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The most ubiquitous form of computational aberration correction for microscopy is deconvolution. However, deconvolution relies on the assumption that the point spread function is the same across the entire field-of-view. This assumption is…
We demonstrate a significant resolution enhancement beyond the conventional limit in multiphoton microscopy (MPM) using saturated excitation of fluorescence. Our technique achieves super-resolved imaging by temporally modulating the…
Many lighting methods used in computer graphics such as indirect illumination can have very high computational costs and need to be approximated for real-time applications. These costs can be reduced by means of upsampling techniques which…
Most satellites decouple the acquisition of a panchromatic image at high spatial resolution from the acquisition of a multispectral image at lower spatial resolution. Pansharpening is a fusion technique used to increase the spatial…
Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…
Confocal microscopy has long been a cornerstone technique for visualizing complex interactions and processes within cellular structures. However, achieving super-resolution imaging of multiple organelles and their interactions…
It was proposed that a flat silver layer could be used to form a sub-diffraction limited image when illuminated near its surface plasmon resonance frequency [J. B. Pendry, Phys. Rev. Lett. 86, 3966 (2000)]. In this paper, we study the…
Recent developments in image acquisition literature have miniaturized the confocal laser endomicroscopes to improve usability and flexibility of the apparatus in actual clinical settings. However, miniaturized devices collect less light and…
Recently, imaging by microspheres and dielectric particle-lenses emerged as a simple solution to obtaining super-resolution images of nanoscale devices and structures. Calibrated resolution of ~{\lambda}/6 - {\lambda}/8 has been…
High-throughput 2D and 3D scanning electron microscopy, which relies on automation and dependable control algorithms, requires high image quality with minimal human intervention. Classical focus and astigmatism correction algorithms attempt…
Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…
Imaging in thick biological tissues is often degraded by sample-induced aberrations, which reduce image quality and resolution, particularly in super-resolution techniques. While hardware-based adaptive optics, which correct aberrations…
A resolution-enhanced dark-field microscope by structured light illumination is proposed to improve resolution and contrast. A set of phase-shifted fringes are projected to the sample plane at large angle to capture modulated dark-field…
Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…
Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…
We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…
I explain what is, what is not, and what is only sort of superresolution microscopy. I discuss optical resolution, first in terms of diffraction theory, then in terms of linear systems theory, and finally in terms of techniques that use…
This article presents a method to perform diffraction tomography in a standard microscope that includes an LED array for illumination. After acquiring a sequence of intensity-only images of a thick sample, a ptychography-based…
In 2011, super-resolution imaging by microsphere superlens was emerged as a simple yet effective method to overcome the diffraction limit that limits the resolution of conventional lenses. Significant progress has since been made. Key…
Superresolution is one of the key issues at the crossroads of contemporary quantum optics and metrology. Recently, it was shown that for an idealized case of two balanced sources, spatial mode demultiplexing (SPADE) achieves resolution…