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Although several optical techniques have been recently developed in order to overcome the resolution limit in microscopy, the imaging of sub-wavelength features is still a real challenge. In practise, super-resolution techniques remain…

Physics Education · Physics 2019-07-24 Stephane Perrin , Keshia Badu , Paul Montgomery , Sylvain Lecler

A new method for improving the resolution of astronomical images is presented. It is based on the principle that sampled data cannot be fully deconvolved without violating the sampling theorem. Thus, the sampled image should not be…

Astrophysics · Physics 2009-10-30 P. Magain , F. Courbin , S. Sohy

Image Phase Alignment Super-sampling (ImPASS) is a computational method for combining displaced low-resolution images into a single high-resolution image. The general steps include measuring the relative displacements, up-sampling, aligning…

Optics · Physics 2026-01-09 James N. Caron

The principles of quantum optics have yielded a plethora of ideas to surpass the classical limitations of sensitivity and resolution in optical microscopy. While some ideas have been applied in proof-of-principle experiments, imaging a…

Optical microscopy has so far been restricted to superficial layers, leaving many important biological questions unanswered. Random scattering causes the ballistic focus, which is conventionally used for image formation, to decay…

Optics · Physics 2012-08-28 Ke Si , Reto Fiolka , Meng Cui

The resolution of optical imaging is classically limited by the width of the point-spread function, which in turn is determined by the Rayleigh length. Recently, spatial-mode demultiplexing (SPADE) has been proposed as a method to achieve…

Quantum Physics · Physics 2025-02-26 Giuseppe Buonaiuto , Cosmo Lupo

Conventional optical imaging is limited by diffraction, preventing discrimination of closely spaced incoherent sources. Inspired by quantum parameter estimation, this thesis explores spatial-mode demultiplexing (SPADE) as a method to…

Optics · Physics 2025-09-23 Nickolay Erin Titov

Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy,…

Optics · Physics 2024-10-11 Aymerick Bazin , Amaury Badon

Structured Illumination Microscopy is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep…

Image and Video Processing · Electrical Eng. & Systems 2021-02-19 Miguel Boland , Edward A. K. Cohen , Seth Flaxman , Mark A. A. Neil

We introduce a superresolution technique that combines spatial mode demultiplexing (SPADE) with emitter blinking. We show that temporal fluctuations not only enhance the precision of SPADE imaging, but also drastically simplify the…

Optics · Physics 2025-11-27 Stanislaw Kurdzialek

This paper presents a comprehensive numerical analysis of super-resolution imaging using dielectric microparticles, employing the Finite-Difference Time-Domain (FDTD) method to elucidate the mechanisms that enable resolution enhancements…

Optics · Physics 2024-09-18 Arlen Bekirov , Zengbo Wang , Boris Lukyanchuk

I propose a spatial-mode demultiplexing (SPADE) measurement scheme for the far-field imaging of spatially incoherent optical sources. For any object too small to be resolved by direct imaging under the diffraction limit, I show that SPADE…

Optics · Physics 2017-03-01 Mankei Tsang

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

In the past years modern mathematical methods for image analysis have led to a revolution in many fields, from computer vision to scientific imaging. However, some recently developed image processing techniques successfully exploited by…

Instrumentation and Methods for Astrophysics · Physics 2015-01-19 Marco Castellano , Daniele Ottaviani , Adriano Fontana , Emiliano Merlin , Stefano Pilo , Maurizio Falcone

The main advantage of two-photon fluorescence confocal microscopy is the low absorption obtained with live tissues at the wavelengths of operation. However, the resolution of two-photon fluorescence confocal microscopes is lower than in the…

Biological Physics · Physics 2009-11-13 N. Sandeau , H. Giovannini

Improvement of label-free far-field resolution of optical imaging is possible with prior knowledge of the object such as its sparsity or accumulated by a posteriori examination of a similar class of object1-4. We show that the sole…

Optics · Physics 2026-02-03 Taeyong Chang , Giorgio Adamo , Nikolay I. Zheludev

Since the invention of digital cameras there has been a concerted drive towards detector arrays with higher spatial resolution. Microscanning is a technique that provides a final higher resolution image by combining multiple images of a…

Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

Biological Physics · Physics 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

The emerging optical multiplexing within nanoscale shows super-capacity in encoding information by using the time-domain fingerprints from uniform nanoparticles. However, the optical diffraction limit compromises the decoding throughput and…

Optics · Physics 2022-08-09 Baolei Liu , Jiayan Liao , Yiliao Song , Jie Lu , Jiajia Zhou , Fan Wang