning process, followed by a…

Computer Vision and Pattern Recognition · Computer Science 2021-03-29 Dayu Zhu , Wenshan Cai

Compressed sensing (CS) is a valuable technique for reconstructing measurements in numerous domains. CS has not yet gained widespread adoption in scanning tunneling microscopy (STM), despite potentially offering the advantages of lower…

Mesoscale and Nanoscale Physics · Physics 2022-02-09 Brian E. Lerner , Anayeli Flores-Garibay , Benjamin J. Lawrie , Petro Maksymovych

Structured illumination microscopy (SIM) has become an important technique for optical super-resolution imaging because it allows a doubling of image resolution at speeds compatible for live-cell imaging. However, the reconstruction of SIM…

Image and Video Processing · Electrical Eng. & Systems 2020-03-26 Charles N. Christensen , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Sparse sampling schemes have the potential to dramatically reduce image acquisition time while simultaneously reducing radiation damage to samples. However, for a sparse sampling scheme to be useful it is important that we are able to…

Computer Vision and Pattern Recognition · Computer Science 2017-03-16 G. M. Dilshan P. Godaliyadda , Dong Hye Ye , Michael D. Uchic , Michael A. Groeber , Gregery T. Buzzard , Charles A. Bouman

In traditional optical imaging systems, the spatial resolution is limited by the physics of diffraction, which acts as a low-pass filter. The information on sub-wavelength features is carried by evanescent waves, never reaching the camera,…

Optics · Physics 2018-12-13 Oren Solomon , Yonina C. Eldar , Maor Mutzafi , Mordechai Segev

High-content biological microscopy targets high-resolution imaging across large fields-of-view (FOVs). Recent works have demonstrated that computational imaging can provide efficient solutions for high-content microscopy. Here, we use…

Image and Video Processing · Electrical Eng. & Systems 2019-03-28 Li-Hao Yeh , Shwetadwip Chowdhury , Laura Waller

The objective of image super-resolution is to reconstruct a high-resolution (HR) image with the prior knowledge from one or several low-resolution (LR) images. However, in the real world, due to the limited complementary information, the…

Image and Video Processing · Electrical Eng. & Systems 2024-12-16 Jing Sun , Qiangqiang Yuan , Huanfeng Shen , Jie Li , Liangpei Zhang

This study proposes an algorithm based on a notch filter camera array system for simultaneous super-resolution imaging and spectral reconstruction, enhancing the spatial resolution and multispectral imaging capabilities of targets. In this…

Image and Video Processing · Electrical Eng. & Systems 2025-07-01 Peng Lin , Xuesong Wang , Yating Chen , Xianyu Wu , Feng Huang , Shouqian Chen

Structured illumination microscopy (SIM) improves resolution by down-modulating high-frequency information of an object to fit within the passband of the optical system. Generally, the reconstruction process requires prior knowledge of the…

Computer Vision and Pattern Recognition · Computer Science 2017-01-13 Li-Hao Yeh , Lei Tian , Laura Waller

We demonstrate a multi-beam scanning transmission electron microscopy (STEM) imaging that integrates down-sampling with super-resolution image reconstruction via a compressive sensing framework. A custom condenser aperture with six randomly…

Instrumentation and Detectors · Physics 2026-03-19 Akira Yasuhara , Takumi Sannomiya , Ryoichi Horisaki

Quantifying the number of molecules from fluorescence microscopy measurements is an important topic in cell biology and medical research. In this work, we present a consecutive algorithm for super-resolution (STED) scanning microscopy that…

Applications · Statistics 2023-10-03 Katharina Proksch , Frank Werner , Jan Keller-Findeisen , Haisen Ta , Axel Munk

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang